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Preparation and Application of Polymyxin B Affinity Membrane Cartridge 被引量:1
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作者 Guo, W Shang, ZH +2 位作者 Yu, YN zhou, lm zhou, K 《Chinese Chemical Letters》 SCIE CAS CSCD 1997年第8期745-746,共2页
Affinity membrane was used for the endotoxin adsorption. The matrix macropore membrane was prepared from filterpaper by chemically crosslinking, and polymyxin B was immobilized as affinity ligand. The prepared membran... Affinity membrane was used for the endotoxin adsorption. The matrix macropore membrane was prepared from filterpaper by chemically crosslinking, and polymyxin B was immobilized as affinity ligand. The prepared membrane cartridge can be used for the removal of endotoxin from aqueous solutions. With a cartridge of 40 sheets affinity membrane containing, the endotoxin level of the processed solution can be reduced to a minimum of 4 pg/mL. 展开更多
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ENDOTOXIN REMOVAL BY MEMBRANE AFFINITY CHROMATOGRAPHY
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作者 Guo, W Shang, ZH +4 位作者 Yu, YN zhou, lm Zun, ZY Ma, LR Chen, MY 《Chinese Chemical Letters》 SCIE CAS CSCD 1995年第10期919-922,共4页
Endotoxin was removed by membrane affinity chromatography. Macropore cellulose membrane was prepared from filter paper by alkaline treatment and chemical crosslinking, and was used as stationary phase for affinity chr... Endotoxin was removed by membrane affinity chromatography. Macropore cellulose membrane was prepared from filter paper by alkaline treatment and chemical crosslinking, and was used as stationary phase for affinity chromatography. The matrix membrane was derived by hexamethylenediamine and activated by glutaraldehyde before histidine was immobilized as affinity ligand. A membrane cartridge containing 40 sheets of affinity membrane was also prepared, which can be used to remove pyrogen from aqueous solutions. 展开更多
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Sensitive analysis of amino acids in injection liquor by reversed-phase HPLC
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作者 SHANG, ZH YU, YN +2 位作者 GUO, W JIANG, H zhou, lm 《Chinese Journal of Chemistry》 SCIE CAS CSCD 1995年第2期163-168,共6页
A convenient derivatization method of amino acids with 1-fluoro-2,4-dinitrobezene as reaction reagent and a separation system were described. The derivative amino acids were separated on a specific chemically bonded p... A convenient derivatization method of amino acids with 1-fluoro-2,4-dinitrobezene as reaction reagent and a separation system were described. The derivative amino acids were separated on a specific chemically bonded phase column with a simple linear gradient elution consisting of aqueous buffer and methanol. The eluate was detected by common ultraviolet absorption detector at 360 nm. The detection limits of amino acids were as low as 10 picomole. This method has been successfully applied to assay amino acid injection liquor used in hospital. It has good reproducibility and precision. The procedures avoid the requirements of particular derivative equipment and analyzer employed in conventional amino acid analysis. 展开更多
关键词 AMINO ACIDS PRECOLUMN DERIVATIZATION REVERSED PHASE SEPARATION UV ABSORPTION DETERMINATION
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