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Relationship between Insuline-like Growth Factor-I and Progesterone Secretion of Cultured Human Trophoblast Cells in Vitro
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作者 Xiao-jin ZHANG Sui-qi GUI +1 位作者 Lin CAO zu-yue sun 《Journal of Reproduction and Contraception》 CAS 2007年第4期237-245,共9页
Objective To investigate the effect of insuline-like growth factor-Ⅰ (IGF-Ⅰ) on progesterone genesis and regulation. Methods Cytotrophoblast cells were collected by trypsin-collagenase digestion and percoll gradie... Objective To investigate the effect of insuline-like growth factor-Ⅰ (IGF-Ⅰ) on progesterone genesis and regulation. Methods Cytotrophoblast cells were collected by trypsin-collagenase digestion and percoll gradient centrifugation for primary culture. After stimulated with different concentrations(100 μg/ml, 10 μg/ml, 1 μg/ml, 0.1 μg/ml) of IGF-Ⅰ at the same time and with different duration(12 h,24 h,48 h, 72 h) of IGF-Ⅰ with the same concentration, progesterone levels in the media were measured by radioimmunoassay. Simultaneously, semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) was applied to determine the expression of low density lipoprotein receptor (LDLR) mRNA. Results Progesterone levels correlated positively with IGF-Ⅰ along with the IGF-Ⅰ concentration increasing, progesterone level began to increase at 12 h, and reached the climax at 48 h when cultured with 100 μg/L IGF-Ⅰ. The expression of LDLR mRNA was detectable in every group and accordant with variation of progesterone level. Conclusion Progesterone secretion has time- and dose-dependent effect on IGF-Ⅰ, and IGF-1 can up-regulate the expression of LDLR mRNA. IGF-Ⅰ may play an important role in promoting secretion of progesterone in trophoblast cells. 展开更多
关键词 trophoblast cells insuline-like growth factor-Ⅰ PROGESTERONE low density lipoprotein receptor (LDLR)
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