The economic value of a black walnut (Juglans nigra L.) tree is strongly determined by the quality and quantity of darkly colored heartwood in its stem. To understand the regulation of heartwood formation, we analyzed...The economic value of a black walnut (Juglans nigra L.) tree is strongly determined by the quality and quantity of darkly colored heartwood in its stem. To understand the regulation of heartwood formation, we analyzed the region of heartwood formation in walnut stems (i.e., the transition zone, TZ) for the expression of 80 ESTs. Semi-quantitative RT-PCR and real-time PCR was performed to detect expression changes of candidate genes in the TZ and sapwood of trees harvested in summer and fall. Results revealed that the transcript of a clone containing two presumed EF-hand motifs was expressed at higher levels in the TZ than in other xylem tissues. Analysis of the full-length coding sequence revealed that the black walnut gene JnCML-like is similar to grancalcin-like calcium-binding EF hand proteins in Arabidopsis thaliana (At3g10300) and Zea mays (NM 001153810). A model of the predicted structure of JnCML-like showed it is similar to grancalcin and m-calpain, penta-EF-hand family proteins associated with cell proliferation, differentiation and programmed cell death. JnCML-like transcript was detected in tissue from the region of the pith meristem, and in roots, embryogenic callus, vascular cambium, female flowers, male flowers, green leaves, and partially and fully senescent leaves of black walnut, although transcript abundance varied considerably among these tissues.展开更多
To the Editor:Neurovascular injury is an uncommon complication of clavicle fractures,and occurs in at most 1%of all clavicle fractures.Neurovascular injury is mostly caused by high-energy blunt injury to the clavicle....To the Editor:Neurovascular injury is an uncommon complication of clavicle fractures,and occurs in at most 1%of all clavicle fractures.Neurovascular injury is mostly caused by high-energy blunt injury to the clavicle.[1]Because its symptoms are less prominent,these vascular injuries are easily neglected and may result in severe consequences.However,few studies have reported a standardized management for this rare trauma.Here,we present a case of a 30-year-old man who suffered an open clavicle fracture with acute subclavian artery and brachial plexus injuries.After treating this case,we concluded that neurovascular examinations and multidisciplinary therapeutic strategies are urgently needed for clavicle fractures with associated neurovascular injury.The authors certify that they have obtained the patient’s consent to the publication of information about him in a journal or thesis.The information will be published without the patient’s name attached and every attempt will be made to ensure anonymity.展开更多
文摘The economic value of a black walnut (Juglans nigra L.) tree is strongly determined by the quality and quantity of darkly colored heartwood in its stem. To understand the regulation of heartwood formation, we analyzed the region of heartwood formation in walnut stems (i.e., the transition zone, TZ) for the expression of 80 ESTs. Semi-quantitative RT-PCR and real-time PCR was performed to detect expression changes of candidate genes in the TZ and sapwood of trees harvested in summer and fall. Results revealed that the transcript of a clone containing two presumed EF-hand motifs was expressed at higher levels in the TZ than in other xylem tissues. Analysis of the full-length coding sequence revealed that the black walnut gene JnCML-like is similar to grancalcin-like calcium-binding EF hand proteins in Arabidopsis thaliana (At3g10300) and Zea mays (NM 001153810). A model of the predicted structure of JnCML-like showed it is similar to grancalcin and m-calpain, penta-EF-hand family proteins associated with cell proliferation, differentiation and programmed cell death. JnCML-like transcript was detected in tissue from the region of the pith meristem, and in roots, embryogenic callus, vascular cambium, female flowers, male flowers, green leaves, and partially and fully senescent leaves of black walnut, although transcript abundance varied considerably among these tissues.
基金Guangdong Basic and Applied Basic Research Foundation(Nos.2021A1515011285,2019A1515010800)Major Project under the Science and Technology Development Scheme of Guangdong Province(Nos.210715106900918,[2020]53-129)Shantou Science and Technology Plan Medical and Health Category Project(No.211114216492935)
文摘To the Editor:Neurovascular injury is an uncommon complication of clavicle fractures,and occurs in at most 1%of all clavicle fractures.Neurovascular injury is mostly caused by high-energy blunt injury to the clavicle.[1]Because its symptoms are less prominent,these vascular injuries are easily neglected and may result in severe consequences.However,few studies have reported a standardized management for this rare trauma.Here,we present a case of a 30-year-old man who suffered an open clavicle fracture with acute subclavian artery and brachial plexus injuries.After treating this case,we concluded that neurovascular examinations and multidisciplinary therapeutic strategies are urgently needed for clavicle fractures with associated neurovascular injury.The authors certify that they have obtained the patient’s consent to the publication of information about him in a journal or thesis.The information will be published without the patient’s name attached and every attempt will be made to ensure anonymity.
