To study the epidemic status of Ectromelia virus （ ECTV）, Mouse hepatitis virus （MHV） and Sendai virus （SV） in clean-grade experimental mice and rats in East China, 30 individuals of ICR and/or C57BL/6 mice and ...To study the epidemic status of Ectromelia virus （ ECTV）, Mouse hepatitis virus （MHV） and Sendai virus （SV） in clean-grade experimental mice and rats in East China, 30 individuals of ICR and/or C57BL/6 mice and 15 individuals of SD or Wistar rats were purchased from four major experimental animal supply bases in East China. Blood samples were collected by eyeball picking method, and serum was separated. ECTV, MHV and SV serum antibodies in mice were de- teeted using imported commercial ELISA kits. Since rats were not easily infected by MHV and SV, only the ECTV serum antibody was detected in rats. Survey re- suits revealed that clean-grade experimental mice and rats derived from three out of four major experimental animal supply bases were negative in detection, but the positive rates of MHV and SV serum antibody in clean-grade ICR mice in one supply base were 56.7% and 100.0%, respectively, while the positive rate of MHV serum antibody in C57BL/6 mice was 100%. Research results showed that most of the commercial experimental mice and rats in major experimental animal supply bases in East China were qualified in detection of above three kinds of diseases, but MHV and SV still existed in individual base, with severe infection status. Thus, active measures must be strengthened to purify mice and rats. Meanwhile, monitoring of mouse and rat diseases must be developed seriously to guarantee the quality of experimental mice and rats, so as to avoid adveme impacts on breeding and use of experimental rats and miee.展开更多
OBJECTIVE:To evaluate the therapeutic effectiveness of salidroside(Sal)and pyrrolidine dithiocarbamate(PDTC)against severe acute pancreatitis(SAP)in a rat model.METHODS:Rat models of SAP were established by retrograde...OBJECTIVE:To evaluate the therapeutic effectiveness of salidroside(Sal)and pyrrolidine dithiocarbamate(PDTC)against severe acute pancreatitis(SAP)in a rat model.METHODS:Rat models of SAP were established by retrograde infusion of sodium taurocholate solution.SAP rats were randomly divided into 6 groups:SAP 3 h group,SAP 24 h group,low-dose Sal treatment group(Sal L+S),middle-dose Sal treatment group(Sal M+S),high-dose Sal treatment group(Sal H+S)and PDTC treatment group(PDTC+S).The serum amylase,tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-10(IL-10)levels were determined by optical turbidimetry and enzyme-linked immunosorbent assay.The expression of Beclin-1,microtubule-associated protein light chain 3Ⅱ(LC3Ⅱ),lysosome associated membrane protein 2(LAMP2),interleukin-1 receptor associated kinase 1(IRAK1),inhibitorαof nuclear transcription factor-κB(IκBα),nuclear transcription factor-κB 65(p65)in the pancreas tissues were detected by quantitative real-time polymerase chain reaction and Western blot,while the pIκBαand p-p65 levels were detected by Western blot.Pathological changes of the pancreas and all the other indexes were observed at 3 and 24 h after operation.RESULTS:The serum IL-10 level,IκBαand LAMP2 levels in Sal M+S,Sal H+S and PDTC+S groups were higher than those in SAP 24 h group,while all the other indexes in these three groups were all lower significantly than those in SAP 24 h group.There was no significant difference in all indexes between Sal H+S and PDTC+S groups.CONCLUSION:High-dose Sal has an effectively therapeutic effect on SAP in rats,which was similar to PDTC.展开更多
基金Supported by Natural Science Foundation of Jiangsu Province(BK2010279)Science and Technology Program of Jiangsu Entry-exit Inspection and Quarantine Bureau(2013KJ03)Natural Science Research Project of Colleges and Universities in Jiangsu Province(13KJB180019)
文摘To study the epidemic status of Ectromelia virus （ ECTV）, Mouse hepatitis virus （MHV） and Sendai virus （SV） in clean-grade experimental mice and rats in East China, 30 individuals of ICR and/or C57BL/6 mice and 15 individuals of SD or Wistar rats were purchased from four major experimental animal supply bases in East China. Blood samples were collected by eyeball picking method, and serum was separated. ECTV, MHV and SV serum antibodies in mice were de- teeted using imported commercial ELISA kits. Since rats were not easily infected by MHV and SV, only the ECTV serum antibody was detected in rats. Survey re- suits revealed that clean-grade experimental mice and rats derived from three out of four major experimental animal supply bases were negative in detection, but the positive rates of MHV and SV serum antibody in clean-grade ICR mice in one supply base were 56.7% and 100.0%, respectively, while the positive rate of MHV serum antibody in C57BL/6 mice was 100%. Research results showed that most of the commercial experimental mice and rats in major experimental animal supply bases in East China were qualified in detection of above three kinds of diseases, but MHV and SV still existed in individual base, with severe infection status. Thus, active measures must be strengthened to purify mice and rats. Meanwhile, monitoring of mouse and rat diseases must be developed seriously to guarantee the quality of experimental mice and rats, so as to avoid adveme impacts on breeding and use of experimental rats and miee.
基金Supported by the Traditional Chinese Medicine Technology Development Plan Project of Jiangsu Province in 2020:Basic and Clinical Study on Salidroside in the Adjuvant Treatment of Severe Acute Pancreatitis(No.YB2020088)the Health Innovation Project of Lvyang Jinfeng Plan of Yangzhou City in 2020:Basic and Clinical Study on Salidroside in the Adjuvant Treatment of Severe Acute Pancreatitis(No.LJ202037)。
文摘OBJECTIVE:To evaluate the therapeutic effectiveness of salidroside(Sal)and pyrrolidine dithiocarbamate(PDTC)against severe acute pancreatitis(SAP)in a rat model.METHODS:Rat models of SAP were established by retrograde infusion of sodium taurocholate solution.SAP rats were randomly divided into 6 groups:SAP 3 h group,SAP 24 h group,low-dose Sal treatment group(Sal L+S),middle-dose Sal treatment group(Sal M+S),high-dose Sal treatment group(Sal H+S)and PDTC treatment group(PDTC+S).The serum amylase,tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-10(IL-10)levels were determined by optical turbidimetry and enzyme-linked immunosorbent assay.The expression of Beclin-1,microtubule-associated protein light chain 3Ⅱ(LC3Ⅱ),lysosome associated membrane protein 2(LAMP2),interleukin-1 receptor associated kinase 1(IRAK1),inhibitorαof nuclear transcription factor-κB(IκBα),nuclear transcription factor-κB 65(p65)in the pancreas tissues were detected by quantitative real-time polymerase chain reaction and Western blot,while the pIκBαand p-p65 levels were detected by Western blot.Pathological changes of the pancreas and all the other indexes were observed at 3 and 24 h after operation.RESULTS:The serum IL-10 level,IκBαand LAMP2 levels in Sal M+S,Sal H+S and PDTC+S groups were higher than those in SAP 24 h group,while all the other indexes in these three groups were all lower significantly than those in SAP 24 h group.There was no significant difference in all indexes between Sal H+S and PDTC+S groups.CONCLUSION:High-dose Sal has an effectively therapeutic effect on SAP in rats,which was similar to PDTC.
