Background Aphis gossypii(Hemiptera:Aphididae)is a worldwide polyphagous phloem-feeding agricultural pest,and it can produce offspring by sexual or asexual reproduction.Compared with dozens of generations by parthenog...Background Aphis gossypii(Hemiptera:Aphididae)is a worldwide polyphagous phloem-feeding agricultural pest,and it can produce offspring by sexual or asexual reproduction.Compared with dozens of generations by parthenogenesis,sexual reproduction is performed in only one generation within one year,and little is known about the sexual reproduction of A.gossypii.In this study,sexual females of A.gossypii were successfully obtained through a previously established induction platform,and the morphological characteristics,developmental dynamics,and temporal gene expression were examined.Subsequently,signaling pathways potentially involved in regulating the growth,development,and reproduction of sexual females were investigated.Results The morphological observation showed that from the 1st instar nymph to adult,sexual females exhibited a gradually deepened body color,an enlarged body size,longer antennae with a blackened end,and obviously protruding cauda(in adulthood).The anatomy found that the ovaries of sexual females developed rapidly from the 2^(nd)instar nymph,and the embedded oocytes matured in adulthood.In addition,time-course transcriptome analysis revealed that gene expression profiles across the development of sexual females fell into 9 clusters with distinct patterns,in which gene expression levels in clusters 1,5,and 8 peaked at the 2^(nd)instar nymphal stage with the largest number of up-regulated genes,suggesting that the 2^(nd)instar nymph was an important ovary development period.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis revealed that a large number of genes in the sexual female adult were enriched in the TGF-beta signaling pathway and Forkhead box O(FoxO)signaling pathway,highlighting their important role in sexual female adult development and reproduction.Conclusion The morphological changes of the sexual female at each developmental stage were revealed for the first time.In addition,time-course transcriptomic analyses suggest genes enriched in the TGF-beta signaling pathway and FoxO signaling pathway probably contribute to regulating the development and oocyte maturation of sexual females.Overall,these findings will facilitate the regulating mechanism research in the growth and development of sexual females by providing candidate genes.展开更多
共生菌和昆虫是互利共生的关系,它参与宿主生殖、代谢、解毒等生理过程。龟纹瓢虫为农田生态系统重要天敌昆虫,同时具有取食植物花粉的习性。为明确龟纹瓢虫体内共生菌的种类与群落结构,通过Illumina MiSeq平台对龟纹瓢虫幼虫体内共生菌...共生菌和昆虫是互利共生的关系,它参与宿主生殖、代谢、解毒等生理过程。龟纹瓢虫为农田生态系统重要天敌昆虫,同时具有取食植物花粉的习性。为明确龟纹瓢虫体内共生菌的种类与群落结构,通过Illumina MiSeq平台对龟纹瓢虫幼虫体内共生菌的16S r DNA V3~V4区进行高通量测序,生物信息学方法分析共生菌的种类和多样性。结果显示,共获得3910个OTUs,长度为360~480 bp的序列占99.54%;OTU注释结果表明,龟纹瓢虫共生菌包括37个门,75个纲,143个目,261个科,605个属;变形菌门Proteobacteria(51.84%)、厚壁菌门Firmicutes(21.81%)、放线菌门Actinobacteria(11.35%)、蓝藻菌门Cyanobacteria(7.06%)、拟杆菌门Bacteroidetes(4.49%)为龟纹瓢虫幼虫的5个优势种群。在属水平上葡萄球菌Staphylococcus(16%)、不动杆菌属Acinetobacter(8.54%)、Paracocccus(4.93%)、假单胞菌Pseudomonas(3.59%)、Boechera gunnisoniana(3.34%)、棒状杆菌属Corynebacterium(2.18%)、阪崎肠杆菌Cronobacter(2.04%)、考克氏菌属Kocuria(1.50%)、链球菌属Streptococcus(1.15%)和芽胞杆菌Bacillus(1.11%)为优势菌群。本研究初步明确了龟纹瓢虫幼虫体内共生菌种群组成与结构,为通过共生菌提高龟纹瓢虫人工繁殖效率提供参考,同时为转基因植物对龟纹瓢虫的安全评价提供新的思路。展开更多
【目的】旨在明确不同翅型棉蚜体内细菌差异,探究体内细菌与棉蚜翅型分化间的关系。【方法】通过Illumina Mi Seq高通量测序分别对有翅型和无翅型棉蚜体内细菌16S r RNA基因V3~V4区进行测序,并根据测序结果进行操作分类单元(operational...【目的】旨在明确不同翅型棉蚜体内细菌差异,探究体内细菌与棉蚜翅型分化间的关系。【方法】通过Illumina Mi Seq高通量测序分别对有翅型和无翅型棉蚜体内细菌16S r RNA基因V3~V4区进行测序,并根据测序结果进行操作分类单元(operational taxonomic unit, OTU)聚类分析、α多样性分析、菌种组成与分类等分析。【结果】在所有棉蚜样本中共鉴定出234个体内细菌OTU,隶属于16门、32纲、64目、107科、148属、187种,其中布赫纳氏菌属(Buchnera)是优势菌属。2种翅型棉蚜体内细菌多样性不同:无翅型(隶属于12门、23纲、50目、82科、106属、136种)略高于有翅型(隶属于14门、26纲、45目、72科、97属、120种)。多种体内细菌的相对丰度在2种翅型棉蚜中存在显著差异。其中:红球菌属(Rhodococcus)细菌在有翅型棉蚜中的相对丰度较高(0.136%),而在无翅型棉蚜中较低(0.078%);未分类的蓝藻纲细菌在有翅型棉蚜体内相对丰度较低(0.005%),而在无翅型棉蚜中较高(0.024%)。【结论】有翅型和无翅型棉蚜的体内细菌组成和群落结构存在一定的差异。该研究拓展了对棉蚜翅二型现象的认知。展开更多
