Increased evidence has shown that hydrogen sulfide(H_(2)S),a novel gasotransmitter,could enhance drought resistance in plants by inducing stomatal closure,with concurrent enhancement of photosynthetic efficiency,but l...Increased evidence has shown that hydrogen sulfide(H_(2)S),a novel gasotransmitter,could enhance drought resistance in plants by inducing stomatal closure,with concurrent enhancement of photosynthetic efficiency,but little is known about the mechanism behind this contradictory phenomenon.This study examined the regulating mechanism of H_(2)S in response to drought stress fromstomatal and non-stomatal factors in Chinese cabbage.The results showed that exogenous H_(2)S could increase the accumulation of photosynthetic pigments and alleviate the damage caused by drought stress.It also regulated the expression in transcriptional level and the activity of ribulose 1,5-bisphosphate carboxylase/oxygenase(BrRuBisCO)under drought stress.The large subunit of BrRuBisCO was found to be modified by S-sulfhydration,which might be the reason for its increased enzyme activity.The fluxes of Cl^(−),K^(+),and H^(+)in the guard cells were detected by non-invasive micro-test techniques while under drought stress.The results indicated that H_(2)S signaling induced a transmembrane Cl^(−)and H^(+)efflux and inhibited K^(+)influx,and the Cl^(−)channel was the main responders for H_(2)S-regulated stomatal movement.In conclusion,H_(2)S signal not only activated the ion channel proteins located in the guard cell membrane to induce stomatal closure,but also regulated the transcriptional expression and the activity of RuBisCO,a non-stomatal factor to enhance the photosynthetic efficiency of leaves.There is therefore a beneficial balance between the regulation of H_(2)S signaling on stomatal factors and non-stomatal factors due to drought stress,which needs to be better understood to apply it practically to increase crop yields.展开更多
Heading Chinese cabbage(Brassica rapa L.syn.B.campestris L.ssp.chinensis Makino var.pekinensis(Rupr.)J.Cao et Sh.Cao)is a cruciferous Brassica vegetable that has a triplicate genome,owing to an ancient genome duplicat...Heading Chinese cabbage(Brassica rapa L.syn.B.campestris L.ssp.chinensis Makino var.pekinensis(Rupr.)J.Cao et Sh.Cao)is a cruciferous Brassica vegetable that has a triplicate genome,owing to an ancient genome duplication event.It is unclear whether the duplicated homologs have conserved or diversi fied functions.Hydrogen sulfide(H_(2)S)is a plant gasotransmitter that plays important physiological roles in growth,development,and responses to environmental stresses.The modification of cysteines through S-sulfhydration is an important mechanism of H_(2)S,which regulates protein functions.H?S promotes flowering in Arabidopsis and heading Chinese cabbage.Here we investigated the molecular mechanisms of H_(2)S used to promote flowering in the latter.Four,five,and four BraFLC,BraSOC I,and BraFT homologs were identi fi ed in heading Chinese cabbage.Different BraFLC proteins were bound to different CArG boxes in the promoter regions of the BraSOC I and BraFT homologs,producing different binding patterns.Thus,there may be functionally diverse BraFLC homologs in heading Chinese cabbage.Exogenous H_(2)S at 100μmol L^(-1) significantly promoted flowering by compensating for insuf fi cient vernalization.BraFLC 1 and BraFLC_(3) underwent S-sulfhydration by H_(2)S,after which their abilities to bind most BraSOC I or BraFT promoter probes weakened or even disappeared.These changes in binding ability were consistent with the expression pattern of the BraFT and BraSOC I homologs in seedlings treated with H_(2)S.These results indicated that H_(2)S signaling regulates flowering time.In summary,H_(2)S signaling promoted plant flowering by weakening or eliminating the binding abilities of BraFLCs to downstream promoters through S-sulfhydration.展开更多
In the past few decades,Hydrogen sulfide(H2S)has been newly witnessed as a third gasotransmitter with similar biological functions to its two previously identified counterparts nitric oxide(NO)and carbon monoxide(CO),...In the past few decades,Hydrogen sulfide(H2S)has been newly witnessed as a third gasotransmitter with similar biological functions to its two previously identified counterparts nitric oxide(NO)and carbon monoxide(CO),after being rec-展开更多
The ubiquitous and versatile signal-transduction role of hydrogen sulfide (H2S)has been revealed in mammals and plants in recent years [1-5].It is very important to grasp the physiological concentrations of H2S in viv...The ubiquitous and versatile signal-transduction role of hydrogen sulfide (H2S)has been revealed in mammals and plants in recent years [1-5].It is very important to grasp the physiological concentrations of H2S in vivo because of its toxic characteristics at high concentrations.However,the reported data fail to reach an agreement owing to the use of different methods and technique limitations. In fact,this is a common problem for gasotransmitters.What is the exact boundary between the physiological and toxicological concentration?What is the accurate endogenous content?These problems have been plaguing us even though there are a great number of research papers that explore H2S signals.展开更多
基金funded by the National Natural Science Foundation of China(32172550 and 31972428)Shanxi Province Natural Science Foundation(20210302123431)Research Project Supported by Shanxi Scholarship Council of China(2020-014).
