乳腺浸润性微乳头状癌的临床病理特征及预后分析

目的:探讨乳腺浸润性微乳头状癌(invasive micropapillary carcinoma,IMPC)的临床病理特征及预后。方法:回顾性分析2011年1月至2015年12月246例于天津医科大学肿瘤医院收治的乳腺IMPC患者的临床病理资料,分为143例IMPC成分比例>50%(A组)和103例比例≤50%(B组)两组。多因素分析采用Cox比例风险回归模型,采用Log-rank检验及Kaplan Meier法等进行生存分析。结果:A组患者的5年无病生存(disease free survival,DFS)和总生存(overall survival,OS)时间均低于B组DFS(76.5%vs.83.6%,P=0.042)和OS(74.1%vs.81.6%,P=0.029)。A组中未行放疗患者的DFS和OS均低于行放疗患者的DFS(χ^2=5.219,P=0.022)和OS(χ^2=3.963,P=0.047)。Cox比例风险回归模型多因素分析显示,患者人类表皮生长因子受体-2(human epidermal growth factor receptor-2,HER-2)阳性表达(HR=2.989,95%CI:1.400~6.384,P=0.005),乳头侵犯(HR=2.388,95%CI:1.263~4.518,P=0.007),4枚以上淋巴结转移(HR=2.076,95%CI:1.080~3.992,P=0.029)为DFS独立危险因素,乳头侵犯(HR=1.951,95%CI:1.054~3.609,P=0.033)为OS独立危险因素,并且乳头侵犯中未行放疗患者的DFS和OS均低于行放疗患者的DFS(χ^2=6.541,P=0.011)和OS(χ^2=6.455,P=0.012)。结论:乳腺IMPC作为一种特殊类型乳腺癌,乳头侵犯提示预后较差。IMPC成分比例>50%较比例≤50%乳腺癌患者的预后更差,对于IMPC成分比例>50%或乳头侵犯患者,术后放疗能带来更多生存获益。

Applications,of fluorescence lifetime imaging in clinical medicine

Fluorescence lifetime is not only associated with the molecular structure f fuorophores,but alsostrongly depends on the environment around them,which llows fuorescence lifetime imagingmicroscopy(FLIM)to be used as a tool for precise measurement of the cell or tisue microenvironment,This review introduces the basic principle of fuorescence lifetime imagingtechnology and its application in clinical medicine,including research and diagnosis of diseases inskin,brain,eyes,mouth,bone,blood vessels and cavity organs,and drug evaluation.As anoninvasive,nontoxic and nonionizing radiation technique,FLIM demonstrates excellent per-formance with high sensitivity and specificity,which allows to determine precise position of thelesion and,thus,has good potential for application in biomedical research and clinical diagnosis.

Demand-vs.Supply-Driven Innovation:A Case Study of Alipay Credit Renting Platform

This study updates the source of innovation theories by investigating the relationship between demand-and supply-driven innovation through a study of a fresh Chinese case of Alipay credit renting platform.Based on the primary data collected via interview and focus group discussion,as well as the secondary data from the company’s website and media report,three main findings were obtained.First of all,from this case,we identify an unseparated relationship between demand-and supply-driven innovation and problems arose when one side is isolated and not given full attention.This adds new insights to the research on the source of innovation.Secondly,we propose that blue ocean strategical framework can help connect the demand side with the supply sides and facilitate the innovation.This provides some managerial guidelines to deal with the challenges as observed in this case.Lastly,this paper analyzes this new renting model(Alipay credit renting platform)from both lessors and lessees’perspectives,representing the first study to investigate the credit renting market systematically.

