For accurately identifying the distribution charac-teristic of Gaussian-like noises in unmanned aerial vehicle(UAV)state estimation,this paper proposes a non-parametric scheme based on curve similarity matching.In the...For accurately identifying the distribution charac-teristic of Gaussian-like noises in unmanned aerial vehicle(UAV)state estimation,this paper proposes a non-parametric scheme based on curve similarity matching.In the framework of the pro-posed scheme,a Parzen window(kernel density estimation,KDE)method on sliding window technology is applied for roughly esti-mating the sample probability density,a precise data probability density function(PDF)model is constructed with the least square method on K-fold cross validation,and the testing result based on evaluation method is obtained based on some data characteristic analyses of curve shape,abruptness and symmetry.Some com-parison simulations with classical methods and UAV flight exper-iment shows that the proposed scheme has higher recognition accuracy than classical methods for some kinds of Gaussian-like data,which provides better reference for the design of Kalman filter(KF)in complex water environment.展开更多
In the present study, single factors including fermentation temperature, inoculate amount, fermentation duration, and ratio of fermentation medium volume to total flask volume(dissolved oxygen tension) were optimized ...In the present study, single factors including fermentation temperature, inoculate amount, fermentation duration, and ratio of fermentation medium volume to total flask volume(dissolved oxygen tension) were optimized for enhancing the production of coenzyme Q10 from genetic engineered Rhodobacter sphaeroides overexpressing UbiG. The experimental results suggested that optimal single factors were: inoculate amount 2%, fermentation temperature 30 ℃, fermentation duration 48 h, and ratio of fermentation medium volume to total flask volume 80%. The present study will promote the large scale production of CoQ10 from microorganisms.展开更多
This paper presents a high-performance MEMS accelerometer with a DC/AC electrostatic stiffness tuning capability based on double-sided parallel plates(DSPPs).DC and AC electrostatic tuning enable the adjustment of the...This paper presents a high-performance MEMS accelerometer with a DC/AC electrostatic stiffness tuning capability based on double-sided parallel plates(DSPPs).DC and AC electrostatic tuning enable the adjustment of the effective stiffness and the calibration of the geometric offset of the proof mass,respectively.A dynamical model of the proposed accelerometer was developed considering both DC/AC electrostatic tuning and the temperature effect.Based on the dynamical model,a self-centering closed loop is proposed for pulling the reference position of the forceto-rebalance(FTR)to the geometric center of DSPP.The self-centering accelerometer operates at the optimal reference position by eliminating the temperature drift of the readout circuit and nulling the net electrostatic tuning forces.The stiffness closed-loop is also incorporated to prevent the pull-in instability of the tuned low-stiffness accelerometer under a dramatic temperature variation.Real-time adjustments of the reference position and the DC tuning voltage are utilized to compensate for the residue temperature drift of the proposed accelerometer.As a result,a novel controlling approach composed of a self-centering closed loop,stiffness-closed loop,and temperature drift compensation is achieved for the accelerometer,realizing a temperature drift coefficient(TDC)of approximately 7μg/℃ and an Allan bias instability of less than 1μg.展开更多
This paper reports an approach of in-operation temperature bias drift compensation based on phase-based calibration for a stiffness-tunable MEMS accelerometer with double-sided parallel plate(DSPP)capacitors.The tempe...This paper reports an approach of in-operation temperature bias drift compensation based on phase-based calibration for a stiffness-tunable MEMS accelerometer with double-sided parallel plate(DSPP)capacitors.The temperature drifts of the components of the accelerometer are characterized,and analytical models are built on the basis of the measured drift results.Results reveal that the temperature drift of the acceleration output bias is dominated by the sensitive mechanical stiffness.An out-of-bandwidth AC stimulus signal is introduced to excite the accelerometer,and the interference with the acceleration measurement is minimized.The demodulated phase of the excited response exhibits a monotonic relationship with the effective stiffness of the accelerometer.Through the proposed online compensation approach,the temperature drift of the effective stiffness can be detected by the demodulated phase and compensated in real time by adjusting the stiffness-tuning voltage of DSPP capacitors.The temperature drift coefficient(TDC)of the accelerometer is reduced from 0.54 to 0.29 mg/℃,and the Allan variance bias instability of about 2.8μg is not adversely affected.Meanwhile,the pull-in resulting from the temperature drift of the effective stiffness can be prevented.TDC can be further reduced to 0.04 mg/℃through an additional offline calibration based on the demodulated carrier phase representing the temperature drift of the readout circuit.展开更多
