Objective This study aimed to understand the epidemic status and phylogenetic relationships of rotavirus group A(RVA)in the Pearl River Delta region of Guangdong Province,China.Methods This study included individuals ...Objective This study aimed to understand the epidemic status and phylogenetic relationships of rotavirus group A(RVA)in the Pearl River Delta region of Guangdong Province,China.Methods This study included individuals aged 28 days–85 years.A total of 706 stool samples from patients with acute gastroenteritis collected between January 2019 and January 2020 were analyzed for 17 causative pathogens,including RVA,using a Gastrointestinal Pathogen Panel,followed by genotyping,virus isolation,and complete sequencing to assess the genetic diversity of RVA.Results The overall RVA infection rate was 14.59%(103/706),with an irregular epidemiological pattern.The proportion of co-infection with RVA and other pathogens was 39.81%(41/103).Acute gastroenteritis is highly prevalent in young children aged 0–1 year,and RVA is the key pathogen circulating in patients 6–10 months of age with diarrhea.G9P[8](58.25%,60/103)was found to be the predominant genotype in the RVA strains,and the 41 RVA-positive strains that were successfully sequenced belonged to three different RVA genotypes in the phylogenetic analysis.Recombination analysis showed that gene reassortment events,selection pressure,codon usage bias,gene polymorphism,and post-translational modifications(PTMs)occurred in the G9P[8]and G3P[8]strains.Conclusion This study provides molecular evidence of RVA prevalence in the Pearl River Delta region of China,further enriching the existing information on its genetics and evolutionary characteristics and suggesting the emergence of genetic diversity.Strengthening the surveillance of genotypic changes and gene reassortment in RVA strains is essential for further research and a better understanding of strain variations for further vaccine development.展开更多
目的基于《国际疾病分类第十一次修订本》(ICD-11)和《国际功能、残疾和健康分类》(ICF)系统综述主动式音乐疗法对认知障碍老年人认知功能的干预效果。方法构建PICO架构,采用主题词检索法,在PubMed、Web of Science、Embase、中国知网...目的基于《国际疾病分类第十一次修订本》(ICD-11)和《国际功能、残疾和健康分类》(ICF)系统综述主动式音乐疗法对认知障碍老年人认知功能的干预效果。方法构建PICO架构,采用主题词检索法,在PubMed、Web of Science、Embase、中国知网、维普和万方数据库检索采用主动式音乐疗法对认知障碍老年人认知功能进行干预的相关文献,发表时间为建库至2023年11月5日。筛选文献并提取作者、国家、发表时间、样本特征、研究方法、干预方式、测量工具和干预效果等文献特征,采用物理治疗证据数据库(PEDro)量表进行方法学质量评价,并进行系统综述。结果最终纳入8篇文献,来自6个国家。研究设计均为随机对照试验,共356例参与者,包括轻度认知障碍和痴呆老年人。文献发表时间集中在2014年至2020年,PEDro量表平均分为7.4分。所采用的干预方式为歌唱和演奏乐器。干预场所涉及医院、疗养院、健康中心。干预时长多数为每次30~60 min,少数为每次120 min。每周多为1~3次,持续8~12周。健康结局集中于认知功能方面,主要表现为改善整体认知功能、执行功能、注意功能和记忆功能。结论基于ICD-11和ICF构建了主动式音乐疗法对认知障碍老年人认知功能效益的理论架构。主动式音乐疗法有助于改善认知障碍老年人的整体认知功能、执行功能、注意功能和记忆功能。展开更多
针对当前工业类基础(industry foundation classes,IFC)标准文件的开发应用存在文件格式转换繁杂、数据互操作性弱及信息有效利用率低等问题,提出一种IFC模型数据映射至数据库、建筑信息模型(building information modeling,BIM)在Web...针对当前工业类基础(industry foundation classes,IFC)标准文件的开发应用存在文件格式转换繁杂、数据互操作性弱及信息有效利用率低等问题,提出一种IFC模型数据映射至数据库、建筑信息模型(building information modeling,BIM)在Web端重构及信息在线可视化分析的实施方法。基于Web框架,搭建IFC标准文件映射至关系型数据库的应用框架。该应用框架利用JavaScript语言对IFC标准文件深度解析,采用Python语言将IFC模型数据和建筑全生命周期有效信息储存至关系型数据库,结合实际案例及分析,展现并验证该应用框架的实施效果。结果表明:该方法可实现项目数据交互的完整性和高效性,支持建筑全生命周期相关应用的二次开发;同时融合智能建造技术和科学管理,提升BIM信息管理的有效性,促进建筑业数字化发展和智能化应用拓展。展开更多
基金funded by the grant National Key R&D Program of China(2017ZX10103011-004 and 2018YFC1603804)the Science and Technology Program of Guangdong Province(2018B020207013 and 2019B030316013).
