Upon recognition of dsRNA,toll-like receptor 3(TLR3)recruits the adaptor protein TRIF to activate IRF3 and NF-κB signaling,initiating innate immune responses.The ubiquitination of TLR3 downstream signaling molecules ...Upon recognition of dsRNA,toll-like receptor 3(TLR3)recruits the adaptor protein TRIF to activate IRF3 and NF-κB signaling,initiating innate immune responses.The ubiquitination of TLR3 downstream signaling molecules and their roles in the innate response have been discovered;however,whether TLR3 itself is ubiquitinated and then functionally involved remains to be elucidated.By immunoprecipitating TLR3-binding proteins in macrophages,we identified ring finger protein 170(RNF170)as a TLR3-binding E3 ligase.RNF170 mediated the K48-linked polyubiquitination of K766 in the TIR domain of TLR3 and promoted the degradation of TLR3 through the proteasome pathway.The genetic ablation of RNF170 selectively augmented TLR3-triggered innate immune responses both in vitro and in vivo.Our results reveal a novel role for RNF170 in selectively inhibiting TLR3-triggered innate immune responses by promoting TLR3 degradation.展开更多
Visible-light-driven photocatalysis as a green technology has attracted a lot of attention due to its potential applications in environmental remediation. Vesicle Cd Se nano-semiconductor photocatalyst are successfull...Visible-light-driven photocatalysis as a green technology has attracted a lot of attention due to its potential applications in environmental remediation. Vesicle Cd Se nano-semiconductor photocatalyst are successfully prepared by a gas template method and characterized by a variety of methods. The vesicle Cd Se nano-semiconductors display enhanced photocatalytic performance for the degradation of tetracycline hydrochloride, the photodegradation rate of78.824% was achieved by vesicle Cd Se, which exhibited an increase of 31.779% compared to granular Cd Se. Such an exceptional photocatalytic capability can be attributed to the unique structure of the vesicle Cd Se nano-semiconductor with enhanced light absorption ability and excellent carrier transport capability. Meanwhile, the large surface area of the vesicle Cd Se nano-semiconductor can increase the contact probability between catalyst and target and provide more surface-active centers. The photocatalytic mechanisms are analyzed by active species quenching. It indicates that h+and UO2^-are the main active species which play a major role in catalyzing environmental toxic pollutants. Simultaneously, the vesicle Cd Se nano-semiconductor had high efficiency and stability.展开更多
基金This work was supported by grants from the National Natural Science Foundation of China(grant 81788101 to X.C.grant 81871236 to M.J.)the National 135 Mega Program of China(grant 2017ZX10202203-002 to M.J.grant 2017ZX10203206-001 to X.C.)+1 种基金the CAMS Innovation Fund for Medical Sciences(grant 2016-12M-1-003 to X.C.)the Medical Epigenetics Research Center,Chinese Academy of Medical Sciences(2018PT31015).
文摘Upon recognition of dsRNA,toll-like receptor 3(TLR3)recruits the adaptor protein TRIF to activate IRF3 and NF-κB signaling,initiating innate immune responses.The ubiquitination of TLR3 downstream signaling molecules and their roles in the innate response have been discovered;however,whether TLR3 itself is ubiquitinated and then functionally involved remains to be elucidated.By immunoprecipitating TLR3-binding proteins in macrophages,we identified ring finger protein 170(RNF170)as a TLR3-binding E3 ligase.RNF170 mediated the K48-linked polyubiquitination of K766 in the TIR domain of TLR3 and promoted the degradation of TLR3 through the proteasome pathway.The genetic ablation of RNF170 selectively augmented TLR3-triggered innate immune responses both in vitro and in vivo.Our results reveal a novel role for RNF170 in selectively inhibiting TLR3-triggered innate immune responses by promoting TLR3 degradation.
基金supported by the National Natural Science Foundation of China (Nos. 21546013, U1510126, 21407064)the Natural Science Foundation of Jiangsu Province (No. BK20131259)+3 种基金the China Postdoctoral Science Foundation (No. 2015 M571684)the Jiangsu Postdoctoral Science Foundation (No. 1501102B)the Innovation Programs Foundation of Jiangsu Province (No. KYLX15_1089)the Open Research Fund of State Key Laboratory of Coal Resources and Safe Mining, China University of Mining and Technology (No. 12KF09)
文摘Visible-light-driven photocatalysis as a green technology has attracted a lot of attention due to its potential applications in environmental remediation. Vesicle Cd Se nano-semiconductor photocatalyst are successfully prepared by a gas template method and characterized by a variety of methods. The vesicle Cd Se nano-semiconductors display enhanced photocatalytic performance for the degradation of tetracycline hydrochloride, the photodegradation rate of78.824% was achieved by vesicle Cd Se, which exhibited an increase of 31.779% compared to granular Cd Se. Such an exceptional photocatalytic capability can be attributed to the unique structure of the vesicle Cd Se nano-semiconductor with enhanced light absorption ability and excellent carrier transport capability. Meanwhile, the large surface area of the vesicle Cd Se nano-semiconductor can increase the contact probability between catalyst and target and provide more surface-active centers. The photocatalytic mechanisms are analyzed by active species quenching. It indicates that h+and UO2^-are the main active species which play a major role in catalyzing environmental toxic pollutants. Simultaneously, the vesicle Cd Se nano-semiconductor had high efficiency and stability.