Metal halide perovskites have attracted much attention in biomedicine because of their excellent fluorescence energy conversion properties;however,poor water-stability and cytotoxicity limit its applications as a biom...Metal halide perovskites have attracted much attention in biomedicine because of their excellent fluorescence energy conversion properties;however,poor water-stability and cytotoxicity limit its applications as a biomedical tracer,especially in cellular imaging.Herein,water-ultrastable perovskites C sPbBr_(3):Cs_(4)PbBr_(6)nanocrystals(NCs)encapsulated in chitosan are fabricated successfully using a water-triggered method.The as-synthesized CsPbBr_(3):Cs_(4)PbBr_(6)@CS(chitosan,CS)nanoparticles in water display enhanced fluorescence emission for 35 days.Further,the viability of glioma cells(U87 cells)incubated with different concentrations of CsPbBr_(3):Cs_(4)PbBr_(6)@CS nanoparticles(0-20μg·ml^(-1))for24 h is found to be higher than 90%.In artificial body fluid,analyses using laser confocal microscopy,the standard Cell Counting Kit-8(CCK-8)method,and flow cytometry demonstrated the good water ultrastability and high biocompatibility performance of CsPbBr_(3):Cs_(4)PbBr_(6)@CS nanoparticles in cellular imaging.Overall,the water-ultrastable halide perovskites support promising perspectives in biological cell tracing and intelligent medical technology.展开更多
基金financially supported by the National Natural Science Foundation of China(Nos.U2130128,11772207)the Natural Science Foundation of Hebei Province(Nos.A2019210204,H2022205047)+4 种基金the Central Government Guiding Local Science and Technology Development Project(No.216Z4302G)the Natural Science Foundation of Hebei Education Department(No.ZD2020192)the Youth Top-notch Talents Supporting Plan of Hebei ProvinceNational Key Research and Development Program of China(No.2016YFC0904503)Hebei Administration for Market Supervision Science and Technology Project List(2023ZC03)。
文摘Metal halide perovskites have attracted much attention in biomedicine because of their excellent fluorescence energy conversion properties;however,poor water-stability and cytotoxicity limit its applications as a biomedical tracer,especially in cellular imaging.Herein,water-ultrastable perovskites C sPbBr_(3):Cs_(4)PbBr_(6)nanocrystals(NCs)encapsulated in chitosan are fabricated successfully using a water-triggered method.The as-synthesized CsPbBr_(3):Cs_(4)PbBr_(6)@CS(chitosan,CS)nanoparticles in water display enhanced fluorescence emission for 35 days.Further,the viability of glioma cells(U87 cells)incubated with different concentrations of CsPbBr_(3):Cs_(4)PbBr_(6)@CS nanoparticles(0-20μg·ml^(-1))for24 h is found to be higher than 90%.In artificial body fluid,analyses using laser confocal microscopy,the standard Cell Counting Kit-8(CCK-8)method,and flow cytometry demonstrated the good water ultrastability and high biocompatibility performance of CsPbBr_(3):Cs_(4)PbBr_(6)@CS nanoparticles in cellular imaging.Overall,the water-ultrastable halide perovskites support promising perspectives in biological cell tracing and intelligent medical technology.
