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Optimization of Extraction Technology of Polysaccharide of <i>Tricholoma giganteum</i>
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作者 Meihua Mo Shenghua Hu +4 位作者 Xuefeng Xu ziying ma Yan Ni Yaowu Wei Jiang Nie 《Pharmacology & Pharmacy》 2013年第5期1-5,共5页
Tricholoma giganteum also being called Tricholoma lobayense, belongs to Basidiomycotina, Hymenomycetes, Agaricales, Tricholomataceae and Tricholoma. It has high economic and medicinal values and is a kind of rare edib... Tricholoma giganteum also being called Tricholoma lobayense, belongs to Basidiomycotina, Hymenomycetes, Agaricales, Tricholomataceae and Tricholoma. It has high economic and medicinal values and is a kind of rare edible mushroom. Modern pharmacological studies have shown that T. giganteum have antitumor, antioxidant, antimicrobial and free radicals scavenging activity, and it can inhibit high blood pressure and HIV-1 reverse transcriptase. In this study, the technologies of isolation and purification of polysaccharides of T. giganteum were systematically studied. T. giganteum polysaccharides were obtained by hot-water extraction method. The infection of the extraction temperature, the extraction time, the solvent-solid ratio and the volume of solvent used during precipitation on the polysaccharides extraction ratio were studied. The extraction process was optimized by the uniform design (UD) method on the basis of the results of the single-factor experiments. The optimum extraction conditions of the mushroom polysaccharides (MP) were as follows: Extraction temperature was 100℃;extraction time was 3 hours;solvent-solid ratio was 20:1 and 5 times volume of 95% ethanol. The yield of polysaccharides of T. giganteum was 12.92%. 展开更多
关键词 POLYSACCHARIDE TRICHOLOMA giganteum EXTRACTION UNIFORM Design
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我国植物检疫性菌物数据库平台的建设及应用 被引量:2
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作者 赵鹏 段维军 +4 位作者 刘芳 周欣 范国梅 马紫英 蔡磊 《微生物学报》 CAS CSCD 北大核心 2021年第12期3806-3819,共14页
近年来,随着我国对外贸易的不断增长,口岸检测样品量巨大,外来检疫性有害生物尤其是病原菌物传入我国的风险日趋增加,正成为我国国门生物安全的重要威胁。加强外来入侵菌物的防御能力建设,有效防范其造成的生物安全威胁迫在眉睫。由于... 近年来,随着我国对外贸易的不断增长,口岸检测样品量巨大,外来检疫性有害生物尤其是病原菌物传入我国的风险日趋增加,正成为我国国门生物安全的重要威胁。加强外来入侵菌物的防御能力建设,有效防范其造成的生物安全威胁迫在眉睫。由于外来入侵菌物各类群的基础研究薄弱,标准参比物质缺乏、基础数据和可检索数据库缺失,大部分物种缺乏准确、高效的鉴定手段,使得现有口岸菌物检疫存在准确性较低、速度较慢、误检漏检率较高等问题。针对上述问题,以我国进境检疫对象及主要进口农林作物上的高频检出但鉴定困难、误检率高的菌物类群为对象,建立了其标准参比物质库、形态特征信息库、多基因序列数据库,并通过整合多个信息库资源实现从检疫样品中初筛到物种精准鉴定的多模块服务平台www.casbrc.org/pqfungi,并开放共享。该数据库平台的应用有望促进我国口岸检疫部门的检测便利化水平大幅提升,在"智能海关"建设、维护国门生物安全及促进农林产品安全贸易中发挥重要作用。 展开更多
关键词 植物检疫性菌物 标准库 多目标筛查 国门安全
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