Electro-triggered Joule heating method to synthesize single-phase CuNi nano-alloy catalyst for efficient electrocatalytic nitrate reduction toward ammonia

Electrochemical nitrate reduction reaction(NO_(3)RR)has great potential for ammonia(NH_(3))synthesis benefiting from its environmental friendliness and sustainability.Cu-based alloys with elemental diversity and adsorption tunability are widely used as electrocatalyst to lower the reaction overpotential for NO_(3)RR catalysis.However,phase separation commonly found in alloys leads to uneven distribution of elements,which limits the possibility of further optimizing the catalytic activity.Herein,an electrotriggered Joule heating method,possessing unique superiority of flash heating and cooling that lead to well-dispersed nanoparticles and uniform mixing of various elements,was adopted to synthesize a single-phase CuNi nano-alloy catalyst evenly dispersed on carbon fiber paper,CFP-Cu_(1)Ni_(1),which exhibited a more positive NO_(3)RR initial potential of 0.1 V versus reversible hydrogen electrode(vs.RHE)than that of pure copper nanoparticles at 10 mA·cm^(−2)in 0.5 mol·L^(−1)Na_(2)SO_(4)+0.1 mol·L^(−1)KNO_(3)solution.Importantly,CFP-Cu_(1)Ni_(1) presented high electrocatalytic activity with a Faradaic efficiency of 95.7%and NH_(3)yield rate of 180.58μmol·h^(−1)·cm^(−2)(2550μmol·h^(−1)·mg_(cat)^(−1))at−0.22 V vs.RHE.Theoretical calculations showed that alloying Cu with Ni into single-phase caused an upshift of its d-band center,which promoted the adsorption of NO_(3)−and weakened the adsorption of NH_(3).Moreover,the competitive adsorption of hydrogen ions was restrained until−0.24 V.This work offers a rational design concept with clear guidance for rapid synthesis of uniformly dispersed single-phase nano-alloy catalyst for efficient electrochemical NO_(3)RR toward ammonia.

Event-Related Potentials Analysis on Perception of Moving Object at Different Speeds

For this research,electroencephalography(EEG)was analyzed to investigate the perception ability of the brain for moving objects at different speeds.In this experiment,total six kinds of videos regarding license plates were created,moving at distinct speed of 0.26 m/s,0.36 m/s,0.46 m/s,0.56 m/s,0.66 m/s,and 0.76 m/s,respectively.In the semantic priming paradigm,the N400 effect was analyzed for each speed.The ERP results demonstrated that the N400 amplitude gradually reduced with increasing speed.At the three lower speeds,N400 was evoked evidently and mainly distributed in the centro-posterior region.At the three higher speeds,no significant N400 effect was found.The results concluded that the perception ability of the brain declined with the acceleration of the object’s moving speed and that the brain recognized the detailed information of the moving object when its speed was lower than 0.46 m/s.

Density functional theory(DFT) studies of vanadium-titanium based selective catalytic reduction(SCR) catalysts

Based on density functional theory(DFT)and basic structure models,the chemical reactions on the surface of vanadium-titanium based selective catalytic reduction(SCR)denitrification catalysts were summarized.Reasonable structural models(non-periodic and periodic structural models)are the basis of density functional calculations.A periodic structure model was more appropriate to represent the catalyst surface,and its theoretical calculation results were more comparable with the experimental results than a nonperiodic model.It is generally believed that the SCR mechanism where NH3 and NO react to produce N2 and H2 O follows an Eley-Rideal type mechanism.NH2 NO was found to be an important intermediate in the SCR reaction,with multiple production routes.Simultaneously,the effects of H2 O,SO2 and metal on SCR catalysts were also summarized.

