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脂肪移植后脂肪组织的血管和脂肪生成
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作者 ziqing dong Zhangsong Peng +8 位作者 Qiang Chang Weiqing Zhan Zhaowei Zeng Shengchang Zhang Feng Lu 叶美辰 高景恒 张晨 袁继龙 《中国美容整形外科杂志》 CAS 2015年第10期I0016-I0016,共1页
背景脂肪移植的主要缺点是临床上不可预测,所以常会导致外科医师和患者对结果不满意。目前,移植组织血管化和重建的机制尚不明确。方法用两种不同的方法(打碎和完整保留)处理大鼠脂肪组织,以明确移植血管生成的方式。将正常大鼠和... 背景脂肪移植的主要缺点是临床上不可预测,所以常会导致外科医师和患者对结果不满意。目前,移植组织血管化和重建的机制尚不明确。方法用两种不同的方法(打碎和完整保留)处理大鼠脂肪组织,以明确移植血管生成的方式。将正常大鼠和表达绿色荧光蛋白转基因大鼠的组织进行交叉移植,观察脂肪再生过程中细胞的起源。结果几乎所有新血管的CD31内皮细胞都来源于受体组织。移植组织中新的血管主要通过受体组织中的血管长入形成的,而不是移植组织的内皮细胞重组或受体组织和移植组织血管的重建形成的。血管生成主要依赖于移植的环境。移植后8周,移植的脂肪组织容量仅下降10%,在脂肪形成过程中,主要再生细胞(CD34+)大部分来自受体组织(P〈0.05)。组织的保留在脂肪打碎组中上升73%,在脂肪完整组中上升47.5%。结论脂肪移植的血管生成主要是通过传统的“血管分支”方式,受体组织起到重要的作用。移植组织的保留方式主要涉及到来源于受体组织的CD34’脂肪前体细胞。 展开更多
关键词 组织血管化 脂肪生成 脂肪组织 移植后 受体组织 转基因大鼠 内皮细胞 移植组织
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Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration
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作者 Ye Li Xin Bi +4 位作者 Mengfan Wu Xinyao Chen Weiqing Zhan ziqing dong Feng Lu 《Burns & Trauma》 SCIE 2023年第1期217-228,共12页
Background:Large-area soft tissue defects are challenging to reconstruct.Clinical treatment methods are hampered by problems associated with injury to the donor site and the requirement for multiple surgical procedure... Background:Large-area soft tissue defects are challenging to reconstruct.Clinical treatment methods are hampered by problems associated with injury to the donor site and the requirement for multiple surgical procedures.Although the advent of decellularized adipose tissue(DAT)offers a new solution to these problems,optimal tissue regeneration efficiency cannot be achieved because the stiffness of DAT cannot be altered in vivo by adjusting its concentration.This study aimed to improve the efficiency of adipose regeneration by physically altering the stiffness of DAT to better repair large-volume soft tissue defects.Methods:In this study,we formed three different cell-free hydrogel systems by physically crosslinking DAT with different concentrations of methyl cellulose(MC;0.05,0.075 and 0.10 g/ml).The stiffness of the cell-free hydrogel system could be regulated by altering the concentration of MC,and all three cell-free hydrogel systems were injectable and moldable.Subsequently,the cell-free hydrogel systems were grafted on the backs of nude mice.Histological,immunofluorescence and gene expression analyses of adipogenesis of the grafts were performed on days 3,7,10,14,21 and 30.Results:The migration of adipose-derived stem cells(ASCs)and vascularization were higher in the 0.10 g/ml group than in the 0.05 and 0.075 g/ml groups on days 7,14 and 30.Notably,on days 7,14 and 30,the adipogenesis of ASCs and adipose regeneration were significantly higher in the 0.075 g/ml group than in the 0.05 g/ml group(p<0.01 or p<0.001)and 0.10 g/ml group(p<0.05 or p<0.001).Conclusion:Adjusting the stiffness of DAT via physical cross-linking with MC can effectively promote adipose regeneration,which is of great significance to the development of methods for the effective repair and reconstruction of large-volume soft tissue defects. 展开更多
关键词 STIFFNESS Extracellular matrix Adipose tissue REGENERATION Adipose-derived stem cells Hydrogel STIFFNESS
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