Flood is one of a kind of disasters which harms human and animal life around the globe. Pakistan has been observing massive floods for many years because of daily and seasonal variation in the temperature levels. Whea...Flood is one of a kind of disasters which harms human and animal life around the globe. Pakistan has been observing massive floods for many years because of daily and seasonal variation in the temperature levels. Wheat, rice, sugarcane and cotton are major crops cultivated in Punjab region of Pakistan in which rice and sugarcane are mostly effected by floods. In this research paper, damage assessment of cultivated land in district Hafizabad along Chenab River has been calculated. Supervised Classification and Soil Adjusted Vegetation Index (SAVI) methods are applied. Pre-flood 2014, post-flood 2014, and pre-flood 2015 Landsat 8 images have been used to calculate the extent of damages to cultivated lands. Water, sand, silt, bare soil and vegetation are classified to identify damage. Results show that vegetation cover has plummeted to 50% after the arrival of flood 2014 in the Chenab. Similarly, 6.7047% of sand and 15.7339% of bare soil deposits have surfaced which have not yet been removed from fertile lands in 2015. 18.4376% standing crop damage has been analyzed under this study. 14.0245% silt deposits have been calculated as post-flood effects. 46.4260% land has been cultivated in 2015 which is 15.5024% lower than 2014 cultivated land. Furthermore, field verification survey has given promising results and has a great correlation with satellite based recovery results.展开更多
World energy consumption increased by 56 percent, from 524 quadrillion Btu in 2010 to 820 quadrillion Btu in 2040. The increased demand in energy consumption is fulfilled by different renewable and non-renewable sourc...World energy consumption increased by 56 percent, from 524 quadrillion Btu in 2010 to 820 quadrillion Btu in 2040. The increased demand in energy consumption is fulfilled by different renewable and non-renewable sources such as petroleum, natural gas, electricity, nuclear etc. Natural gas is one of the most important sources of energy. SNGPL has been managing a 94,263 km long gas pipelines network covering approximately the northern part of Pakistan. In this paper we have presented the use of condition based maintenance (CBM) management techniques with a?geographical information system (GIS) for asset management of a gas distribution network of?SNGPL. The continuous monitoring and updating of asset data reveal where the assets are located and which needs maintenance or which lies in critical condition. The system helps to save time and reduce visits to the sites and labour reduction.展开更多
Pretreatment of lignocellulosic biomass is crucial for the release of biofermentable sugars for biofuels production,which could greatly alleviate the burgeoning environment and energy crisis caused by the massive usag...Pretreatment of lignocellulosic biomass is crucial for the release of biofermentable sugars for biofuels production,which could greatly alleviate the burgeoning environment and energy crisis caused by the massive usage of traditional fossil fuels.Pyrolysis is a cost-saving pretreatment process that can readily decompose biomass into levoglucosan,a promising anhydrosugar;however,many undesired toxic compounds inhibitory to downstream microbial fermentation are also generated during the pyrolysis,immensely impeding the bioconversion of levoglucosan-containing pyrolysate.Here,we took the first insight into the proteomic responses of a levoglucosan-utilizing and ethanol-producing Escherichia coli to three representative biomass-derived inhibitors,identifying large amounts of differentially expressed proteins(DEPs)that could guide the downstream metabolic engineering for the development of inhibitor-resistant strains.Fifteen up-and eight down-regulated DEPs were further identified as the biomarker stress-responsive proteins candidate for cellular tolerance to multiple inhibitors.Among these biomarker proteins,YcfR exhibiting the highest expression fold-change level was chosen as the target of overexpression to validate proteomics results and develop robust strains with enhanced inhibitor tolerance and fermentation performance.Finally,based on four plasmid-borne genes encoding the levoglucosan kinase,pyruvate decarboxylase,alcohol dehydrogenase,and protein YcfR,a new recombinant strain E.coli LGE-ycfR was successfully created,showing much higher acetic acid-,furfural-,and phenol-tolerance levels compared to the control without overexpression of ycfR.The specific growth rate,final cell density,ethanol concentration,ethanol productivity,and levoglucosan consumption rate of the recombinant were also remarkably improved.From the proteomics-guided metabolic engineering and phenotypic observations,we for the first time corroborated that YcfR is a stress-induced protein responsive to multiple biomass-derived inhibitors,and also developed an inhibitors-resistant strain that could produce bioethanol from levoglucosan in the presence of inhibitors of relatively high concentration.The newly developed E.coli LGE-ycfR strain that could eliminate the commonly-used costly detoxicification processes,is of great potential for the in situ cost-effective bioethanol production from the biomass-derived pyrolytic substrates.展开更多
