Alcohol consumption is one of the leading causes of death worldwide.Adolescence is a critical period of structural and functional maturation of the brain.Adolescent alcohol use can alter epigenetic modifications.Howev...Alcohol consumption is one of the leading causes of death worldwide.Adolescence is a critical period of structural and functional maturation of the brain.Adolescent alcohol use can alter epigenetic modifications.However,little is known on the long-term effects of alcohol consumption during adolescence on RNA epigenetic modifications in brain.Herein,we systematically explored the long-term effects of alcohol exposure during adolescence on small RNA modifications in adult rat brain tissues by comprehensive liquid chromatography-electrospray ionization-tandem mass spectrometry(LC-ESI-MS/MS)analysis.We totally detected 26 modifications in small RNA of brain tissues.Notably,we observed most of these modifications were decreased in brain tissues.These results suggest that alcohol exposure during adolescence may impose a long-lasting impact on RNA modifications in brain tissues.This is the first report that alcohol use during adolescence can alter RNA modifications in adult brain.Collectively,this study suggests a long-term adverse effects of alcohol consumption on brain from RNA epigenetics angle by comprehensive mass spectrometry analysis.展开更多
Alcohol consumption is a critical risk factor contributing to a verity of human diseases. The incidence of alcohol use disorder increases across adolescence in recent years. Accumulating line of evidence suggests that...Alcohol consumption is a critical risk factor contributing to a verity of human diseases. The incidence of alcohol use disorder increases across adolescence in recent years. Accumulating line of evidence suggests that alcohol-induced changes of DNA cytosine methylation(5-methyl-2-deoxycytidine, 5 m C) in genomes play an important role in the development of diseases. However, systemic investigation of the effects of adolescent alcohol exposure on DNA and RNA modifications is still lacked. Especially, there hasn’t been any report to study the effects of alcohol exposure on RNA modifications. Similar to DNA modifications,RNA modifications recently have been identified to function as new regulators in modulating numbers of biological processes. In the current study, we systematically investigated the effects of alcohol exposure on both DNA and RNA modifications in peripheral blood of adolescent rats by liquid chromatographyelectrospray ionization-tandem mass spectrometry(LC-ESI-MS/MS) analysis. The developed LC-ESI-MS/MS method enabled the sensitive and accurate determination of 2 DNA modifications and 12 RNA modifications. As for the alcohol exposure experiments, the adolescent rats were intraperitoneally injected with ethanol with an interval of one day for a total 14 days. The quantification results by LC-ESI-MS/MS analysis showed that adolescent alcohol exposure could alter both DNA and RNA modifications in peripheral blood. Specifically, we observed an overall decreased trend of RNA modifications. The discovery of the significant alteration of the levels of DNA and RNA modifications under alcohol exposure indicates that alcohol consumption may increase the risk of the incidence and development of diseases through dysregulating DNA and RNA modifications.展开更多
目的:研究基于院前预警系统开展的早期干预对缺血性脑卒中患者救治的影响。方法:入组由上海市奉贤区医疗急救中心接诊送往相应脑卒中中心的277例缺血性脑卒中患者,根据患者拨打120急救电话送入院前是否使用院前预警体系实施院前急救早...目的:研究基于院前预警系统开展的早期干预对缺血性脑卒中患者救治的影响。方法:入组由上海市奉贤区医疗急救中心接诊送往相应脑卒中中心的277例缺血性脑卒中患者,根据患者拨打120急救电话送入院前是否使用院前预警体系实施院前急救早期干预,将277例患者分为对照组(140例)和干预组(137例)。对2组的现场处置时间、入院至静脉溶栓治疗时间(door-to-needle time,DNT)、入院至股动脉穿刺时间(door to puncture time,DPT)、院前急救效果、并发症发生情况、预后情况进行比对。结果:干预组的现场处置时间、DNT、DPT时间均短于对照组,差异有统计学意义(P<0.001);干预组的转运成功率优于对照组,但差异不具统计学意义(P=0.574);干预组并发症总发生率低于对照组(P=0.036),预后情况优于对照组(P=0.028),差异均有统计学意义。结论:在应用院前预警系统的基础上开展院前急救早期干预可提升缺血性脑卒中患者急救效率,减少并发症的发生并改善患者预后。展开更多
基金the National Key R&D Program of China(Nos.2022YFA0806601,2022YFC3400700)the National Natural Science Foundation of China(Nos.22277093,22074110,21721005)+1 种基金the Interdisciplinary Innovative Talents Foundation from Renmin Hospital of Wuhan University(No.JCRCGW-2022-008)the Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University(No.ZNJC202208).