Analysis of oral microorganism diversity in healthy individuals before and after chewing areca nuts using PCR-denatured gradient gel electrophoresis

To analyze oral microbial diversity in the saliva of 8 healthy individuals before and after chewing areca nuts. Saliva samples were collected before chewing areca nuts, after chewing areca nuts for 5 min and after chewing areca nuts for 30 min. DNA was extracted, and microbial diversity was examined using PCR-denaturing gradient gel electrophoresis(PCR-DGGE). When examining DGGE profiles collectively,the bands associated with Streptococcus and Veillonella were the most intense, making them the most prevalent bacteria. Furthermore, the band intensities did not decrease after chewing areca nuts for 5 or30 min; thus, these bacteria were unaffected. However, when examining some individuals, the band intensities for Streptococcus and Veillonella became more intense after 5 min of chewing and then returned to the pre-chewing level. This difference may be attributed to the mechanical movements of the oral cavity or individual differences. Other bacteria, such as Neisseria, Actinomycetes, and Rothia dentocariosa, were also found to have an increased or decreased prevalence following areca nut-chewing.Since the predominant species that are present following areca nut-chewing include Streptococcus and Veillonella, it would seem likely that these bacteria play an important role in the periodontal diseases associated with areca chewing.

Construction of immobilized films photocatalysts with CdS clusters decorated by metal Cd and BiOCl for photocatalytic degradation of tetracycline antibiotics

A kind of CdS/Cd-BiOCl immobilized films photocatalyst was prepared.The optical and physicochemical properties of the CdS/Cd-BiOCl photocatalysts were analysed,and the detailed characterization revealed CdS/Cd-BiOCl films photocatalyst with good charge carrier separation effect.The reusabilities and photocatalytic properties of the samples were studied.The 15%Cd S/Cd-Bi OCl photocatalyst exhibited superior performance in photocatalytic degradation of tetracycline(TC)and favorable stability under visible light irradiation.As for the photodegradation rate of TC,15%CdS/Cd-BiOCl exhibited an excellent photodegradation activity,which is 4.06 and 9.53 times higher than that of Cd S/Cd and Bi OCl,respectively.The results showed that dominant active species are·O_(2)^(-)and·OH radicals during photodegradation.The charge transfer in Z-scheme CdS/Cd-BiOCl films photocatalyst could synchronously generate conduct band(CB)electrons in BiOCl and valence band(VB)holes in CdS,and metal Cd served as electron mediator.This work can be a reference for the design of film photocatalysts and new insight for photodegradating towards contaminants.

Enhanced performance of NiF_(2)/BiVO_(4)photoanode for photoelectrochemical water splitting

The serious surface charge recombination and fatigued photogenerated carriers transfer of the BiVO_(4)photoanode restrict its photoelectrochemical(PEC)water splitting performance.In this work,nickel fluoride(NiF_(2))is applied to revamp pure BiVO_(4)photoanode by using a facile electrodeposition method.As a result,the asprepared NiF_(2)/BiVO_(4)photoanode increases the dramatic photocurrent density by approximately 180%compared with the pristine BiVO_(4)photoanode.Furthermore,the correlative photon-to-current conversion efficiency,the charge injection,and the separation efficiency,as well as the hydrogen generation of the composite photoanode have been memorably enhanced due to the synergy of NiF_(2)and BiVO_(4).This study may furnish a dependable guidance in fabricating the fluoride-based compound/semiconductor composite photoanode system.

DNA Nanotechnology-Enabled Fabrication of Metal Nanomorphology

In recent decades,DNA nanotechnology has grown into a highly innovative and widely established field.DNA nanostructures have extraordinary structural programmability and can accurately organize nanoscale materials,especially in guiding the synthesis of metal nanomaterials,which have unique advantages in controlling the growth morphology of metal nanomaterials.This review started with the evolution in DNA nanotechnology and the types of DNA nanostructures.Next,a DNA-based nanofabrication technology,DNA metallization,was introduced.In this section,we systematically summarized the DNA-oriented synthesis of metal nanostructures with different morphologies and structures.Furthermore,the applications of metal nanostructures constructed from DNA templates in various fields including electronics,catalysis,sensing,and bioimaging were figured out.Finally,the development prospects and challenges of metal nanostructures formed under the morphology control by DNA nanotechnology were discussed.

