Autopolyploidy and allopolyploidy may represent an evolutionary advantage and are more common in plants than assumed. However, less attention has been paid to autopolyploidy than to allopolyploidy,and its evolutionary...Autopolyploidy and allopolyploidy may represent an evolutionary advantage and are more common in plants than assumed. However, less attention has been paid to autopolyploidy than to allopolyploidy,and its evolutionary consequences are largely unclear, especially for plants with high ploidy levels. In this study, we developed oligonucleotide(oligo)-based chromosome painting probes to identify individual chromosomes in S. spontaneum. Using fluorescence in situ hybridization(FISH), we investigated chromosome behavior during pachytene, metaphase, anaphase, and telophase of meiosis I(MI) in autotetraploid,autooctoploid, and autodecaploid S. spontaneum clones. All autopolyploid clones showed stable diploidized chromosome behavior;so that homologous chromosomes formed almost exclusively bivalents during MI. Two copies of homologous chromosome 8 with similar sizes in the autotetraploid clone showed preferential pairing with each other with respect to the other copies. However, sequence variation analysis showed no apparent differences among homologs of chromosome 8 and all other chromosomes. We suggest that either the stable diploidized pairing or the preferential pairing between homologous copies of chromosome 8 in the studied autopolyploid sugarcane are accounted for by unknown mechanisms other than DNA sequence similarity. Our results reveal evolutionary consequences of stable meiotic behavior in autopolyploid plants.展开更多
目的结直肠癌是全球常见的恶性肿瘤之一,经自然腔道取标本手术(NOSES)作为一种微创手术,近年来在结直肠癌的治疗中得到了广泛的应用.然而,对于NOSES手术的国际研究现状尚未有系统的文献计量学分析.方法本研究利用Web of Science Core Co...目的结直肠癌是全球常见的恶性肿瘤之一,经自然腔道取标本手术(NOSES)作为一种微创手术,近年来在结直肠癌的治疗中得到了广泛的应用.然而,对于NOSES手术的国际研究现状尚未有系统的文献计量学分析.方法本研究利用Web of Science Core Collection数据库,对2000年至2023年间发表的关于结直肠肿瘤NOSES手术的文章进行检索,使用Bibliometrix文献计量学方法,通过对国家、机构、期刊、作者和关键词进行分析.结果本研究共纳入了316篇文献,其中2022年的最高发文量达到了44篇.中国在该领域中扮演着主要的贡献者角色,尤其是中国医学科学院&北京协和医学院,其发表的文献数量高达50篇.此外,美国的期刊《Surgical Endoscopy and Other Interventional Techniques》刊文量最多.通过聚类分析,发现研究热点仍然是NOSES技术在结直肠癌手术的应用,而中国仍然是NOSES研究的主要中心.结论本研究系统总结了2000~2023年结直肠肿瘤NOSES手术的研究成果,对全球范围内的研究趋势进行了描述,中国在引领该领域研究的发展中发挥着重要的作用.NOSES手术的技术进步和临床应用将是未来研究的重点.展开更多
Crop wild relatives are an important reservoir of natural biodiversity. However, incorporating wild geneticdiversity into breeding programs is often hampered by reproductive barriers and a lack of accurate genomicinfo...Crop wild relatives are an important reservoir of natural biodiversity. However, incorporating wild geneticdiversity into breeding programs is often hampered by reproductive barriers and a lack of accurate genomicinformation. We assembled a high-quality, accurately centromere-anchored genome of Gossypium anomalum, a stress-tolerant wild cotton species. We provided a strategy to discover and transfer agronomicallyvaluable genes from wild diploid species to tetraploid cotton cultivars. With a (Gossypium hirsutum 3 G.anomalum)2 hexaploid as a bridge parent, we developed a set of 74 diploid chromosome segment substitution lines (CSSLs) of the wild cotton species G. anomalum in the G. hirsutum background. This set of CSSLsincluded 70 homozygous substitutions and four heterozygous substitutions, and it collectively containedabout 72.22% of the G. anomalum genome. Twenty-four quantitative trait loci associated with plant height,yield, and fiber qualities were detected on 15 substitution segments. Integrating the reference genome withagronomic trait evaluation of the CSSLs enabled location and cloning of two G. anomalum genes thatencode peroxiredoxin and putative callose synthase 8, respectively, conferring drought tolerance andimproving fiber strength. We have demonstrated the power of a high-quality wild-species reference genomefor identifying agronomically valuable alleles to facilitate interspecific introgression breeding in crops.展开更多
