To analyse the genotypes of clinical isolates of Extended-Spectrum-β-Lactamase-Producing (ESBL-producing) Proteus mirabilis (P. mirabilis) and the mechanisms of antimicrobial resistance, to guide reasonable use of an...To analyse the genotypes of clinical isolates of Extended-Spectrum-β-Lactamase-Producing (ESBL-producing) Proteus mirabilis (P. mirabilis) and the mechanisms of antimicrobial resistance, to guide reasonable use of antibiotics and to avoid nosocomial outbreak infections by ESBL-producing P. mirabilis. 125 clinical isolates of P. mirabilis were collected from the Drug-Resistant Bacteria Surveillance Center of Anhui Province (from Jan 2009 to May 2010). Searching for the genotypes of ESBLs was perfomed by PCR amplification and DNA sequencing, and performed conjugation test simultaneously. Among ESBL-producing strains, CTX-M was the major genotype (3 CTX-M-13 and 1 CTX-M-3). TEM-1b spectrum β-lactamase was also prevalence in P. mirabilis. The diversity of β-lactamases in P. mirabilis and the emergency of multi-drug-resistance clinical strains will present serious threat to clinical therapy and even will lead to outbreak of nosocomial infections. Our study emphasizes the need for enhanced supervision of ESBL-producing P. mirabilis. Timely and reasonable drug-resistance data are indispensable to clinical therapy.展开更多
The study of the history of human activities in ancient cities has provided valuable evidences for under- standing the evolution of human-land relations during the late Holocene. Numerous ancient cities were discovere...The study of the history of human activities in ancient cities has provided valuable evidences for under- standing the evolution of human-land relations during the late Holocene. Numerous ancient cities were discovered through archaeological surveys of the east Qinghai Province, located on the northeastern border of the Tibetan Plateau, China; however, the mystery of when or why these cities were built remains unsolved. As recorded in this paper, we sampled reliable dating materials from 47 ancient cities in the area, determined their ages by radiocarbon dating, and compared the dating results with historical documents and high resolution paleoclimate records to explore the influencing factors for the develop- ment of these ancient cities. The 54 radiocarbon dates indicated that most of these cities were built or repaired during the Han Dynasty （202 BC-AD 220）, Tang Dynasty （AD 618-AD 907）, the Five Dynasties and Ten Kingdoms period （AD 907-AD 960）, the Song dynasty （AD 960-AD 1279）, and the Ming Dynasty （AD 1368-AD 1644）. The radiocarbon dates correspond well with historical records of the area. Our work suggests the ancient cities in east Qinghai Province were likely built primarily for military defense, and may have also have been affected by climate change.展开更多
文摘To analyse the genotypes of clinical isolates of Extended-Spectrum-β-Lactamase-Producing (ESBL-producing) Proteus mirabilis (P. mirabilis) and the mechanisms of antimicrobial resistance, to guide reasonable use of antibiotics and to avoid nosocomial outbreak infections by ESBL-producing P. mirabilis. 125 clinical isolates of P. mirabilis were collected from the Drug-Resistant Bacteria Surveillance Center of Anhui Province (from Jan 2009 to May 2010). Searching for the genotypes of ESBLs was perfomed by PCR amplification and DNA sequencing, and performed conjugation test simultaneously. Among ESBL-producing strains, CTX-M was the major genotype (3 CTX-M-13 and 1 CTX-M-3). TEM-1b spectrum β-lactamase was also prevalence in P. mirabilis. The diversity of β-lactamases in P. mirabilis and the emergency of multi-drug-resistance clinical strains will present serious threat to clinical therapy and even will lead to outbreak of nosocomial infections. Our study emphasizes the need for enhanced supervision of ESBL-producing P. mirabilis. Timely and reasonable drug-resistance data are indispensable to clinical therapy.
基金Acknowledgements This research was funded by the National Social Science Foundation of China （Grant Nos. 12＆ZD 151 and 12XKG006）, the National Natural Science Foundation of China （Grant Nos. 41271218）, Fundamental Research Funds for the Central Universities （lzujbky-2015-k09, lzujbky-2014-116）, as well as the 111 Program （#B06026） of Chinese State Administration of Foreign Experts Affairs. We would like to thank Dr. Emma LIGHFOOT for improving the English, and Mr. Zhilin Shi for providing historical documents.
文摘The study of the history of human activities in ancient cities has provided valuable evidences for under- standing the evolution of human-land relations during the late Holocene. Numerous ancient cities were discovered through archaeological surveys of the east Qinghai Province, located on the northeastern border of the Tibetan Plateau, China; however, the mystery of when or why these cities were built remains unsolved. As recorded in this paper, we sampled reliable dating materials from 47 ancient cities in the area, determined their ages by radiocarbon dating, and compared the dating results with historical documents and high resolution paleoclimate records to explore the influencing factors for the develop- ment of these ancient cities. The 54 radiocarbon dates indicated that most of these cities were built or repaired during the Han Dynasty （202 BC-AD 220）, Tang Dynasty （AD 618-AD 907）, the Five Dynasties and Ten Kingdoms period （AD 907-AD 960）, the Song dynasty （AD 960-AD 1279）, and the Ming Dynasty （AD 1368-AD 1644）. The radiocarbon dates correspond well with historical records of the area. Our work suggests the ancient cities in east Qinghai Province were likely built primarily for military defense, and may have also have been affected by climate change.
