In this paper,experimental findings concerning the kinetics of hematopoietic reconstitution are compared to corresponding clinical data.Although not clearly apparent,the transplantation practice seems to confirm the b...In this paper,experimental findings concerning the kinetics of hematopoietic reconstitution are compared to corresponding clinical data.Although not clearly apparent,the transplantation practice seems to confirm the basic proposals of experimental hematology concerning hematopoietic reconstitution resulting from successive waves of repopulation stemming from different subpopulations of progenitor and stem cells.One of the "f irst rate" parameters in clinical transplantations in hematology;i.e.the CD34+ positive cell dose,has been discussed with respect to the functional heterogeneity and variability of cell populations endowed by expression of CD34.This parameter is useful only if the relative proportion of stem and progenitor cells in the CD34+ cell population is more or less maintained in a series of patients or donors.This proportion could vary with respect to the source,pathology,treatment,processing procedure,the graft ex vivo treatment and so on.Therefore,a universal dose of CD34+ cells cannot be def ined.In addition,to avoid further confusion,the CD34+ cells should not be named "stem cells" or "progenitor cells" since these denominations only concern functionally characterized cell entities.展开更多
Recently,Niculescu published an interesting paper^(1)con-cerning“deep homologies”between the formation of cancer stem cells by polyploidization and reproductive polyploidization of entamoeba.The life cycle of both s...Recently,Niculescu published an interesting paper^(1)con-cerning“deep homologies”between the formation of cancer stem cells by polyploidization and reproductive polyploidization of entamoeba.The life cycle of both sys-tems shares the same genetic machinery inherited from the Last Eukaryote Common Ancestor(LECA).This theory claims that cancer is nothing more than the expression of a pre-existing life cycle that was reactivated in“sick and weakened metazoan cell that cannot continue its multi-cellular life and finds itself in the same dead-end situation”.展开更多
Natural killer(NK)cells are innate cytotoxic lymphoid cells(ILCs)involved in the killing of infected and tumor cells.Among human and mouse NK cells from the spleen and blood,we previously identified by single-cell RNA...Natural killer(NK)cells are innate cytotoxic lymphoid cells(ILCs)involved in the killing of infected and tumor cells.Among human and mouse NK cells from the spleen and blood,we previously identified by single-cell RNA sequencing(scRNAseq)two similar major subsets,NK1 and NK2.Using the same technology,we report here the identification,by single-cell RNA sequencing(scRNAseq),of three NK cell subpopulations in human bone marrow.Pseudotime analysis identified a subset of resident CD56^(bright) NK cells,NK0 cells,as the precursor of both circulating CD56dim NK1-like NK cells and CD56^(bright) NK2-like NK cells in human bone marrow and spleen under physiological conditions.Transcriptomic profiles of bone marrow NK cells from patients with acute myeloid leukemia(AML)exhibited stress-induced repression of NK cell effector functions,highlighting the profound impact of this disease on NK cell heterogeneity.Bone marrow NK cells from AML patients exhibited reduced levels of CD160,but the CD160high group had a significantly higher survival rate.展开更多
Correction to:Cellular&Molecular Immunology https://doi.org/10.1038/S41423-020-00574-8,published online 25 November 2020 In the version of this article initially published,two unintended errors were made during ma...Correction to:Cellular&Molecular Immunology https://doi.org/10.1038/S41423-020-00574-8,published online 25 November 2020 In the version of this article initially published,two unintended errors were made during manuscript preparation.(1)The figure legend for Panel 3B was missing;the correct legend is as follows.展开更多
文摘In this paper,experimental findings concerning the kinetics of hematopoietic reconstitution are compared to corresponding clinical data.Although not clearly apparent,the transplantation practice seems to confirm the basic proposals of experimental hematology concerning hematopoietic reconstitution resulting from successive waves of repopulation stemming from different subpopulations of progenitor and stem cells.One of the "f irst rate" parameters in clinical transplantations in hematology;i.e.the CD34+ positive cell dose,has been discussed with respect to the functional heterogeneity and variability of cell populations endowed by expression of CD34.This parameter is useful only if the relative proportion of stem and progenitor cells in the CD34+ cell population is more or less maintained in a series of patients or donors.This proportion could vary with respect to the source,pathology,treatment,processing procedure,the graft ex vivo treatment and so on.Therefore,a universal dose of CD34+ cells cannot be def ined.In addition,to avoid further confusion,the CD34+ cells should not be named "stem cells" or "progenitor cells" since these denominations only concern functionally characterized cell entities.
文摘Recently,Niculescu published an interesting paper^(1)con-cerning“deep homologies”between the formation of cancer stem cells by polyploidization and reproductive polyploidization of entamoeba.The life cycle of both sys-tems shares the same genetic machinery inherited from the Last Eukaryote Common Ancestor(LECA).This theory claims that cancer is nothing more than the expression of a pre-existing life cycle that was reactivated in“sick and weakened metazoan cell that cannot continue its multi-cellular life and finds itself in the same dead-end situation”.
基金supported by a grant from the Fondation de France(DDS Cancer 2017)P.‐Y.D.received a Fondation de France research fellowship.
文摘Natural killer(NK)cells are innate cytotoxic lymphoid cells(ILCs)involved in the killing of infected and tumor cells.Among human and mouse NK cells from the spleen and blood,we previously identified by single-cell RNA sequencing(scRNAseq)two similar major subsets,NK1 and NK2.Using the same technology,we report here the identification,by single-cell RNA sequencing(scRNAseq),of three NK cell subpopulations in human bone marrow.Pseudotime analysis identified a subset of resident CD56^(bright) NK cells,NK0 cells,as the precursor of both circulating CD56dim NK1-like NK cells and CD56^(bright) NK2-like NK cells in human bone marrow and spleen under physiological conditions.Transcriptomic profiles of bone marrow NK cells from patients with acute myeloid leukemia(AML)exhibited stress-induced repression of NK cell effector functions,highlighting the profound impact of this disease on NK cell heterogeneity.Bone marrow NK cells from AML patients exhibited reduced levels of CD160,but the CD160high group had a significantly higher survival rate.
文摘Correction to:Cellular&Molecular Immunology https://doi.org/10.1038/S41423-020-00574-8,published online 25 November 2020 In the version of this article initially published,two unintended errors were made during manuscript preparation.(1)The figure legend for Panel 3B was missing;the correct legend is as follows.
