在沙棘籽油提取过程中,经粉碎干燥后的籽料颗粒是一种开放体系,脂滴以游离形式存在,采用油红O(Oil Red O,ORO)对其进行染色时,常规方法一次性滴加染液的方式以及后续的洗脱步骤均会导致脂滴迁移、过染等问题出现。研究构建了逐级染色法...在沙棘籽油提取过程中,经粉碎干燥后的籽料颗粒是一种开放体系,脂滴以游离形式存在,采用油红O(Oil Red O,ORO)对其进行染色时,常规方法一次性滴加染液的方式以及后续的洗脱步骤均会导致脂滴迁移、过染等问题出现。研究构建了逐级染色法,采用少量多次滴加染液的方式,并优化了染色间隔时间为1.5 min,染色级数和染液浓度可根据样品的实际油含量进行调整,因而能够客观地显影脂滴在籽料颗粒内的分布状态。以此构建的面积占比法可用于精确测定籽料中的油脂含量,其准确度与萃取质量法相当,两者的相关系数为0.998,并具有较高重现性,相对标准偏差约为2.49%。展开更多
本文提出了一种基于㶲分析的层次分析-模糊评价法(analytic hierarchy process and fuzzy comprehensive evaluation,AHP-FCE),并利用该方法对村镇固体废弃物的固定床气化工艺进行评价、预测和优选.首先,利用㶲分析法计算出气化系统的㶲效...本文提出了一种基于㶲分析的层次分析-模糊评价法(analytic hierarchy process and fuzzy comprehensive evaluation,AHP-FCE),并利用该方法对村镇固体废弃物的固定床气化工艺进行评价、预测和优选.首先,利用㶲分析法计算出气化系统的㶲效率;其次,结合AHP法和FCE法从㶲效率、产气率、产气LHV、温室气体和废渣排放量5种评价指标对气化工艺进行综合性评价.结果表明:当T=1000℃、ER=0.35时,该气化系统的综合评分最高;氧气-水蒸气气化工艺优于空气气化和水蒸气气化;上吸式空气气化和下吸式氧气-水蒸气气化评分较高,即为最优的村镇固体废弃物固定床气化工艺.展开更多
In the mid-latitude regions of the Asian continent, Zhuye Lake is located in the northwest margin of the Asian monsoon, where the modem climate is affected by the Asian monsoon and Westerlies. In this study, we invest...In the mid-latitude regions of the Asian continent, Zhuye Lake is located in the northwest margin of the Asian monsoon, where the modem climate is affected by the Asian monsoon and Westerlies. In this study, we investi- gated the absolutely dated Holocene records in Zhuye Lake for detecting the Holocene climate cycles. Totally, 14 14C dates and 6 optically simulated luminescence （OSL） dates are obtained from the QTH01 and QTH02 sections. The proxies of grain-size, total organic carbon content （TOC）, C/N and 813C are used for wavelet analysis, and the results show obvious -256, -512 and -1024-year climate cycles, which are consistent with the Holocene millennial and cen- tennial scale climate cycles in the typical Asian summer monsoon domain. In different parts of the Zhuye Lake, the Holocene sediments show variable climate cycles that are affected by the lake basin topography. In the Zhuye Lake, the Holocene climate cycles are mainly correlated with the solar-related Asian summer monsoon variability and the North Atlantic ice-rafting events.展开更多
