HSP90B1-mediated plasma membrane localization of GLUT1 promotes radioresistance of glioblastomas

Ionizing radiation is a popular and effective treatment option for glioblastoma(GBM).However,resistance to radiation therapy inevitably occurs during treatment.It is urgent to investigate the mechanisms of radioresistance in GBM and to find ways to improve radiosensitivity.Here,we found that heat shock protein 90 beta family member 1(HSP90B1)was significantly upregulated in radioresistant GBM cell lines.More importantly,HSP90B1 promoted the localization of glucose transporter type 1,a key rate-limiting factor of glycolysis,on the plasma membrane,which in turn enhanced glycolytic activity and subsequently tumor growth and radioresistance of GBM cells.These findings imply that targeting HSP90B1 may effectively improve the efficacy of radiotherapy for GBM patients,a potential new approach to the treatment of glioblastoma.

Inhibition of vascular endothelial growth factor expression by Chinese medicine of Hedyotis diffusa Willd herbal compounds

The Hedyotis diffusa Willd herbal compounds(HDWHCs)are commonly used as Chinese medicine to treat cancer patients with established clinical therapeutic efficacy in China.However,the underlying mechanisms remain to be elucidated.In this study,we used freeze-dried powder of the water extracts of HDWHCs to investigate the potential mechanisms of HDWHCs in cancer treatment.HDWHCs treatment significantly inhibited vascular endothelial growth factor(VEGF)mRNA levels and VEGF transcriptional activation in cancer cells.HDWHCs also had a remarkable inhibitory effect on the expression of hypoxia-inducible factor 1alpha(HIF-1alpha).Forced expression of HIF-1αrestored VEGF transcriptional activation inhibited by HDWHCs,indicating that HDWHCs suppressed VEGF expression through decreasing HIF-1alpha expression.Moreover,HDWHCs inhibited cyclooxygenase-2(COX-2)expression,and overexpression of HIF-1alpha restored HDWHCs’inhibitory effect on COX-2 at transcriptional level.These findings may provide better understanding of HDWHCs’anti-cancer mechanism in cancer treatment.

Effect of core stability training on balance in elderly women

Objective:The study investigated the effect of core stability training on balance in elderly women.Method:Sixty female volunteers 61-70 years of age were divided into training(n=30)and control groups(n=30).Core stability training was given to patients in the training group,and walk-ing exercise was given to patients in the control group.The function of static and dynamic balance was detected before training and 30 weeks after training.Results:The time of standing on one foot with eyes closed after training in the training group was significantly longer than the control group,and the stellate balance test with stretching the lower limbs straight ahead,behind,and laterally in the training group was significantly better than the control group.The performance of two lower limbs during the training in the straight ahead di-rection,behind,and to the outer side in the training group was significantly better than the control group.Significant differences also occurred when the left leg was supported on the outer rear,and the right leg was supported on the medial front and rear.Conclusion:Core stability training is more beneficial than walking exercises in improving balance in elderly women.