Rhizospheres can promote self-transmissible plasmid transfer,however,the corresponding mechanism has not received much attention.Plant-microbe remediation is an effective way to promote pollutant biodegradation;howeve...Rhizospheres can promote self-transmissible plasmid transfer,however,the corresponding mechanism has not received much attention.Plant-microbe remediation is an effective way to promote pollutant biodegradation;however,some pollutants,such as naphthalene,are harmful to plants and result in inefficient plant-microbe remediation.In this study,trans-fer of a TOL-like plasmid,a self-transmissible plasmid loaded with genetic determinants for pollutant degradation,among different bacteria was examined in bulk and rhizosphere soils as well as addition of maize root exudate and its artificial root exudate(ARE).The results showed that the numbers of transconjugants and recipients as well as bacterial metabolic activities,such as xylE mRNA expression levels and catechol 2,3-dioxygenase(C23O)activ-ities of bacteria,remained high in rhizosphere soils,when compared with bulk soils.The number of transconjugants and bacterial metabolic activities increased with the increasing exudate and ARE concentrations,whereas the populations of donor and recipient bacteria were substantially unaltered at all concentrations.All the experiments consistently showed that a certain number of bacteria is required for self-transmissible plasmid transfer,and that the increased plasmid transfer might predominantly be owing to bacterial metabolic activ-ity stimulated by root exudates and ARE.Furthermore,ARE addition increased naphthalene degradation by transconjugants in both culture medium and soil.Thus,the combined action of a wide variety of components in ARE might contribute to the increased plasmid transfer and naphthalene degradation.These findings suggest that ARE could be an effectively al-ternative for plant-microbe remediation of pollutants in environments where plants cannot survive.展开更多
基金supported by the National Natural Science Foundation of China(62033010)Qing Lan Project of Jiangsu Province(R2023Q07)。
文摘For accurately identifying the distribution charac-teristic of Gaussian-like noises in unmanned aerial vehicle(UAV)state estimation,this paper proposes a non-parametric scheme based on curve similarity matching.In the framework of the pro-posed scheme,a Parzen window(kernel density estimation,KDE)method on sliding window technology is applied for roughly esti-mating the sample probability density,a precise data probability density function(PDF)model is constructed with the least square method on K-fold cross validation,and the testing result based on evaluation method is obtained based on some data characteristic analyses of curve shape,abruptness and symmetry.Some com-parison simulations with classical methods and UAV flight exper-iment shows that the proposed scheme has higher recognition accuracy than classical methods for some kinds of Gaussian-like data,which provides better reference for the design of Kalman filter(KF)in complex water environment.
基金Supported by the Project of Sichuan Science and Technology Department(2019YJ0673)National Modern Agriculture Industry System/Sichuan Live Pig Innovation Team(SCSZTD-3-007)
文摘In the present study, single factors including fermentation temperature, inoculate amount, fermentation duration, and ratio of fermentation medium volume to total flask volume(dissolved oxygen tension) were optimized for enhancing the production of coenzyme Q10 from genetic engineered Rhodobacter sphaeroides overexpressing UbiG. The experimental results suggested that optimal single factors were: inoculate amount 2%, fermentation temperature 30 ℃, fermentation duration 48 h, and ratio of fermentation medium volume to total flask volume 80%. The present study will promote the large scale production of CoQ10 from microorganisms.
基金supported by a Grant from the National Natural Science Foundation of China(Grant No.62104211).