文摘Flood is one of a kind of disasters which harms human and animal life around the globe. Pakistan has been observing massive floods for many years because of daily and seasonal variation in the temperature levels. Wheat, rice, sugarcane and cotton are major crops cultivated in Punjab region of Pakistan in which rice and sugarcane are mostly effected by floods. In this research paper, damage assessment of cultivated land in district Hafizabad along Chenab River has been calculated. Supervised Classification and Soil Adjusted Vegetation Index (SAVI) methods are applied. Pre-flood 2014, post-flood 2014, and pre-flood 2015 Landsat 8 images have been used to calculate the extent of damages to cultivated lands. Water, sand, silt, bare soil and vegetation are classified to identify damage. Results show that vegetation cover has plummeted to 50% after the arrival of flood 2014 in the Chenab. Similarly, 6.7047% of sand and 15.7339% of bare soil deposits have surfaced which have not yet been removed from fertile lands in 2015. 18.4376% standing crop damage has been analyzed under this study. 14.0245% silt deposits have been calculated as post-flood effects. 46.4260% land has been cultivated in 2015 which is 15.5024% lower than 2014 cultivated land. Furthermore, field verification survey has given promising results and has a great correlation with satellite based recovery results.
文摘World energy consumption increased by 56 percent, from 524 quadrillion Btu in 2010 to 820 quadrillion Btu in 2040. The increased demand in energy consumption is fulfilled by different renewable and non-renewable sources such as petroleum, natural gas, electricity, nuclear etc. Natural gas is one of the most important sources of energy. SNGPL has been managing a 94,263 km long gas pipelines network covering approximately the northern part of Pakistan. In this paper we have presented the use of condition based maintenance (CBM) management techniques with a?geographical information system (GIS) for asset management of a gas distribution network of?SNGPL. The continuous monitoring and updating of asset data reveal where the assets are located and which needs maintenance or which lies in critical condition. The system helps to save time and reduce visits to the sites and labour reduction.
基金The authors gratefully acknowledge the financial support of the National Natural Science Foundation of China(Grants NO.21978287)the Fundamental Research Funds for the Central Universities(Y954035XX2).
文摘Pretreatment of lignocellulosic biomass is crucial for the release of biofermentable sugars for biofuels production,which could greatly alleviate the burgeoning environment and energy crisis caused by the massive usage of traditional fossil fuels.Pyrolysis is a cost-saving pretreatment process that can readily decompose biomass into levoglucosan,a promising anhydrosugar;however,many undesired toxic compounds inhibitory to downstream microbial fermentation are also generated during the pyrolysis,immensely impeding the bioconversion of levoglucosan-containing pyrolysate.Here,we took the first insight into the proteomic responses of a levoglucosan-utilizing and ethanol-producing Escherichia coli to three representative biomass-derived inhibitors,identifying large amounts of differentially expressed proteins(DEPs)that could guide the downstream metabolic engineering for the development of inhibitor-resistant strains.Fifteen up-and eight down-regulated DEPs were further identified as the biomarker stress-responsive proteins candidate for cellular tolerance to multiple inhibitors.Among these biomarker proteins,YcfR exhibiting the highest expression fold-change level was chosen as the target of overexpression to validate proteomics results and develop robust strains with enhanced inhibitor tolerance and fermentation performance.Finally,based on four plasmid-borne genes encoding the levoglucosan kinase,pyruvate decarboxylase,alcohol dehydrogenase,and protein YcfR,a new recombinant strain E.coli LGE-ycfR was successfully created,showing much higher acetic acid-,furfural-,and phenol-tolerance levels compared to the control without overexpression of ycfR.The specific growth rate,final cell density,ethanol concentration,ethanol productivity,and levoglucosan consumption rate of the recombinant were also remarkably improved.From the proteomics-guided metabolic engineering and phenotypic observations,we for the first time corroborated that YcfR is a stress-induced protein responsive to multiple biomass-derived inhibitors,and also developed an inhibitors-resistant strain that could produce bioethanol from levoglucosan in the presence of inhibitors of relatively high concentration.The newly developed E.coli LGE-ycfR strain that could eliminate the commonly-used costly detoxicification processes,is of great potential for the in situ cost-effective bioethanol production from the biomass-derived pyrolytic substrates.
