The global Coronavirus disease 2019(COVID-19)pandemic caused by SARS-CoV-2 has affected more than eight million people.There is an urgent need to investigate how the adaptive immunity is established in COVID-19 patien...The global Coronavirus disease 2019(COVID-19)pandemic caused by SARS-CoV-2 has affected more than eight million people.There is an urgent need to investigate how the adaptive immunity is established in COVID-19 patients.In this study,we proled adaptive immune cells of PBMCs from recovered COVID-19 patients with varying disease severity using single-cell RNA and TCR/BCR V(D)J sequencing.The sequencing data revealed SARS-CoV-2-specic shufing of adaptive immune repertories and COVID-19-induced remodeling of peripheral lymphocytes.Characterization of variations in the peripheral T and B cells from the COVID-19 patients revealed a positive correlation of humoral immune response and T-cell immune memory with disease severity.Sequencing and functional data revealed SARS-CoV-2-specic T-cell immune memory in the convalescent COVID-19 patients.Furthermore,we also identied novel antigens that are responsive in the convalescent patients.Altogether,our study reveals adaptive immune repertories underlying pathogenesis and recovery in severe versus mild COVID-19 patients,providing valuable information for potential vaccine and therapeutic development against SARS-CoV-2 infection.展开更多
基金funded by the National Natural Science Foundation of China grant no.31825008 and 31422014 to Z.H.and grant no.61872117 to F.Z.
文摘The global Coronavirus disease 2019(COVID-19)pandemic caused by SARS-CoV-2 has affected more than eight million people.There is an urgent need to investigate how the adaptive immunity is established in COVID-19 patients.In this study,we proled adaptive immune cells of PBMCs from recovered COVID-19 patients with varying disease severity using single-cell RNA and TCR/BCR V(D)J sequencing.The sequencing data revealed SARS-CoV-2-specic shufing of adaptive immune repertories and COVID-19-induced remodeling of peripheral lymphocytes.Characterization of variations in the peripheral T and B cells from the COVID-19 patients revealed a positive correlation of humoral immune response and T-cell immune memory with disease severity.Sequencing and functional data revealed SARS-CoV-2-specic T-cell immune memory in the convalescent COVID-19 patients.Furthermore,we also identied novel antigens that are responsive in the convalescent patients.Altogether,our study reveals adaptive immune repertories underlying pathogenesis and recovery in severe versus mild COVID-19 patients,providing valuable information for potential vaccine and therapeutic development against SARS-CoV-2 infection.