Underground space use of urban built-up areas in the central city of Nanjing:Insight based on a dynamic population distribution

With the expansion of cities and the emergence of various urban problems,urban underground space has been developed as a solution.In China’s urban transition context,there is a need for the development of underground space in urban built-up areas.In this casestudy of the central city of Nanjing,we used spatial analysis and statistical methods to characterize the underground space use of urban built-up areas from a dynamic spatiotemporal perspective.We first analyzed the relationship between the population distribution and the underground space use of the central city of Nanjing based on a Baidu heat map,which can reflect the real-time population distribution,and then,we explored the spatiotemporal characteristics and spatial structure of the underground space use in urban built-up areas.The analysis results provide a reference for planning to improve and optimize the layout of underground space in the central city of Nanjing and,more generally,for the stock-type planning of underground space in urban built-up areas.

microRNA expression profile of fish erythrocytes

MicroRNAs(miRNAs)are short noncoding sequences that can regulate the expression of target genes.Erythrocytes,also called red blood cells(RBCs),are the largest proportion of cells in blood.Erythrocytes deliver oxygen in the body of vertebrates and contribute to the body’s immune function.Illumina sequencing technology was used to analyze pooled RNA samples isolated from erythrocytes,in order to characterize their gene expression profile and increase the range of miRNAs identified in Nile tilapia erythrocytes.This study identified conserved(n=309)and novel(n=194)miRNAs.Quantitative reverse-transcription polymerase chain reaction and next-generation sequencing results indicated that miR-451 was the most abundant in RBCs,followed by let-7a-1,miR-2188 and miR-144.Moreover,the tissue expression mode of these highly expressed miRNAs was characterized.Results show that miR-451,miR-2188,and miR-144 were all highly expressed in RBCs but were low abundance in other tissues.miR-1 and miR-10b were also abundantly expressed in muscle,while miR-122 was abundantly expressed in the liver.let-7a-1,miR-10b,and miR-1 were significantly higher in the testis than in the ovary.The tissue distribution of miRNAs implies that these noncoding sequences have a potential role in regulating tissue differentiation or maintaining tissue characteristics.The results of a dual-luciferase reporter assay indicated that miR-144 and miR-10b inhibited the expression of the meningioma expressed antigen 5 gene in vitro.This study contributes to the Nile tilapia miRNA database and reveals the profile of erythrocyte miRNA expression.

Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expression

The Lateolabrax maculatus is an important marine fish in China that can adapt to a wide range of water salinities.The gill is the main organ involved in fish osmoregulation and in this study,a cDNA library of the L.maculatus gill was constructed to understand gill function in Lateolabrax maculatus and it adaptation to salinity.A total raw data of 5.3G base pairs was obtained,followed removal of low-quality and linker DNA sequences and it yielded a total of 23,038,590 clean reads.After splicing and clustering,31,976 unigenes were obtained.The average transcript length was 1327 bp and N50 was 2057 bp.In comparisons with other tissues analysis of the gill transcriptome revealed a high level of relative expression of apolipoprotein E,CD74,keratin,type I cytoskeleton 13,carbonic anhydrase,betaine-homocysteine S-methyltransferase,and aquaporin 3(AQP3)genes.The L.maculatus aqp3(Lm-aqp3)gene was specific for the gills Lm-aqp3 and this gene has a typical transmembrane structure of six alpha helices and a water channel domain.Phylogenetic analysis indicated that Lm-aqp3 clusters with the homologue genes from Labrus bergylta and Dicentrarchus labrax.Quantitative RT-PCR confirmed that Lm-aqp3 was highly abundant in the gills and low expressed in other tissues(stomach,liver,brain,spleen and testis).Analysis of Lm-aqp3 in freshwater aquaculture fish revealed that gene expression was significantly statistical higher than in seawater(20 ppt)fish,suggesting that Lm-aqp3 may play an important role in water metabolism and osmotic pressure regulation in L.maculatus.