t was found that ribonucleic acid (RNA) complexes with Tb(Ⅲ) at pH+5.0-6.5. which then emits strong characterlstlc fluorescerlstlc fluorescence of Tb(Ⅲ). The excitstion and emission wavelengths are 288nm, 4f4nm and ...t was found that ribonucleic acid (RNA) complexes with Tb(Ⅲ) at pH+5.0-6.5. which then emits strong characterlstlc fluorescerlstlc fluorescence of Tb(Ⅲ). The excitstion and emission wavelengths are 288nm, 4f4nm and 545nm, respectively.A linear relationship is obtained between the fluorescence intensity and RNA concentration in the range of 0.1μg/ml- 10 μg/ml. The detection limit is 6.0×10-8mol/L. This method can be used to determin RNA in tie presence of adenylic acid, uridylic acid and cytidylic acid.展开更多
文摘t was found that ribonucleic acid (RNA) complexes with Tb(Ⅲ) at pH+5.0-6.5. which then emits strong characterlstlc fluorescerlstlc fluorescence of Tb(Ⅲ). The excitstion and emission wavelengths are 288nm, 4f4nm and 545nm, respectively.A linear relationship is obtained between the fluorescence intensity and RNA concentration in the range of 0.1μg/ml- 10 μg/ml. The detection limit is 6.0×10-8mol/L. This method can be used to determin RNA in tie presence of adenylic acid, uridylic acid and cytidylic acid.
