Diagenetic evolution and effects on reservoir development of the Dengying and Longwangmiao formations,Central Sichuan Basin,Southwestern China

The deeply buried Lower Cambrian Longwangmiao Formation and Upper Ediacaran Dengying Formation from the Sichuan Basin,China,have a total natural gas reserve up to 3×10^(12)m^(3).The complex diagenetic evolution and their impacts on the present-day reservoir quality have not been systematically elucidated,hampering the current exploration.Crucially,the integration and comparation diagenetic study on these two formations,which may be able to shed new lights on reservoir formation mechanism,are yet to be systemically evaluated.By compiling geochemistry data,including carbonate U-Pb ages and petrophysics data,coupled with new petrology,trace elements,and strontium isotope data,of various types of diagenetic carbonates,this study aims to decipher the potential links between diagenesis and reservoir development of both formations.Intriguingly,similar diagenetic sequence,which contains five distinctive dolomite phases,is established in both formations.The matrix dolomite(D1)and early dolomite cement(D2)were likely formed by reflux dolomitization,as inferred by their nearly syn-depositional U-Pb ages and elevatedδ^(18)O caused by seawater evaporation.The subsequent moderate burial dolomite cement(D3)was most plausibly the product of burial compaction as indicated by its lighterδ^(18)O and slightly younger U-Pb ages compared with D1 and D2.Whereas deep burial dolomite cements(D4 and D5)yield markedly depletedδ^(18)O,elevated ^(87)Sr/^(86)Sr,along with much younger U-Pb ages and higher precipitation temperatures,suggesting that they were likely linked to hydrothermal fluids.Despite the wide occurrence of meteoric and organic acids leaching and thermochemical sulfate reduction,they may have only played a subsidiary role on these reservoirs development.Instead,superior reservoir quality is tightly linked to tectonics as inferred by higher reservoir quality closely related to the well-developed fractures and faults filled with abundant hydrothermal minerals.Notably,good reservoirs in both formations are mainly attributed to high permeability caused by tectonics.Hence,this new contribution emphasizes the crucial role of tectonics on spatially explicit reservoir prediction of deep to ultra-deep(up to>8000 m)carbonates in the Sichuan Basin,as well as other sedimentary basin analogues in China.

飞秒激光小切口角膜基质透镜取出术中旋转补偿对散光矫正疗效的Meta分析

目的:比较飞秒激光小切口角膜基质透镜取出术(SMILE)术中旋转补偿对散光矫正的疗效。方法:检索PubMed、Web of Science、EMBASE、Cochrane以及CNKI、VIP、CBM和Wan Fang Data数据库,纳入2010-01/2022-08试验组为SMILE术中进行旋转补偿;对照组为SMILE术中不进行旋转补偿的临床对照研究。由两名研究员独立进行文献筛选、质量评价和数据提取后,使用Stata16.0软件对术后裸眼远视力(UDVA)、残余散光、矢量分析法用于衡量散光矫正效果的指标[包括误差角度的绝对值(|AE|):手术矫正散光的轴向与预期的误差绝对值;误差大小(ME):手术矫正散光与预期的算数差值]和术后总高阶像差、球差、彗差进行Meta分析。结果:最终纳入7篇文献,共846眼(试验组442眼,对照组404眼),Meta分析结果显示,两组患者在残余散光≥1.00D的眼所占百分比(OR=0.17,95%CI:0.06~0.49,P<0.01)、|AE|(WMD=-1.56,95%CI:-2.68~-0.45,P<0.01)、彗差(WMD=0.06,95%CI:-0.08~-0.04,P<0.01)和总高阶像差(WMD=-0.04,95%CI:-0.06~-0.02,P<0.01)均有差异,但在术后UDVA(WMD=0.00,95%CI:-0.02~0.01,P=0.54)、残余散光度数(WMD=0.08,95%CI:-0.02~0.18,P=0.10)、ME(WMD=-0.01,95%CI:-0.14~0.12,P=0.85)及球差(WMD=0.03,95%CI:-0.07~0.13,P=0.52)均无差异。结论:SMILE术中进行旋转补偿能有效减少散光矫正时因眼球旋转所致的角度误差、降低术后残余散光,在精确矫正散光的临床疗效上更具优势。

