Three-dimensional(3D)bioprinting has been used widely for the construction of hard tissues such as bone and cartilage.However,constructing soft tissues with complex structures remains a challenge.In this study,complex...Three-dimensional(3D)bioprinting has been used widely for the construction of hard tissues such as bone and cartilage.However,constructing soft tissues with complex structures remains a challenge.In this study,complex structures characterized by both tunable elastic modulus and porosity were printed using freeform reversible embedding of suspended hydrogels(FRESHs)printing methods.A mixture of alginate and gelatin was used as the main functional component of the bioink.Rheological analysis showed that this bioink possesses shear thinning and shear recovery properties,supporting both cryogenic and FRESH printing methods.Potential printing capabilities and limitations of cryogenic and FRESH printing were then analyzed by printability tests.A series of complex structures were printed by FRESH printing methods which could not be realized using conventional approaches.Mechanical tests and scanning electron microscopy analysis showed that the printed structure is of excellent flexibility and could be applied in various conditions by adjusting its mechanical modulus and porosity.L929 fibroblast cells maintained cell viability in cell-laden-printed structures,and the addition of collagen further improved the hydrogels’biocompatibility.Overall,all results provided useful insight into the building of human soft tissue organ blocks.展开更多
With the increasing use of difficult-to-machine materials in aerospace applications,machining requirements are becoming ever more rigorous.However,traditional single-point diamond turning(SPDT)can cause surface damage...With the increasing use of difficult-to-machine materials in aerospace applications,machining requirements are becoming ever more rigorous.However,traditional single-point diamond turning(SPDT)can cause surface damage and tool wear.Thus,it is difficult for SPDT to meet the processing requirements,and it has significant limitations.Research indicates that supplementing SPDT with unconventional techniques can,importantly,solve problems due to the high cutting forces and poor surface quality for difficult-to-machine materials.This paper first introduces SPDT and reviews research into unconventional techniques for use with SPDT.The machining mechanism is discussed,and the main advantages and disadvantages of various methods are investigated.Second,hybrid SPDT is briefly described,which encompasses ultrasonic-vibration magnetic-field SPDT,ultrasonic-vibration laser SPDT,and ultrasonic-vibration cold-plasma SPDT.Compared with the traditional SPDT method,hybrid SPDT produces a better optical surface quality.The current status of research into unconventional techniques to supplement SPDT is then summarized.Finally,future development trends and the application prospects of unconventional assisted SPDT are discussed.展开更多
The present study was conducted to determine effects of different forms of yeast （Saccharomyces cerevisiae, strain Y200007） on the growth performance, intestinal development, and systemic immunity in early-weaned pi...The present study was conducted to determine effects of different forms of yeast （Saccharomyces cerevisiae, strain Y200007） on the growth performance, intestinal development, and systemic immunity in early-weaned piglets. A total of 96 piglets （14-d old, initial average body weight of 4.5 kg） were assigned to 4 dietary treatments： （1） basa diet without yeast （Control）; （2） basal diet supplemented with 3.00 g/kg live yeast （LY）; （3） basal diet supplemented with 2.66 g/kg heat-killed whole yeast （HKY）; and （4） basal diet supplemented with 3.00 g/kg superfine yeast powders （SFY）. Diets and water were provided ad libitum to the piglets during 3-week experiment. Growth performance of piglets was measured weekly. Samples of blood and small intestine were collected at days 7 and 21 of experiment. Dietary supplementation with LY and SFY improved G：F of piglets at days ]-21 of the experiment （P 〈 0.05） compared to Control group. Serum concentrations of growth hormone （GH）, triiodothyronine （T3）, tetraiodothyronine （T4）, and insulin growth factor 1 （iGF-1） in piglets at day 21 of the experiment were higher when fed diets supplemented with LY and SFY than those in Control group （P 〈 0.05）. Compared to Control group, contents of serum urea nitrogen of piglets were reduced by the 3 yeast-supplemented diets （P 〈 0.05）. Diets supplemented with LY increased villus height and villus-to-crypt ratio in duodenum and jejunum of piglets （P 〈 0.05） compared to other two groups at day 7 of the experiment. Feeding diets supplemented with LY and SFY increased （P 〈 0.05） serum concentrations of IgA, IL-2, and IL-6 levels in piglets compared to Control. The CD4＋/CD8＋ ratio and proliferation of T-lymphocytes in piglets fed diets supplemented with LY were increased compared to that of Control group at day 7 of the experiment （P 〈 0.05）. In conclusion, dietary supplementation with both LY and SFY enhanced feed conversion, small intestinal development, and systemic immunity in early-weaned piglets, with better improvement in feed conversion by dietary supplementation with LY, while dietary supplementation with SFY was more effective in increasing systemic immune functions in early-weaned piglets.展开更多
