Profiles of immune status and related pathways in sepsis: evidencebased on GEO and bioinformatics

Sepsis,characterized as life-threatening sequential organ failure,is caused by a dysregulated host immune response to a pathogen.Conventional practice for sepsis is to control the inflammation source and administer highgrade antibiotics.However,the mortality rate of sepsis varies from 25–30%and can reach 50%if a septic shock occurs.In our current study,we used bioinformatics technology to detect immune status profiles in sepsis at the genomic level.We downloaded and analyzed gene expression profiles of GSE28750 from the Gene Expression Omnibus(GEO)database to determine differential gene expression and immune status between sepsis and normal samples.Next,we used the CIBERSORT method to quantify the proportions of immune cells in the sepsis samples.Then we explored the differentially expressed genes(DEGs)related to sepsis.Furthermore,gene ontology(GO)function and the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were used to present potential signaling pathways in sepsis.We found that in the sepsis samples,the CD8+T cell fraction was consistently lower,based on the CIBERSORT method,whereas the neutrophil fraction was significantly higher in the sepsis samples.The GO function and KEGG pathway enrichment analysis identified 1573 DEGs that were significantly associated with neutrophil activation,neutrophil degranulation,neutrophil activation involved in the immune response,neutrophil-mediated immunity,and T cell activation in the biological processes group.In our study,we provided a first glance of associations between immune status and sepsis.Furthermore,our data regarding the reciprocal interaction between immune cells(neutrophils and CD8+T cells)could improve our understanding of immune status profiles in sepsis.However,additional investigations should be performed to verify their clinical value.

Experimental Investigation on Vibration Reduction Performance of Fiber Metal Laminate Beams with MRE Core

The vibration reduction characteristics of composite beams filled with magnetorheological elastomer core are studied experimentally.The fiber metal laminates with magnetorheological elastomers core is self-designed and prepared.Internal magnetic field is applied to the beam to explore its action of damping vibration performance under the magnetic field for the first time.The composite elements test system with controllable magnetic field intensity is designed and the function of each part is introduced.Then,a set of reasonable and standard vibration test flow of this type of composite beam under different magnetic field intensity is clarified,and the practical test is conducted.It has been found that the composite beam has excellent damping performance with the first 4 damping ratios being greater than 10%.Moreover,after the magnetic field is applied,its damping results can be further improved to meet the active control purpose.