OCTA对湿性年龄相关性黄斑变性脉络膜新生血管诊断价值的Meta分析

目的:采用Meta分析方法评价相干光断层扫描血管成像(OCTA)对湿性年龄相关性黄斑变性(wARMD)患者脉络膜新生血管(CNV)的诊断价值。方法:计算机检索PubMed、Embase、Web of science、Cochrane library、CNKI、Wanfang、CBM和VIP数据库建库至2020-10-27关于OCTA诊断wARMD患者CNV的相关研究。由两位研究员独立筛选文献、提取资料并采用QUADAS-2工具评价纳入研究的偏倚风险,使用Meta-Disc 1.4及Stata 16.0软件进行Meta分析。结果:纳入文献11篇,患者995眼。OCTA诊断wARMD患者CNV的Meta分析结果显示,合并灵敏度、特异度、阳性似然比、阴性似然比、诊断比值比、sROC曲线下面积和阳性验后概率依次为0.88[95%CI(0.83,0.92)]、0.95[95%CI(0.85,0.99)]、18.45[95%CI(5.36,63.52)]、0.12[95%CI(0.08,0.18)]、152.73[95%CI(36.39,641.05)]、0.95[95%CI(0.92,0.96)]和0.96。结论:相干光断层扫描血管成像用于诊断wARMD患者CNV具有较高价值,尤其适用于wARMD的早期诊断。

Listeria monocytogenes bacteremia in a centenarian and pathogen traceability:A case report

BACKGROUND Early diagnosis and appropriate antibiotic treatment are important to survival of Listeria monocytogenes(L.monocytogenes)bacteremia.Penicillin tends to be the most commonly used antibiotic.However,there are limited data on antibiotic use in elderly patients with serious complications.We describe the clinical presentation,antibiotic therapy,and traceability of L.monocytogenes in a centenarian with a history of eating frozen food.CASE SUMMARY A 102-year-old man suffered from high fever with chill after hematochezia.Tentative diagnoses were lower gastrointestinal hemorrhage and localized peritonitis.Meropenem and ornidazole were the empirical therapy.The patient did not respond and developed multiple system dysfunction even after teicoplanin was added to the therapy.L.monocytogenes was identified from blood cultures on day 5 of admission.The patient had a history of consuming frozen dumplings.Meropenem/ornidazole/teicoplanin were replaced with meropenem/linezolid.The patient gradually became afebrile.He received meropenem/linezolid for 10 d,and piperacillin/tazobactam was applied as step-down treatment for 2 wk with good clinical results.There was no sign of relapse during follow-up after discharge.L.monocytogenes isolates from the patient and frozen dumplings belonged to different serotypes and sequence types(STs):1/2b and ST5 from the patient and 1/2c and ST9 from the dumplings.CONCLUSION More awareness of listeriosis should be raised.Linezolid might be an option for listeriosis in elderly people with serious complications.

针刺对轻度至中度阻塞性睡眠呼吸暂停综合征患者的疗效:一项随机对照临床试验方案

Introduction:Obstructive sleep apnea syndrome(OSAS) is considered a major public health issue due to its high prevalence and severe consequences.Several published studies have revealed that electroacupuncture may relieve OSAS symptoms.However,the mechanism of electro-acupuncture in the treatment of patients with OSAS remains unclear.Methods and analysis:We evaluated the efficacy and mechanism of electro-acupuncture in the treatment of patients with OSAS.This was a two-group parallel randomized controlled trial.Sixty patients with OSAS will be randomly divided into an electro-acupuncture or shallow acupuncture group in a proportion of 1:1,and the patients will be administered 36 sessions of electro/shallow acupuncture treatment(three times a week for 12 weeks,20 min for each session).The Epworth Sleepiness Scale(ESS) will be used as the primary outcome.Secondary outcomes will include polysomnography(PSG),sleep quality,emotional state,and cognitive function.In addition,patients will receive neuroimaging scanning before and after the entire acupuncture treatment to evaluate neuroplasticity changes,including gray matter density,white matter integrity,and functional connections.Expected outcomes:We believe that the use of electro-acupuncture will be more effective in patients with mild to moderate OSAS and that the cognitive and emotional status of patients will improve after acupuncture treatment.In addition,we aim to clarify which brain areas can be altered by acupuncture in patients with OSAS.Discussion:This randomized clinical trial aims to explore the curative effect and central mechanism of acupuncture treatment for OSAS.We will explore gray matter density,white matter integrity,and functional connections of the brain using fMRI technology.The results of this study will be significant to the guidelines for clinical OSAS treatment.

