Investigation of GhFAT Genes Related to Seed Oil Content and Fatty Acid Composition in Gossypium hirsutum L.

Fatty Acyl-ACP thioesterase(FAT)is a key enzyme controlling oil biosynthesis in plant seeds.FATs can be divided into two subfamilies,FATA and FATB according to their amino acid sequences and substrate specificity.The Upland cotton genome contains 20 GhFAT genes,amongst which 6 genes were of the GhFATA subfamily and 14 of the GhFATB subfamily.The 20 GhFAT genes are unevenly distributed on 14 chromosomes.The GhFATA genes have 5 or 7 exons and the GhFATB genes have 6 or 7 exons.All GhFAT proteins have the conserved Acyl-ACP_TE domain and PLN02370 super family,the typical characteristics of plant thioesterases.Analyses of the expression level of GhFATs and the compositions of fatty acid in 5-60 days-post-anthesis seeds showed that the ratio of saturated fatty acids to unsaturated fatty acids was consistent with the expression profile of GhFATB12,GhFATB3,and GhFATB10;the ratio of monounsaturated fatty acid to polyunsaturated fatty acids was consistent with the expression profile of GhFATA3.The oil contents of mature cottonseeds were positively correlated with the contents of palmitic acid and linolenic acid as well as seed vigor.These results provide essential information for further exploring the role(s)of the specific GhFATs in determining oil biosynthesis and cottonseed compositions.

Height Detection and Analysis of Water Flowing Fractured Zone of Coal Face

Taking 91105 working face as the research object, the observation method of water flowing fractured zone and the layout of mining holes were determined by analyzing the field geological structure. It was shown that the fractured zone height and the ratio given by the measured method were 52.33 and 12.46, respectively. By the numerical simulation method with the software of UDEC, the fractured zone height and the ratio were 42.5 and 10.12. By comparison of measured height data and UDEC numerical simulation, there were some differences between the measured height and the calculated results of UDEC numerical simulation method. The method of simulation can be used as the technical basis for the design of waterproof coal pillar in the future.

Converting“cold”to“hot”:epigenetics strategies to improve immune therapy effect by regulating tumor-associated immune suppressive cells

Significant developments in cancer treatment have been made since the advent of immune therapies.However,there are still some patientswithmalignant tumors who do not benefit from immunotherapy.Tumors without immunogenicity are called“cold”tumors which are unresponsive to immunotherapy,and the opposite are“hot”tumors.Immune suppressive cells(ISCs)refer to cells which can inhibit the immune response such as tumor-associated macrophages(TAMs),myeloid-derived suppressor cells(MDSCs),regulatory T(Treg)cells and so on.The more ISCs infiltrated,the weaker the immunogenicity of the tumor,showing the characteristics of“cold”tumor.The dysfunction of ISCs in the tumor microenvironment(TME)may play essential roles in insensitive therapeutic reaction.Previous studies have found that epigeneticmechanisms play an important role in the regulation of ISCs.Regulating ISCs may be a new approach to transforming“cold”tumors into“hot”tumors.Here,we focused on the function of ISCs in the TME and discussed how epigenetics is involved in regulating ISCs.In addition,we summarized the mechanisms by which the epigenetic drugs convert immunotherapy-insensitive tumors into immunotherapy-sensitive tumors which would be an innovative tendency for future immunotherapy in“cold”tumor.

Experimental study on dense-phase pneumatic conveying of coal powder at high pressures

Clean utilization and conversion of coal resources is significant to China’s energy sustainable development.Entrained-flow coal gasification technology is an important method used for clean and efficient conversion of coal.The characteristics and stability of high-pressure dense-phase pneumatic conveying of pulverized coal is crucial to the safe and stable operation of dry-feed entrained-flow coal gasifiers.Dense-phase pneumatic conveying experiments were carried out using a high-volatile bituminous coal in pipes with diameters of 25,15 and 10 mm,respectively,and at back pressures of 1.0-4.0 MPag.The conveying characteristics and effects of operating and structure parameters were studied.Pressure drop models were established for horizontal and vertical upward conveying.The prediction uncertainty was within±30%for the horizontal conveying and±20%for the vertical upward conveying.The relative standard deviation of solid flow rate was proposed to explain conveying stability.The effect of operating parameters on conveying stability was systematically analyzed.The gas velocity-related criterion was proposed for stable conveying.