文摘Increased evidence has shown that hydrogen sulfide(H_(2)S),a novel gasotransmitter,could enhance drought resistance in plants by inducing stomatal closure,with concurrent enhancement of photosynthetic efficiency,but little is known about the mechanism behind this contradictory phenomenon.This study examined the regulating mechanism of H_(2)S in response to drought stress fromstomatal and non-stomatal factors in Chinese cabbage.The results showed that exogenous H_(2)S could increase the accumulation of photosynthetic pigments and alleviate the damage caused by drought stress.It also regulated the expression in transcriptional level and the activity of ribulose 1,5-bisphosphate carboxylase/oxygenase(BrRuBisCO)under drought stress.The large subunit of BrRuBisCO was found to be modified by S-sulfhydration,which might be the reason for its increased enzyme activity.The fluxes of Cl^(−),K^(+),and H^(+)in the guard cells were detected by non-invasive micro-test techniques while under drought stress.The results indicated that H_(2)S signaling induced a transmembrane Cl^(−)and H^(+)efflux and inhibited K^(+)influx,and the Cl^(−)channel was the main responders for H_(2)S-regulated stomatal movement.In conclusion,H_(2)S signal not only activated the ion channel proteins located in the guard cell membrane to induce stomatal closure,but also regulated the transcriptional expression and the activity of RuBisCO,a non-stomatal factor to enhance the photosynthetic efficiency of leaves.There is therefore a beneficial balance between the regulation of H_(2)S signaling on stomatal factors and non-stomatal factors due to drought stress,which needs to be better understood to apply it practically to increase crop yields.
基金the National Natural Science Foundation of China(31972428 and 31672140)the Shanxi Province Natural Science Foundation(201801D121191 and 201801D121197).
文摘Heading Chinese cabbage(Brassica rapa L.syn.B.campestris L.ssp.chinensis Makino var.pekinensis(Rupr.)J.Cao et Sh.Cao)is a cruciferous Brassica vegetable that has a triplicate genome,owing to an ancient genome duplication event.It is unclear whether the duplicated homologs have conserved or diversi fied functions.Hydrogen sulfide(H_(2)S)is a plant gasotransmitter that plays important physiological roles in growth,development,and responses to environmental stresses.The modification of cysteines through S-sulfhydration is an important mechanism of H_(2)S,which regulates protein functions.H?S promotes flowering in Arabidopsis and heading Chinese cabbage.Here we investigated the molecular mechanisms of H_(2)S used to promote flowering in the latter.Four,five,and four BraFLC,BraSOC I,and BraFT homologs were identi fi ed in heading Chinese cabbage.Different BraFLC proteins were bound to different CArG boxes in the promoter regions of the BraSOC I and BraFT homologs,producing different binding patterns.Thus,there may be functionally diverse BraFLC homologs in heading Chinese cabbage.Exogenous H_(2)S at 100μmol L^(-1) significantly promoted flowering by compensating for insuf fi cient vernalization.BraFLC 1 and BraFLC_(3) underwent S-sulfhydration by H_(2)S,after which their abilities to bind most BraSOC I or BraFT promoter probes weakened or even disappeared.These changes in binding ability were consistent with the expression pattern of the BraFT and BraSOC I homologs in seedlings treated with H_(2)S.These results indicated that H_(2)S signaling regulates flowering time.In summary,H_(2)S signaling promoted plant flowering by weakening or eliminating the binding abilities of BraFLCs to downstream promoters through S-sulfhydration.
基金supported by the National Natural Science Foundation of China(31372085 to Yanxi Pei,31400237 to Zhuping Jin)
文摘In the past few decades,Hydrogen sulfide(H2S)has been newly witnessed as a third gasotransmitter with similar biological functions to its two previously identified counterparts nitric oxide(NO)and carbon monoxide(CO),after being rec-
基金supported by the National Natural Science Foundation of China(31672140 to Jin ZP and 31671605 to Pei YX)
文摘The ubiquitous and versatile signal-transduction role of hydrogen sulfide (H2S)has been revealed in mammals and plants in recent years [1-5].It is very important to grasp the physiological concentrations of H2S in vivo because of its toxic characteristics at high concentrations.However,the reported data fail to reach an agreement owing to the use of different methods and technique limitations. In fact,this is a common problem for gasotransmitters.What is the exact boundary between the physiological and toxicological concentration?What is the accurate endogenous content?These problems have been plaguing us even though there are a great number of research papers that explore H2S signals.