Effect of 43 cases of temporomandibular joint dysfunction treated with thumb-tack needle based on the meridian cutaneous region theory

Objective： To observe the clinical efficacy of thumb-tack needle on temporomandibular joint dysfunction（TMD）.Methods： A total of 43 outpatients of TMD were treated with thumb-tack needle. After the skin routine sterilization, the intradermal needles, 0.2 mm x 1.5 mm（the SEIRIN-thumb-tack needle） were embedded subcutaneously at Zúlinqì（足临泣GB 41）, Zhōngzhǔ（中渚 TE 3） and Xiàguān（下关 ST 7） on the affected side. The acupoints were pressed and kneaded gently to induce mild soreness and distention.When pressing and kneading GB 41 and TE 3, the patients were required to open and close the mouth in small amplitude to achieve the effects of kinetic acupuncture. The treatment was given twice a week.The needles were retained for 24 h. A total of 6 treatments were required.Results： A total of 22 cases were cured, accounting for 51.16%, 15 cases effective remarkably, accounting for 34.88% and 6 cases failed, accounting for 13.95%. The total effective rate was 86.05%.Conclusion： The thumb-tack needle is significantly effective on TMD.

Metal-Free Catalyzed Cyclization of N-Methoxybenzamides to Construct Quaternary Carbon-Containing Isoindolinones

Main observation and conclusion Through the intramolecular cyclization of N-methoxybenzamides,a simple and efficient method for constructing valuable isoindolinones under metal-free conditions was developed.The reaction was featured by employing low-cost catalyst,simple operation,100%atomic economy and excellent regioselectivity.Moreover,a detailed computational study on the reaction system has been performed to clarify the mechanism.

Evaluation on Hot/Cold Drug Property of Acaí(Euterpe oleracea Mart.) by Cytological Study Methods

Objective: To determine the hot/cold of South American A?aí(Euterpe oleracea Mart.). Materials and Methods: 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay was performed to compare the influence of A?aí and phellodendron bark(Cortex Phellodendri Chinensis) on the growth and proliferation of HepG2 and Hep3 B cell strains. The morphological changes of the two cell strains treated with A?aí and C. Phellodendri were observed using an inverted phase contrast microscope. Trypan blue dye exclusion assay was used to compare and analyze the toxicity effects of A?aí. The content of nicotinamide adenine dinucleotide(NADH) and the ratio of NADH/NAD+ of the treated cells were detected using a spectrophotometer to determine the influence of A?aí to the energy metabolism of the two cell strains. Results: Within the range of measured concentrations, both A?aí and C. Phellodendri displayed separately the stronger inhibitory effects on cell growth, proliferation, and energy metabolism of the two cell strains. By MTT assay, A?aí showed significant(P < 0.05) or highly significant(P < 0.01) inhibitory effects on cell proliferation within the concentration range of 0.25–40 μg/mL, which was similar to the effects of C. Phellodendri. From the results of microscopic examination, the morphological of lower cell density, attenuated granularity, and more outstretched cells in irregular polygonal shape could be observed in the cells treated with A?aí, which was also similar to that of C. Phellodendri. In trypan blue staining assay, all concentrations of A?aí showed negligible toxicity effects, exactly as that of C. Phellodendri. A?aí showed a highly significant effect of decreasing the content of intracellular NADH as well as the ratio of NADH/NAD+(P < 0.05 and P < 0.01, respectively). Conclusion: The effect of A?aí on HepG2 and Hep3 B strains just as C. Phellodendri can reduce energy metabolism and inhibit the growth and proliferation. All performance characteristics of two cell strains treated with A?aí belong to that characteristic of cold property drug. From the study results, we can deduce that the drug property of A?aí is cold.