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基于16S rDNA测序分析毛滴虫对猕猴肠道菌群结构的影响
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作者 李鹤龄 陈智岗 +3 位作者 宗发梁 宁卿 严玉霖 王宏 《中国畜牧兽医》 CAS CSCD 北大核心 2024年第11期4974-4983,共10页
【目的】通过16S rDNA测序技术分析毛滴虫感染对猕猴肠道菌群结构的影响,为临床研究利用微生物替代抗生素治疗毛滴虫病提供参考依据。【方法】分别收集毛滴虫阳性和阴性的猕猴新鲜粪便样本各6份,通过宏基因组测序进行肠道菌群和功能基... 【目的】通过16S rDNA测序技术分析毛滴虫感染对猕猴肠道菌群结构的影响,为临床研究利用微生物替代抗生素治疗毛滴虫病提供参考依据。【方法】分别收集毛滴虫阳性和阴性的猕猴新鲜粪便样本各6份,通过宏基因组测序进行肠道菌群和功能基因预测分析。【结果】与健康猕猴相比,感染毛滴虫的猕猴肠道菌群的多样性降低,菌群组成发生显著变化,拟杆菌和纤维杆菌显著减少(P<0.05),而弯曲菌和脱硫杆菌显著增加(P<0.05);肠道微生物中乳酸杆菌目(Lactobacillales)、弯曲杆菌属(Campylobacter)、弯曲杆菌科(Campylobacteraceae)等在感染毛滴虫的猕猴中显著富集,而普雷沃菌9菌(Prevotella_9)、拟杆菌目(Bacteroidales)、拟杆菌门(Bacteroidota)等在健康猕猴中更丰富;Brachyspira_hampsonii、Prevotellaceae_bacterium_Marseille_P2831是猕猴毛滴虫感染的生物标记物;肠道菌群功能基因预测显示,与氨基酸的生物合成和抗生素的生物合成途径相关的微生物基因功能在毛滴虫感染的猕猴中显著降低(P<0.05)。【结论】毛滴虫感染猕猴可降低肠道菌群的多样性,改变了肠道微生物群的结构,降低肠道菌群的生物合成功能。 展开更多
关键词 猕猴 毛滴虫 16S rDNA 肠道菌群 功能基因预测
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Survey and Analysis of Common Diseases of Laboratory Monkeys, Taking Kunming Area as an Example
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作者 Wang Hong Chen Zhigang +2 位作者 Chen Lixian Zong Faliang Li Heling 《Animal Husbandry and Feed Science》 CAS 2017年第5期289-292,321,共5页
[Objective] The paper was to understand the epidemiology of common diseases of laboratory monkeys in Kunming area. [Method]Through the investigation of sick and dead monkeys in a large-scale laboratory monkey breeding... [Objective] The paper was to understand the epidemiology of common diseases of laboratory monkeys in Kunming area. [Method]Through the investigation of sick and dead monkeys in a large-scale laboratory monkey breeding facility in Kunming area in 2014,the animals were classified depending on the main symptoms,and diseases were classified according to adult and juvenile monkeys. Data were analyzed by Office Excel 2010 software. [Result]Digestive system diseases,respiratory system diseases,trauma,reproductive system diseases,and locomotor system diseases were common disease types of laboratory monkeys in Kunming area,accounting for 47. 45%,13. 06%,12. 10%,11. 46% and 8. 92%,respectively. Common diseases included diarrhea,soft tissue injury,abortion,lobar pneumonia,dysentery,arthritis,bowel gas,chronic colitis,and extremely poor nutrition,and the ratios were 18. 47%,11. 15%,8. 91%,8. 28%,7. 96%,5. 10%,5. 10% and 5. 09%,respectively. [Conclusion]A set of scientific and reasonable measures for prevention and control of diseases is formulated,which is of great significance for improving the quantity and quality of laboratory monkeys and ensuring the accuracy of animal test results. 展开更多
关键词 Kunming area LABORATORY MONKEYS DISEASE SURVEY and ANALYSIS
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