文摘Alcohol consumption is one of the leading causes of death worldwide.Adolescence is a critical period of structural and functional maturation of the brain.Adolescent alcohol use can alter epigenetic modifications.However,little is known on the long-term effects of alcohol consumption during adolescence on RNA epigenetic modifications in brain.Herein,we systematically explored the long-term effects of alcohol exposure during adolescence on small RNA modifications in adult rat brain tissues by comprehensive liquid chromatography-electrospray ionization-tandem mass spectrometry(LC-ESI-MS/MS)analysis.We totally detected 26 modifications in small RNA of brain tissues.Notably,we observed most of these modifications were decreased in brain tissues.These results suggest that alcohol exposure during adolescence may impose a long-lasting impact on RNA modifications in brain tissues.This is the first report that alcohol use during adolescence can alter RNA modifications in adult brain.Collectively,this study suggests a long-term adverse effects of alcohol consumption on brain from RNA epigenetics angle by comprehensive mass spectrometry analysis.
基金supported by the National Natural Science Foundation of China (Nos. 22074110, 21635006, 21721005, 31771193)the Fundamental Research Funds for the Central Universities (No.2042021kf0212)。
文摘Alcohol consumption is a critical risk factor contributing to a verity of human diseases. The incidence of alcohol use disorder increases across adolescence in recent years. Accumulating line of evidence suggests that alcohol-induced changes of DNA cytosine methylation(5-methyl-2-deoxycytidine, 5 m C) in genomes play an important role in the development of diseases. However, systemic investigation of the effects of adolescent alcohol exposure on DNA and RNA modifications is still lacked. Especially, there hasn’t been any report to study the effects of alcohol exposure on RNA modifications. Similar to DNA modifications,RNA modifications recently have been identified to function as new regulators in modulating numbers of biological processes. In the current study, we systematically investigated the effects of alcohol exposure on both DNA and RNA modifications in peripheral blood of adolescent rats by liquid chromatographyelectrospray ionization-tandem mass spectrometry(LC-ESI-MS/MS) analysis. The developed LC-ESI-MS/MS method enabled the sensitive and accurate determination of 2 DNA modifications and 12 RNA modifications. As for the alcohol exposure experiments, the adolescent rats were intraperitoneally injected with ethanol with an interval of one day for a total 14 days. The quantification results by LC-ESI-MS/MS analysis showed that adolescent alcohol exposure could alter both DNA and RNA modifications in peripheral blood. Specifically, we observed an overall decreased trend of RNA modifications. The discovery of the significant alteration of the levels of DNA and RNA modifications under alcohol exposure indicates that alcohol consumption may increase the risk of the incidence and development of diseases through dysregulating DNA and RNA modifications.
文摘目的:研究基于院前预警系统开展的早期干预对缺血性脑卒中患者救治的影响。方法:入组由上海市奉贤区医疗急救中心接诊送往相应脑卒中中心的277例缺血性脑卒中患者,根据患者拨打120急救电话送入院前是否使用院前预警体系实施院前急救早期干预,将277例患者分为对照组(140例)和干预组(137例)。对2组的现场处置时间、入院至静脉溶栓治疗时间(door-to-needle time,DNT)、入院至股动脉穿刺时间(door to puncture time,DPT)、院前急救效果、并发症发生情况、预后情况进行比对。结果:干预组的现场处置时间、DNT、DPT时间均短于对照组,差异有统计学意义(P<0.001);干预组的转运成功率优于对照组,但差异不具统计学意义(P=0.574);干预组并发症总发生率低于对照组(P=0.036),预后情况优于对照组(P=0.028),差异均有统计学意义。结论:在应用院前预警系统的基础上开展院前急救早期干预可提升缺血性脑卒中患者急救效率,减少并发症的发生并改善患者预后。