基金funded by National Natural Science Foundation of China(No.32102214)Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences+1 种基金China Agriculture Research System(CARS-15-21)National Key R&D Program of China(2022YFD1400300)。
文摘Background Aphis gossypii(Hemiptera:Aphididae)is a worldwide polyphagous phloem-feeding agricultural pest,and it can produce offspring by sexual or asexual reproduction.Compared with dozens of generations by parthenogenesis,sexual reproduction is performed in only one generation within one year,and little is known about the sexual reproduction of A.gossypii.In this study,sexual females of A.gossypii were successfully obtained through a previously established induction platform,and the morphological characteristics,developmental dynamics,and temporal gene expression were examined.Subsequently,signaling pathways potentially involved in regulating the growth,development,and reproduction of sexual females were investigated.Results The morphological observation showed that from the 1st instar nymph to adult,sexual females exhibited a gradually deepened body color,an enlarged body size,longer antennae with a blackened end,and obviously protruding cauda(in adulthood).The anatomy found that the ovaries of sexual females developed rapidly from the 2^(nd)instar nymph,and the embedded oocytes matured in adulthood.In addition,time-course transcriptome analysis revealed that gene expression profiles across the development of sexual females fell into 9 clusters with distinct patterns,in which gene expression levels in clusters 1,5,and 8 peaked at the 2^(nd)instar nymphal stage with the largest number of up-regulated genes,suggesting that the 2^(nd)instar nymph was an important ovary development period.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis revealed that a large number of genes in the sexual female adult were enriched in the TGF-beta signaling pathway and Forkhead box O(FoxO)signaling pathway,highlighting their important role in sexual female adult development and reproduction.Conclusion The morphological changes of the sexual female at each developmental stage were revealed for the first time.In addition,time-course transcriptomic analyses suggest genes enriched in the TGF-beta signaling pathway and FoxO signaling pathway probably contribute to regulating the development and oocyte maturation of sexual females.Overall,these findings will facilitate the regulating mechanism research in the growth and development of sexual females by providing candidate genes.
文摘共生菌和昆虫是互利共生的关系,它参与宿主生殖、代谢、解毒等生理过程。龟纹瓢虫为农田生态系统重要天敌昆虫,同时具有取食植物花粉的习性。为明确龟纹瓢虫体内共生菌的种类与群落结构,通过Illumina MiSeq平台对龟纹瓢虫幼虫体内共生菌的16S r DNA V3~V4区进行高通量测序,生物信息学方法分析共生菌的种类和多样性。结果显示,共获得3910个OTUs,长度为360~480 bp的序列占99.54%;OTU注释结果表明,龟纹瓢虫共生菌包括37个门,75个纲,143个目,261个科,605个属;变形菌门Proteobacteria(51.84%)、厚壁菌门Firmicutes(21.81%)、放线菌门Actinobacteria(11.35%)、蓝藻菌门Cyanobacteria(7.06%)、拟杆菌门Bacteroidetes(4.49%)为龟纹瓢虫幼虫的5个优势种群。在属水平上葡萄球菌Staphylococcus(16%)、不动杆菌属Acinetobacter(8.54%)、Paracocccus(4.93%)、假单胞菌Pseudomonas(3.59%)、Boechera gunnisoniana(3.34%)、棒状杆菌属Corynebacterium(2.18%)、阪崎肠杆菌Cronobacter(2.04%)、考克氏菌属Kocuria(1.50%)、链球菌属Streptococcus(1.15%)和芽胞杆菌Bacillus(1.11%)为优势菌群。本研究初步明确了龟纹瓢虫幼虫体内共生菌种群组成与结构,为通过共生菌提高龟纹瓢虫人工繁殖效率提供参考,同时为转基因植物对龟纹瓢虫的安全评价提供新的思路。
文摘【目的】旨在明确不同翅型棉蚜体内细菌差异,探究体内细菌与棉蚜翅型分化间的关系。【方法】通过Illumina Mi Seq高通量测序分别对有翅型和无翅型棉蚜体内细菌16S r RNA基因V3~V4区进行测序,并根据测序结果进行操作分类单元(operational taxonomic unit, OTU)聚类分析、α多样性分析、菌种组成与分类等分析。【结果】在所有棉蚜样本中共鉴定出234个体内细菌OTU,隶属于16门、32纲、64目、107科、148属、187种,其中布赫纳氏菌属(Buchnera)是优势菌属。2种翅型棉蚜体内细菌多样性不同:无翅型(隶属于12门、23纲、50目、82科、106属、136种)略高于有翅型(隶属于14门、26纲、45目、72科、97属、120种)。多种体内细菌的相对丰度在2种翅型棉蚜中存在显著差异。其中:红球菌属(Rhodococcus)细菌在有翅型棉蚜中的相对丰度较高(0.136%),而在无翅型棉蚜中较低(0.078%);未分类的蓝藻纲细菌在有翅型棉蚜体内相对丰度较低(0.005%),而在无翅型棉蚜中较高(0.024%)。【结论】有翅型和无翅型棉蚜的体内细菌组成和群落结构存在一定的差异。该研究拓展了对棉蚜翅二型现象的认知。