In this study,a peridynamic fiber-reinforced concrete model is developed based on the bond-based peridynamic model with rotation effect(BBPDR).The fibers are modelled by a semi-discrete method and distributed with ran...In this study,a peridynamic fiber-reinforced concrete model is developed based on the bond-based peridynamic model with rotation effect(BBPDR).The fibers are modelled by a semi-discrete method and distributed with random locations and angles in the concrete specimen,since the fiber content is low,and its scale is smaller than the concrete matrix.The interactions between fibers and concrete matrix are investigated by the improvement of the bond’s strength and stiffness.Also,the frictional effect between the fibers and the concrete matrix is considered,which is divided into static friction and slip friction.To validate the proposed model,several examples are simulated,including the tensile test and the three-point bending beam test.And the numerical results of the proposed model are compared with the experiments and other numerical models.The comparisons show that the proposed model is capable of simulating the fracture behavior of the fiber-reinforced concrete.After adding the fibers,the tensile strength,bending strength,and toughness of the fiber-reinforced concrete specimens are improved.Besides,the fibers distribution has an impact on the crack path,especially in the three-point bending beam test.展开更多
The peridynamic correspondence model provides a general formulation to incorporate the classical local model and,therefore,helps to solve mechanical problems with discontinuities easily.But it suffers from zero-energy...The peridynamic correspondence model provides a general formulation to incorporate the classical local model and,therefore,helps to solve mechanical problems with discontinuities easily.But it suffers from zero-energy mode instability in numerical implementation due to the approximation of deformation gradient tensor.To suppress zero-energy modes,previous stabilized methods were generally more based on adding a supplemental force state derived from bond-based peridynamic theory,which requires a bond-based peridynamic micro-modulus.In this work,we present an improved stabilized method where the stabilization force state is derived directly from the peridynamic correspondence model.Hence,the bond-based peridynamic micro-modulus is abandoned.This improved method needs no extra constant to control the magnitude of stabilization force state and it is suitable for either isotropic or anisotropic materials.Several examples are presented to demonstrate its performance in simulating crack propagation,and numerical results show its efficiency and effectiveness.展开更多
As one of the biological endogenous pigments,biliverdin(BV)and its dimethyl ester(BVE)have extremely weak uorescence in solution with quantum yield less than 0.01%.However,the situation reverses with the addition of z...As one of the biological endogenous pigments,biliverdin(BV)and its dimethyl ester(BVE)have extremely weak uorescence in solution with quantum yield less than 0.01%.However,the situation reverses with the addition of zinc ions.The strength for uorescence of BVE-Zn^2+ complex is greatly enhanced and uorescence quantum yield can increase to5%.Herein,we studied ultrafast excited state dynamics of BVE-Zn^2+ complex in ethanol,npropanol,and DMSO solutions in order to reveal the mechanism of uorescence quantum yield enhancement.The results show that BVE can form a stable coordination complex with zinc with 1:1 stoichiometry in solution.BVE is structurally and energetically more stable in the complex.Using picosecond time-resolve uorescence and femtosecond transient absorption spectroscopy,we show that smaller non-radiative rate constant of BVE-Zn^2+ complex in DMSO is the key to increasing its uorescence quantum yield and the excited state decay mechanism is also revealed.These results provide valuable information about the uorescence property change after BVE binding to metal ions and may provide a guidance for the study of phytochromes or other uorescence proteins in which BV/BVE acts as chromophores.展开更多
基金supported by the National Natural Science Foundation of China(Nos.52275464 and 52075300)the Scientific Research Project for National High-Level Innovative Talents ofHebei Province Full-Time Introduction(No.2021HBQZYCXY004).