目的对2021年云南省文山州手足口病(hand,foot and mouth disease,HFMD)病例标本进行基因检测并对其中检测到的1株柯萨奇病毒A组5型(coxsackievirus A5,CVA5)的基因特征进行分析,为今后对该病毒的防控提供分子流行病学数据。方法采集202...目的对2021年云南省文山州手足口病(hand,foot and mouth disease,HFMD)病例标本进行基因检测并对其中检测到的1株柯萨奇病毒A组5型(coxsackievirus A5,CVA5)的基因特征进行分析,为今后对该病毒的防控提供分子流行病学数据。方法采集2021年文山州手足口病病例粪便和咽拭子标本,并对经反转录-聚合酶链式反应法鉴定的其他肠道病毒进行基因测序定型。直接从处理后的标本中提取病毒RNA,先用MD91/OL68-1引物扩增病毒VP4/VP2结合区基因并进行病毒鉴定,再用肠道病毒特异性引物扩增VP1区完整基因,用DNAStar软件中的Seqman程序对病毒基因进行编辑、整理,得到其完整VP1区序列。下载CVA5病毒VP1基因代表株参考序列,用MEGA 5.2软件进行基因特征分析。结果2021年文山州共报告手足口病病例6017例,发病率171.73/10万,其中,经实验室反转录-聚合酶链式反应法确诊的429例手足口病病例中,EV-A7157例,占13.29%(57/429),CVA16137例,占31.93%(137/429),其他肠道病毒235例,占54.78%(235/429)。用基因测序法从235例其他肠道病毒中检测到1株CVA5,检出率为0.43%(1/235)。此株CVA5为C基因型。结论2021年云南省文山州手足口病病原体以其他肠道病毒为主,检测到1株CVA5病毒,为C基因型。CVA5病毒可能是文山州手足口病的稀有病原,该病毒在文山州的发现值得注意。展开更多
文摘在沙棘籽油提取过程中,经粉碎干燥后的籽料颗粒是一种开放体系,脂滴以游离形式存在,采用油红O(Oil Red O,ORO)对其进行染色时,常规方法一次性滴加染液的方式以及后续的洗脱步骤均会导致脂滴迁移、过染等问题出现。研究构建了逐级染色法,采用少量多次滴加染液的方式,并优化了染色间隔时间为1.5 min,染色级数和染液浓度可根据样品的实际油含量进行调整,因而能够客观地显影脂滴在籽料颗粒内的分布状态。以此构建的面积占比法可用于精确测定籽料中的油脂含量,其准确度与萃取质量法相当,两者的相关系数为0.998,并具有较高重现性,相对标准偏差约为2.49%。
文摘本文提出了一种基于㶲分析的层次分析-模糊评价法(analytic hierarchy process and fuzzy comprehensive evaluation,AHP-FCE),并利用该方法对村镇固体废弃物的固定床气化工艺进行评价、预测和优选.首先,利用㶲分析法计算出气化系统的㶲效率;其次,结合AHP法和FCE法从㶲效率、产气率、产气LHV、温室气体和废渣排放量5种评价指标对气化工艺进行综合性评价.结果表明:当T=1000℃、ER=0.35时,该气化系统的综合评分最高;氧气-水蒸气气化工艺优于空气气化和水蒸气气化;上吸式空气气化和下吸式氧气-水蒸气气化评分较高,即为最优的村镇固体废弃物固定床气化工艺.
基金Under the auspices of National Natural Science Foundation of China (No. 41001116)Fundamental Research Funds for the Central Universities (No. LZUJBKY-2010-99)
文摘In the mid-latitude regions of the Asian continent, Zhuye Lake is located in the northwest margin of the Asian monsoon, where the modem climate is affected by the Asian monsoon and Westerlies. In this study, we investi- gated the absolutely dated Holocene records in Zhuye Lake for detecting the Holocene climate cycles. Totally, 14 14C dates and 6 optically simulated luminescence （OSL） dates are obtained from the QTH01 and QTH02 sections. The proxies of grain-size, total organic carbon content （TOC）, C/N and 813C are used for wavelet analysis, and the results show obvious -256, -512 and -1024-year climate cycles, which are consistent with the Holocene millennial and cen- tennial scale climate cycles in the typical Asian summer monsoon domain. In different parts of the Zhuye Lake, the Holocene sediments show variable climate cycles that are affected by the lake basin topography. In the Zhuye Lake, the Holocene climate cycles are mainly correlated with the solar-related Asian summer monsoon variability and the North Atlantic ice-rafting events.