文摘This paper presents a high-performance MEMS accelerometer with a DC/AC electrostatic stiffness tuning capability based on double-sided parallel plates(DSPPs).DC and AC electrostatic tuning enable the adjustment of the effective stiffness and the calibration of the geometric offset of the proof mass,respectively.A dynamical model of the proposed accelerometer was developed considering both DC/AC electrostatic tuning and the temperature effect.Based on the dynamical model,a self-centering closed loop is proposed for pulling the reference position of the forceto-rebalance(FTR)to the geometric center of DSPP.The self-centering accelerometer operates at the optimal reference position by eliminating the temperature drift of the readout circuit and nulling the net electrostatic tuning forces.The stiffness closed-loop is also incorporated to prevent the pull-in instability of the tuned low-stiffness accelerometer under a dramatic temperature variation.Real-time adjustments of the reference position and the DC tuning voltage are utilized to compensate for the residue temperature drift of the proposed accelerometer.As a result,a novel controlling approach composed of a self-centering closed loop,stiffness-closed loop,and temperature drift compensation is achieved for the accelerometer,realizing a temperature drift coefficient(TDC)of approximately 7μg/℃ and an Allan bias instability of less than 1μg.
基金The work is supported by the Grant of the National Natural Science Foundation of China(Grant No.62104211).
文摘This paper reports an approach of in-operation temperature bias drift compensation based on phase-based calibration for a stiffness-tunable MEMS accelerometer with double-sided parallel plate(DSPP)capacitors.The temperature drifts of the components of the accelerometer are characterized,and analytical models are built on the basis of the measured drift results.Results reveal that the temperature drift of the acceleration output bias is dominated by the sensitive mechanical stiffness.An out-of-bandwidth AC stimulus signal is introduced to excite the accelerometer,and the interference with the acceleration measurement is minimized.The demodulated phase of the excited response exhibits a monotonic relationship with the effective stiffness of the accelerometer.Through the proposed online compensation approach,the temperature drift of the effective stiffness can be detected by the demodulated phase and compensated in real time by adjusting the stiffness-tuning voltage of DSPP capacitors.The temperature drift coefficient(TDC)of the accelerometer is reduced from 0.54 to 0.29 mg/℃,and the Allan variance bias instability of about 2.8μg is not adversely affected.Meanwhile,the pull-in resulting from the temperature drift of the effective stiffness can be prevented.TDC can be further reduced to 0.04 mg/℃through an additional offline calibration based on the demodulated carrier phase representing the temperature drift of the readout circuit.
基金This work was supported by the Shanghai Municipal Science and Technology Commission(No.16391902100)the Shanghai Construction Group(No.19JCSF-12)the Ministry of Agriculture,P.R.China and Shanghai Engineering Research Center of Plant Germplasm Resources(No.17DZ2252700).
文摘Rhizospheres can promote self-transmissible plasmid transfer,however,the corresponding mechanism has not received much attention.Plant-microbe remediation is an effective way to promote pollutant biodegradation;however,some pollutants,such as naphthalene,are harmful to plants and result in inefficient plant-microbe remediation.In this study,trans-fer of a TOL-like plasmid,a self-transmissible plasmid loaded with genetic determinants for pollutant degradation,among different bacteria was examined in bulk and rhizosphere soils as well as addition of maize root exudate and its artificial root exudate(ARE).The results showed that the numbers of transconjugants and recipients as well as bacterial metabolic activities,such as xylE mRNA expression levels and catechol 2,3-dioxygenase(C23O)activ-ities of bacteria,remained high in rhizosphere soils,when compared with bulk soils.The number of transconjugants and bacterial metabolic activities increased with the increasing exudate and ARE concentrations,whereas the populations of donor and recipient bacteria were substantially unaltered at all concentrations.All the experiments consistently showed that a certain number of bacteria is required for self-transmissible plasmid transfer,and that the increased plasmid transfer might predominantly be owing to bacterial metabolic activ-ity stimulated by root exudates and ARE.Furthermore,ARE addition increased naphthalene degradation by transconjugants in both culture medium and soil.Thus,the combined action of a wide variety of components in ARE might contribute to the increased plasmid transfer and naphthalene degradation.These findings suggest that ARE could be an effectively al-ternative for plant-microbe remediation of pollutants in environments where plants cannot survive.