The ancient crop broomcorn millet(Panicum miliaceum L.)is an indispensable orphan crop in semi-arid regions due to its short life cycle and excellent abiotic stress tolerance.These advantages make it an important alte...The ancient crop broomcorn millet(Panicum miliaceum L.)is an indispensable orphan crop in semi-arid regions due to its short life cycle and excellent abiotic stress tolerance.These advantages make it an important alternative crop to increase food security and achieve the goal of zero hunger,particularly in light of the uncertainty of global climate change.However,functional genomic and biotechnological research in broomcorn millet has been hampered due to a lack of genetic tools such as transformation and genome-editing techniques.Here,we successfully performed genome editing of broomcorn millet.We identified an elite variety,Hongmi,that produces embryogenic callus and has high shoot regeneration ability in in vitro culture.We established an Agrobacterium tumefaciens-mediated genetic transformation protocol and a clustered regularly interspaced short palindromic repeats(CRISPR)/Cas9-mediated genome-editing system for Hongmi.Using these techniques,we produced herbicide-resistant transgenic plants and edited phytoene desaturase(Pm PDS),which is involved in chlorophyll biosynthesis.To facilitate the rapid adoption of Hongmi as a model line for broomcorn millet research,we assembled a near-complete genome sequence of Hongmi and comprehensively annotated its genome.Together,our results open the door to improving broomcorn millet using biotechnology.展开更多
基金funded by grants from the National Key Research and Development Program of China(2022YFD1400800,2021YFF1000101-4)the Innovation Program of the Chinese Academy of Agricultural Sciences+2 种基金the National Natural Science Foundation of China(32172093)the China Postdoctoral Science Foundation funded project(2023M743843)Initiative Scientific Research Program,Institute of Zoology,Chinese Academy of Sciences(2023IOZ0203)。
文摘The ancient crop broomcorn millet(Panicum miliaceum L.)is an indispensable orphan crop in semi-arid regions due to its short life cycle and excellent abiotic stress tolerance.These advantages make it an important alternative crop to increase food security and achieve the goal of zero hunger,particularly in light of the uncertainty of global climate change.However,functional genomic and biotechnological research in broomcorn millet has been hampered due to a lack of genetic tools such as transformation and genome-editing techniques.Here,we successfully performed genome editing of broomcorn millet.We identified an elite variety,Hongmi,that produces embryogenic callus and has high shoot regeneration ability in in vitro culture.We established an Agrobacterium tumefaciens-mediated genetic transformation protocol and a clustered regularly interspaced short palindromic repeats(CRISPR)/Cas9-mediated genome-editing system for Hongmi.Using these techniques,we produced herbicide-resistant transgenic plants and edited phytoene desaturase(Pm PDS),which is involved in chlorophyll biosynthesis.To facilitate the rapid adoption of Hongmi as a model line for broomcorn millet research,we assembled a near-complete genome sequence of Hongmi and comprehensively annotated its genome.Together,our results open the door to improving broomcorn millet using biotechnology.