基金funded by the Startup Foundation from Nantong University (03083074)partially supported by the National Natural Science Foundation of China (31771862)+1 种基金Special Funds for Technology Innovation of Fujian Agriculture and Forestry University(KFA20001A)the Research Program of Guangxi Key Laboratory for Sugarcane Biology (GXKLSCB-20190203)。
文摘Autopolyploidy and allopolyploidy may represent an evolutionary advantage and are more common in plants than assumed. However, less attention has been paid to autopolyploidy than to allopolyploidy,and its evolutionary consequences are largely unclear, especially for plants with high ploidy levels. In this study, we developed oligonucleotide(oligo)-based chromosome painting probes to identify individual chromosomes in S. spontaneum. Using fluorescence in situ hybridization(FISH), we investigated chromosome behavior during pachytene, metaphase, anaphase, and telophase of meiosis I(MI) in autotetraploid,autooctoploid, and autodecaploid S. spontaneum clones. All autopolyploid clones showed stable diploidized chromosome behavior;so that homologous chromosomes formed almost exclusively bivalents during MI. Two copies of homologous chromosome 8 with similar sizes in the autotetraploid clone showed preferential pairing with each other with respect to the other copies. However, sequence variation analysis showed no apparent differences among homologs of chromosome 8 and all other chromosomes. We suggest that either the stable diploidized pairing or the preferential pairing between homologous copies of chromosome 8 in the studied autopolyploid sugarcane are accounted for by unknown mechanisms other than DNA sequence similarity. Our results reveal evolutionary consequences of stable meiotic behavior in autopolyploid plants.
文摘目的结直肠癌是全球常见的恶性肿瘤之一,经自然腔道取标本手术(NOSES)作为一种微创手术,近年来在结直肠癌的治疗中得到了广泛的应用.然而,对于NOSES手术的国际研究现状尚未有系统的文献计量学分析.方法本研究利用Web of Science Core Collection数据库,对2000年至2023年间发表的关于结直肠肿瘤NOSES手术的文章进行检索,使用Bibliometrix文献计量学方法,通过对国家、机构、期刊、作者和关键词进行分析.结果本研究共纳入了316篇文献,其中2022年的最高发文量达到了44篇.中国在该领域中扮演着主要的贡献者角色,尤其是中国医学科学院&北京协和医学院,其发表的文献数量高达50篇.此外,美国的期刊《Surgical Endoscopy and Other Interventional Techniques》刊文量最多.通过聚类分析,发现研究热点仍然是NOSES技术在结直肠癌手术的应用,而中国仍然是NOSES研究的主要中心.结论本研究系统总结了2000~2023年结直肠肿瘤NOSES手术的研究成果,对全球范围内的研究趋势进行了描述,中国在引领该领域研究的发展中发挥着重要的作用.NOSES手术的技术进步和临床应用将是未来研究的重点.
基金National Natural Science Foundation of China(31471545,32171986,32100494,and 32070544)Jiangsu Agricultural Science and Technology Innovation Fund(CX(20)3139)Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang(2019R01002).
文摘Crop wild relatives are an important reservoir of natural biodiversity. However, incorporating wild geneticdiversity into breeding programs is often hampered by reproductive barriers and a lack of accurate genomicinformation. We assembled a high-quality, accurately centromere-anchored genome of Gossypium anomalum, a stress-tolerant wild cotton species. We provided a strategy to discover and transfer agronomicallyvaluable genes from wild diploid species to tetraploid cotton cultivars. With a (Gossypium hirsutum 3 G.anomalum)2 hexaploid as a bridge parent, we developed a set of 74 diploid chromosome segment substitution lines (CSSLs) of the wild cotton species G. anomalum in the G. hirsutum background. This set of CSSLsincluded 70 homozygous substitutions and four heterozygous substitutions, and it collectively containedabout 72.22% of the G. anomalum genome. Twenty-four quantitative trait loci associated with plant height,yield, and fiber qualities were detected on 15 substitution segments. Integrating the reference genome withagronomic trait evaluation of the CSSLs enabled location and cloning of two G. anomalum genes thatencode peroxiredoxin and putative callose synthase 8, respectively, conferring drought tolerance andimproving fiber strength. We have demonstrated the power of a high-quality wild-species reference genomefor identifying agronomically valuable alleles to facilitate interspecific introgression breeding in crops.
