Objective： To explore the brain function mechanism of ＂liver-soothing and mind-regulating＂ acupuncture manipulation in intervening post-traumatic stress disorder（PTSD）.Methods： According to random number table m...Objective： To explore the brain function mechanism of ＂liver-soothing and mind-regulating＂ acupuncture manipulation in intervening post-traumatic stress disorder（PTSD）.Methods： According to random number table method, 60 SD rats were randomly divided into blank group, model group, grabbing group, paroxetine group and acupuncture group. Except for the blank group, all the rats were established into PTSD models through electric shock and incarceration, with model replication for 7 days in total. The rats were given gavage, acupuncture intervention and grabbing fixation 1 h before modeling, with 6 days as 1 course of treatment. Grabbing treatment was performed for 2 courses, with 12 days in total. After modeling, functional near-infrared spectroscopy（fNIRS） system was adopted to collect and record the changes of concentration of Oxy-Hb, Deoxy-Hb and Total-Hb during 3 min in related brain regions of rats in each group after treatment for 5 consecutive days, and the brain function was evaluated.Results： According to the comparison of the time series of blood-oxygen concentration, there were significant differences between model group and blank group as well as grabbing group and blank group（P〈0.01, P〈0.01）, no significant difference was found between model group and grabbing group（P〈0.05）; there were significant differences between paroxetine group and grabbing group as well as acupuncture group and grabbing group（P〈0.05, P〈0.05）, no significant difference was found between paroxetine group and acupuncture group（P〈0.05）.Conclusion： ＂Liver-soothing and mind-regulating＂ acupuncture manipulation has a benign regulatory effect on the blood-oxygen concentration in cerebral cortex of PTSD rats, which may be the important brain function mechanism of ＂liver-soothing and mind-regulating＂ acupuncture manipulation in intervening PTSD.展开更多
基金Supported by Regional fund project of 2014 National Natural Science Foundation of China:814607442013 Gansu Provincial Natural Science Foundation:1308RJZA150~~
文摘Objective： To explore the brain function mechanism of ＂liver-soothing and mind-regulating＂ acupuncture manipulation in intervening post-traumatic stress disorder（PTSD）.Methods： According to random number table method, 60 SD rats were randomly divided into blank group, model group, grabbing group, paroxetine group and acupuncture group. Except for the blank group, all the rats were established into PTSD models through electric shock and incarceration, with model replication for 7 days in total. The rats were given gavage, acupuncture intervention and grabbing fixation 1 h before modeling, with 6 days as 1 course of treatment. Grabbing treatment was performed for 2 courses, with 12 days in total. After modeling, functional near-infrared spectroscopy（fNIRS） system was adopted to collect and record the changes of concentration of Oxy-Hb, Deoxy-Hb and Total-Hb during 3 min in related brain regions of rats in each group after treatment for 5 consecutive days, and the brain function was evaluated.Results： According to the comparison of the time series of blood-oxygen concentration, there were significant differences between model group and blank group as well as grabbing group and blank group（P〈0.01, P〈0.01）, no significant difference was found between model group and grabbing group（P〈0.05）; there were significant differences between paroxetine group and grabbing group as well as acupuncture group and grabbing group（P〈0.05, P〈0.05）, no significant difference was found between paroxetine group and acupuncture group（P〈0.05）.Conclusion： ＂Liver-soothing and mind-regulating＂ acupuncture manipulation has a benign regulatory effect on the blood-oxygen concentration in cerebral cortex of PTSD rats, which may be the important brain function mechanism of ＂liver-soothing and mind-regulating＂ acupuncture manipulation in intervening PTSD.
