[Objectives]Fifty two strains were identified and phylogenetically analyzed to solve the current phenomenon of"synonym and homonym"in morels.[Methods]Based on the ITS and LSU sequences of ribosomal DNA,the c...[Objectives]Fifty two strains were identified and phylogenetically analyzed to solve the current phenomenon of"synonym and homonym"in morels.[Methods]Based on the ITS and LSU sequences of ribosomal DNA,the collected 52 morel samples were sequenced,and BLAST alignment was performed by NCBI to construct phylogenetic trees.[Results]The collected morel strains were mainly divided into two major groups:namely black morel and yellow morel groups,of which the black morel group was most,mainly including Morchella sextelata,Morchella importuna,Morchella crassipes,and Morchella eximia.There were very few yellow morel strains,including M.crassipes and M.eximia.[Conclusions]This study lays a foundation for the classification and research of morel germplasm resources,and provides a powerful reference value for the research and production of high-quality morel strains.展开更多
This study aims at evaluating the heavy metals pollution in soil collected from Penglai Fairyland Park in Guiyang,and predicting the harm of heavy metals to human health. Based on the background value of soil in Guizh...This study aims at evaluating the heavy metals pollution in soil collected from Penglai Fairyland Park in Guiyang,and predicting the harm of heavy metals to human health. Based on the background value of soil in Guizhou and Soil Environment Quality Standard( GB15618-1995),by using the geoaccumulation index method and Hakanson potential ecological risk assessment method,we research pollution characteristics and ecological risk of heavy metals in soil from the study area. The results show that from the index of geoaccumulation,the pollution level of heavy metals in agricultural soil from Penglai Fairyland Park is in the order of Ni > Cu > Hg > Cd > As > Zn > Cr > Pb. From the potential ecological harm index evaluation,soil is at slight ecological risk level in the park and the potential ecological hazard degree is in the order of Hg > Cd > Ni > As > Cu > Cr > Pb > Zn. The soil ecological environment in research area is only mildly damaged. Thus,the soil in research area is at the level of security,and it is suitable for cultivation of crops and less harmful to human health.展开更多
Ten random primers with clear amplification profile, significant and stable main band were screened from RAPD (Random Amplified Polymorphie DNAs) primers to analyze the genetic diversity among eight Phytophthora cap...Ten random primers with clear amplification profile, significant and stable main band were screened from RAPD (Random Amplified Polymorphie DNAs) primers to analyze the genetic diversity among eight Phytophthora capsici isolates from Huaxi District, Wudang District and Kaiyang County of Guiyang City, and Zunyi County, Suiyang County and Luodian County of Zunyi City in Guizhou Province. A total of 70 DNA fingerprints were obtained, including 57 polymorphic bands, with a polymorphic percentage of 81.43%, suggesting abundant genetic diversity among experimental Phytophthora capsici isolates. According to the ampli- fied DNA fingerprint profiles, using genetic similarity coefficient 0.5 as the threshold, experimental Phytophthora capsici isolates were clustered into three genetic categories by UPGMA cluster analysis. The analysis result indicated that there was no direct correlation between the genetic similarity and cultivation areas of vari- ous Phytophthora caosici isolates.展开更多
基金Supported by Guizhou Province Science and Technology Innovation Ability Promotion Special Project[2021]1Science and Technology Project of Guizhou Province([2021]YB201,[2019]3007,[2022]YB083)。
文摘[Objectives]Fifty two strains were identified and phylogenetically analyzed to solve the current phenomenon of"synonym and homonym"in morels.[Methods]Based on the ITS and LSU sequences of ribosomal DNA,the collected 52 morel samples were sequenced,and BLAST alignment was performed by NCBI to construct phylogenetic trees.[Results]The collected morel strains were mainly divided into two major groups:namely black morel and yellow morel groups,of which the black morel group was most,mainly including Morchella sextelata,Morchella importuna,Morchella crassipes,and Morchella eximia.There were very few yellow morel strains,including M.crassipes and M.eximia.[Conclusions]This study lays a foundation for the classification and research of morel germplasm resources,and provides a powerful reference value for the research and production of high-quality morel strains.
基金Supported by Agricultural Science and Technology Achievement Transformation Fund Project of the Ministry of Science and Technology(2013F-2001007)Project of Edible Fungus Engineering Center of Guizhou Province(G20144002)+1 种基金Guiyang Science and Technology Project(201410014)Project of Baiyun District Science and Technology Bureau(201419)
文摘This study aims at evaluating the heavy metals pollution in soil collected from Penglai Fairyland Park in Guiyang,and predicting the harm of heavy metals to human health. Based on the background value of soil in Guizhou and Soil Environment Quality Standard( GB15618-1995),by using the geoaccumulation index method and Hakanson potential ecological risk assessment method,we research pollution characteristics and ecological risk of heavy metals in soil from the study area. The results show that from the index of geoaccumulation,the pollution level of heavy metals in agricultural soil from Penglai Fairyland Park is in the order of Ni > Cu > Hg > Cd > As > Zn > Cr > Pb. From the potential ecological harm index evaluation,soil is at slight ecological risk level in the park and the potential ecological hazard degree is in the order of Hg > Cd > Ni > As > Cu > Cr > Pb > Zn. The soil ecological environment in research area is only mildly damaged. Thus,the soil in research area is at the level of security,and it is suitable for cultivation of crops and less harmful to human health.
基金Supported by International Cooperation Project of Guizhou Science and Technology Department "Analysis of biological characteristics and genetic diversity of Phytophthora capsici isolates from Guizhou Province by RAPD"(QKHWGZ[2013]No.7032)
文摘Ten random primers with clear amplification profile, significant and stable main band were screened from RAPD (Random Amplified Polymorphie DNAs) primers to analyze the genetic diversity among eight Phytophthora capsici isolates from Huaxi District, Wudang District and Kaiyang County of Guiyang City, and Zunyi County, Suiyang County and Luodian County of Zunyi City in Guizhou Province. A total of 70 DNA fingerprints were obtained, including 57 polymorphic bands, with a polymorphic percentage of 81.43%, suggesting abundant genetic diversity among experimental Phytophthora capsici isolates. According to the ampli- fied DNA fingerprint profiles, using genetic similarity coefficient 0.5 as the threshold, experimental Phytophthora capsici isolates were clustered into three genetic categories by UPGMA cluster analysis. The analysis result indicated that there was no direct correlation between the genetic similarity and cultivation areas of vari- ous Phytophthora caosici isolates.