文摘Three-dimensional(3D)bioprinting has been used widely for the construction of hard tissues such as bone and cartilage.However,constructing soft tissues with complex structures remains a challenge.In this study,complex structures characterized by both tunable elastic modulus and porosity were printed using freeform reversible embedding of suspended hydrogels(FRESHs)printing methods.A mixture of alginate and gelatin was used as the main functional component of the bioink.Rheological analysis showed that this bioink possesses shear thinning and shear recovery properties,supporting both cryogenic and FRESH printing methods.Potential printing capabilities and limitations of cryogenic and FRESH printing were then analyzed by printability tests.A series of complex structures were printed by FRESH printing methods which could not be realized using conventional approaches.Mechanical tests and scanning electron microscopy analysis showed that the printed structure is of excellent flexibility and could be applied in various conditions by adjusting its mechanical modulus and porosity.L929 fibroblast cells maintained cell viability in cell-laden-printed structures,and the addition of collagen further improved the hydrogels’biocompatibility.Overall,all results provided useful insight into the building of human soft tissue organ blocks.
基金supported by the National Natural Science Foundation of China(Grant No.52175431)the Natural Science Foundation of Tianjin of China(Grant No.22JCZDJC00730)the Scientific Research Project of Tianjin Municipal Education Commission(Grant No.2022ZD021).
文摘With the increasing use of difficult-to-machine materials in aerospace applications,machining requirements are becoming ever more rigorous.However,traditional single-point diamond turning(SPDT)can cause surface damage and tool wear.Thus,it is difficult for SPDT to meet the processing requirements,and it has significant limitations.Research indicates that supplementing SPDT with unconventional techniques can,importantly,solve problems due to the high cutting forces and poor surface quality for difficult-to-machine materials.This paper first introduces SPDT and reviews research into unconventional techniques for use with SPDT.The machining mechanism is discussed,and the main advantages and disadvantages of various methods are investigated.Second,hybrid SPDT is briefly described,which encompasses ultrasonic-vibration magnetic-field SPDT,ultrasonic-vibration laser SPDT,and ultrasonic-vibration cold-plasma SPDT.Compared with the traditional SPDT method,hybrid SPDT produces a better optical surface quality.The current status of research into unconventional techniques to supplement SPDT is then summarized.Finally,future development trends and the application prospects of unconventional assisted SPDT are discussed.
基金financially supported by grants from China Agriculture Research System(CARS-36)the Special Fund for Agro-scientific Research in the Public Interest(No.201403047)+1 种基金National Basic Research Program of China(2013CB127301 and 2013CB127304)Presidential Foundation of Guangdong Academy of Agricultural Sciences(201312)
文摘The present study was conducted to determine effects of different forms of yeast （Saccharomyces cerevisiae, strain Y200007） on the growth performance, intestinal development, and systemic immunity in early-weaned piglets. A total of 96 piglets （14-d old, initial average body weight of 4.5 kg） were assigned to 4 dietary treatments： （1） basa diet without yeast （Control）; （2） basal diet supplemented with 3.00 g/kg live yeast （LY）; （3） basal diet supplemented with 2.66 g/kg heat-killed whole yeast （HKY）; and （4） basal diet supplemented with 3.00 g/kg superfine yeast powders （SFY）. Diets and water were provided ad libitum to the piglets during 3-week experiment. Growth performance of piglets was measured weekly. Samples of blood and small intestine were collected at days 7 and 21 of experiment. Dietary supplementation with LY and SFY improved G：F of piglets at days ]-21 of the experiment （P 〈 0.05） compared to Control group. Serum concentrations of growth hormone （GH）, triiodothyronine （T3）, tetraiodothyronine （T4）, and insulin growth factor 1 （iGF-1） in piglets at day 21 of the experiment were higher when fed diets supplemented with LY and SFY than those in Control group （P 〈 0.05）. Compared to Control group, contents of serum urea nitrogen of piglets were reduced by the 3 yeast-supplemented diets （P 〈 0.05）. Diets supplemented with LY increased villus height and villus-to-crypt ratio in duodenum and jejunum of piglets （P 〈 0.05） compared to other two groups at day 7 of the experiment. Feeding diets supplemented with LY and SFY increased （P 〈 0.05） serum concentrations of IgA, IL-2, and IL-6 levels in piglets compared to Control. The CD4＋/CD8＋ ratio and proliferation of T-lymphocytes in piglets fed diets supplemented with LY were increased compared to that of Control group at day 7 of the experiment （P 〈 0.05）. In conclusion, dietary supplementation with both LY and SFY enhanced feed conversion, small intestinal development, and systemic immunity in early-weaned piglets, with better improvement in feed conversion by dietary supplementation with LY, while dietary supplementation with SFY was more effective in increasing systemic immune functions in early-weaned piglets.