文摘Objective This study aimed to understand the epidemic status and phylogenetic relationships of rotavirus group A(RVA)in the Pearl River Delta region of Guangdong Province,China.Methods This study included individuals aged 28 days–85 years.A total of 706 stool samples from patients with acute gastroenteritis collected between January 2019 and January 2020 were analyzed for 17 causative pathogens,including RVA,using a Gastrointestinal Pathogen Panel,followed by genotyping,virus isolation,and complete sequencing to assess the genetic diversity of RVA.Results The overall RVA infection rate was 14.59%(103/706),with an irregular epidemiological pattern.The proportion of co-infection with RVA and other pathogens was 39.81%(41/103).Acute gastroenteritis is highly prevalent in young children aged 0–1 year,and RVA is the key pathogen circulating in patients 6–10 months of age with diarrhea.G9P[8](58.25%,60/103)was found to be the predominant genotype in the RVA strains,and the 41 RVA-positive strains that were successfully sequenced belonged to three different RVA genotypes in the phylogenetic analysis.Recombination analysis showed that gene reassortment events,selection pressure,codon usage bias,gene polymorphism,and post-translational modifications(PTMs)occurred in the G9P[8]and G3P[8]strains.Conclusion This study provides molecular evidence of RVA prevalence in the Pearl River Delta region of China,further enriching the existing information on its genetics and evolutionary characteristics and suggesting the emergence of genetic diversity.Strengthening the surveillance of genotypic changes and gene reassortment in RVA strains is essential for further research and a better understanding of strain variations for further vaccine development.
文摘目的基于《国际疾病分类第十一次修订本》(ICD-11)和《国际功能、残疾和健康分类》(ICF)系统综述主动式音乐疗法对认知障碍老年人认知功能的干预效果。方法构建PICO架构,采用主题词检索法,在PubMed、Web of Science、Embase、中国知网、维普和万方数据库检索采用主动式音乐疗法对认知障碍老年人认知功能进行干预的相关文献,发表时间为建库至2023年11月5日。筛选文献并提取作者、国家、发表时间、样本特征、研究方法、干预方式、测量工具和干预效果等文献特征,采用物理治疗证据数据库(PEDro)量表进行方法学质量评价,并进行系统综述。结果最终纳入8篇文献,来自6个国家。研究设计均为随机对照试验,共356例参与者,包括轻度认知障碍和痴呆老年人。文献发表时间集中在2014年至2020年,PEDro量表平均分为7.4分。所采用的干预方式为歌唱和演奏乐器。干预场所涉及医院、疗养院、健康中心。干预时长多数为每次30~60 min,少数为每次120 min。每周多为1~3次,持续8~12周。健康结局集中于认知功能方面,主要表现为改善整体认知功能、执行功能、注意功能和记忆功能。结论基于ICD-11和ICF构建了主动式音乐疗法对认知障碍老年人认知功能效益的理论架构。主动式音乐疗法有助于改善认知障碍老年人的整体认知功能、执行功能、注意功能和记忆功能。
文摘针对当前工业类基础(industry foundation classes,IFC)标准文件的开发应用存在文件格式转换繁杂、数据互操作性弱及信息有效利用率低等问题,提出一种IFC模型数据映射至数据库、建筑信息模型(building information modeling,BIM)在Web端重构及信息在线可视化分析的实施方法。基于Web框架,搭建IFC标准文件映射至关系型数据库的应用框架。该应用框架利用JavaScript语言对IFC标准文件深度解析,采用Python语言将IFC模型数据和建筑全生命周期有效信息储存至关系型数据库,结合实际案例及分析,展现并验证该应用框架的实施效果。结果表明:该方法可实现项目数据交互的完整性和高效性,支持建筑全生命周期相关应用的二次开发;同时融合智能建造技术和科学管理,提升BIM信息管理的有效性,促进建筑业数字化发展和智能化应用拓展。
