Enhancing photosynthetic efficiency is a major goal for improving crop yields under agricultural field conditions and is associated with chloroplast biosynthesis and development.In this study,we demonstrate that Golde...Enhancing photosynthetic efficiency is a major goal for improving crop yields under agricultural field conditions and is associated with chloroplast biosynthesis and development.In this study,we demonstrate that Golden2-like 1a(BnGLK1a)plays an important role in regulating chloroplast development and photosynthetic efficiency.Overexpressing BnGLK1a resulted in significant increases in chlorophyll content,the number of thylakoid membrane layers and photosynthetic efficiency in Brassica napus,while knocking down BnGLK1a transcript levels through RNA interference(RNAi)had the opposite effects.A yeast two-hybrid screen revealed that BnGLK1a interacts with the abscisic acid receptor PYRABACTIN RESISTANCE 1-LIKE 1-2(BnPYL1-2)and CONSTITUTIVE PHOTOMORPHOGENIC 9 SIGNALOSOME 5A subunit(BnCSN5A),which play essential roles in regulating chloroplast development and photosynthesis.Consistent with this,BnGLK1a-RNAi lines of B.napus display hypersensitivity to the abscisic acid(ABA)response.Importantly,overexpression of BnGLK1a resulted in a 10%increase in thousand-seed weight,whereas seeds from BnGLK1a-RNAi lines were 16%lighter than wild type.We propose that BnGLK1a could be a potential target in breeding for improving rapeseed productivity.Our results not only provide insights into the mechanisms of BnGLK1a function,but also offer a potential approach for improving the productivity of Brassica species.展开更多
A 10‐MJ‐class superconducting magnetic energy storage(SMES)magnet is designed and optimized in this study using quasi‐isotropic strands and stacked‐tape conductors.In order to ensure the stable operation of SMES s...A 10‐MJ‐class superconducting magnetic energy storage(SMES)magnet is designed and optimized in this study using quasi‐isotropic strands and stacked‐tape conductors.In order to ensure the stable operation of SMES systems,it is necessary to evaluate the mechanical properties risk caused by the Lorentz force.Therefore,in this study,the magnetic stress caused by the Lorentz force is analyzed using the finite element method.The results show that the tapes near the inner diameter of the magnet are subjected to a higher stress and require considerable support.Although the maximum stress is increased by two times due to the presence of the screening current,it is within the safety range.The screening current does not vanish after the discharge process.After discharge,the coil is still subjected to a stress on the other of a few MPa.展开更多
基金This work was funded by the National Natural Science Foundation of China(32172597 and 31830067)the Chongqing Talents of Exceptional Young Talents Project,China(CQYC202005097,cstc2021ycjh-bgzxm0204,and cstc2021jcyj-bshX0002)+2 种基金the China Agriculture Research System of MOF and MARA(CARS-12)the 111 Project,China(B12006)the Germplasm Creation Special Program of Southwest University,China。
文摘Enhancing photosynthetic efficiency is a major goal for improving crop yields under agricultural field conditions and is associated with chloroplast biosynthesis and development.In this study,we demonstrate that Golden2-like 1a(BnGLK1a)plays an important role in regulating chloroplast development and photosynthetic efficiency.Overexpressing BnGLK1a resulted in significant increases in chlorophyll content,the number of thylakoid membrane layers and photosynthetic efficiency in Brassica napus,while knocking down BnGLK1a transcript levels through RNA interference(RNAi)had the opposite effects.A yeast two-hybrid screen revealed that BnGLK1a interacts with the abscisic acid receptor PYRABACTIN RESISTANCE 1-LIKE 1-2(BnPYL1-2)and CONSTITUTIVE PHOTOMORPHOGENIC 9 SIGNALOSOME 5A subunit(BnCSN5A),which play essential roles in regulating chloroplast development and photosynthesis.Consistent with this,BnGLK1a-RNAi lines of B.napus display hypersensitivity to the abscisic acid(ABA)response.Importantly,overexpression of BnGLK1a resulted in a 10%increase in thousand-seed weight,whereas seeds from BnGLK1a-RNAi lines were 16%lighter than wild type.We propose that BnGLK1a could be a potential target in breeding for improving rapeseed productivity.Our results not only provide insights into the mechanisms of BnGLK1a function,but also offer a potential approach for improving the productivity of Brassica species.
基金supported in part by the National Natural Science Foundation of China under Grant No.51977078.
文摘A 10‐MJ‐class superconducting magnetic energy storage(SMES)magnet is designed and optimized in this study using quasi‐isotropic strands and stacked‐tape conductors.In order to ensure the stable operation of SMES systems,it is necessary to evaluate the mechanical properties risk caused by the Lorentz force.Therefore,in this study,the magnetic stress caused by the Lorentz force is analyzed using the finite element method.The results show that the tapes near the inner diameter of the magnet are subjected to a higher stress and require considerable support.Although the maximum stress is increased by two times due to the presence of the screening current,it is within the safety range.The screening current does not vanish after the discharge process.After discharge,the coil is still subjected to a stress on the other of a few MPa.