基金The authors are pleased to acknowledge the support by the National Natural Science Foundation of China through contract/Grant Nos.11772237,11472196 and 11172216to acknowledge the open funds of the State Key Laboratory of Structural Analysis for Industrial Equipment(Dalian University of Technology)through contract/Grant No.GZ19110.
文摘In this study,a peridynamic fiber-reinforced concrete model is developed based on the bond-based peridynamic model with rotation effect(BBPDR).The fibers are modelled by a semi-discrete method and distributed with random locations and angles in the concrete specimen,since the fiber content is low,and its scale is smaller than the concrete matrix.The interactions between fibers and concrete matrix are investigated by the improvement of the bond’s strength and stiffness.Also,the frictional effect between the fibers and the concrete matrix is considered,which is divided into static friction and slip friction.To validate the proposed model,several examples are simulated,including the tensile test and the three-point bending beam test.And the numerical results of the proposed model are compared with the experiments and other numerical models.The comparisons show that the proposed model is capable of simulating the fracture behavior of the fiber-reinforced concrete.After adding the fibers,the tensile strength,bending strength,and toughness of the fiber-reinforced concrete specimens are improved.Besides,the fibers distribution has an impact on the crack path,especially in the three-point bending beam test.
基金This work was supported by the National Natural Science Foundation of China(Grants 11472196,11172216 and 11772237).
文摘The peridynamic correspondence model provides a general formulation to incorporate the classical local model and,therefore,helps to solve mechanical problems with discontinuities easily.But it suffers from zero-energy mode instability in numerical implementation due to the approximation of deformation gradient tensor.To suppress zero-energy modes,previous stabilized methods were generally more based on adding a supplemental force state derived from bond-based peridynamic theory,which requires a bond-based peridynamic micro-modulus.In this work,we present an improved stabilized method where the stabilization force state is derived directly from the peridynamic correspondence model.Hence,the bond-based peridynamic micro-modulus is abandoned.This improved method needs no extra constant to control the magnitude of stabilization force state and it is suitable for either isotropic or anisotropic materials.Several examples are presented to demonstrate its performance in simulating crack propagation,and numerical results show its efficiency and effectiveness.
基金the National Nature Science Foundation of China(No.11674101,No.21873030 and No.91850202)。
文摘As one of the biological endogenous pigments,biliverdin(BV)and its dimethyl ester(BVE)have extremely weak uorescence in solution with quantum yield less than 0.01%.However,the situation reverses with the addition of zinc ions.The strength for uorescence of BVE-Zn^2+ complex is greatly enhanced and uorescence quantum yield can increase to5%.Herein,we studied ultrafast excited state dynamics of BVE-Zn^2+ complex in ethanol,npropanol,and DMSO solutions in order to reveal the mechanism of uorescence quantum yield enhancement.The results show that BVE can form a stable coordination complex with zinc with 1:1 stoichiometry in solution.BVE is structurally and energetically more stable in the complex.Using picosecond time-resolve uorescence and femtosecond transient absorption spectroscopy,we show that smaller non-radiative rate constant of BVE-Zn^2+ complex in DMSO is the key to increasing its uorescence quantum yield and the excited state decay mechanism is also revealed.These results provide valuable information about the uorescence property change after BVE binding to metal ions and may provide a guidance for the study of phytochromes or other uorescence proteins in which BV/BVE acts as chromophores.
