There is limited knowledge about microbial communities and their ecological functions in karst caves with high CO_(2)concentrations.Here,we studied the microbial community compositions and functions in Shuiming Cave( ...There is limited knowledge about microbial communities and their ecological functions in karst caves with high CO_(2)concentrations.Here,we studied the microbial community compositions and functions in Shuiming Cave( "SMC",CO_(2)concentration 3 303 ppm) and Xueyu Cave( "XYC",CO_(2)concentration 8 753 ppm) using Illumina MiSeq high-throughput sequencing in combination with BIOLOG test.The results showed that Proteobacteria,Actinobacteria and Bacteroidetes were dominant phyla in these two caves,and Thaumarchaeota was the most abundant in the rock wall samples of SMC.The microbial diversity in the water samples decreased with increasing HCO_(3)^(-)concentration,and it was higher in XYC than that in SMC.The microbial community structures in the sediment and rock wall samples were quite different between the two caves.High concentrations of CO_(2)can reduce the microbial diversity on the rock walls in karst caves,probably through changing microbial preference for different types of carbon sources and decreasing the microbial utilization rate of carbon sources.These results expanded our understanding of microbial community and its response to environments in karst caves with high CO_(2).展开更多
基金supported by the National Key Research and Development Program of China (No. 2016YFC0502501)the Special Funds for Local Science and Technology Development Guided by the Central Government, China (No. Guike ZY20198009)+2 种基金the Natural Science Foundation of Guangxi (Nos. 2015GXNSFGA139010 and 2017GXNSFBA198204)the Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences (No. 2018YT07)the Fundamental Research Funds of CAGS (No. 2020022)。
文摘There is limited knowledge about microbial communities and their ecological functions in karst caves with high CO_(2)concentrations.Here,we studied the microbial community compositions and functions in Shuiming Cave( "SMC",CO_(2)concentration 3 303 ppm) and Xueyu Cave( "XYC",CO_(2)concentration 8 753 ppm) using Illumina MiSeq high-throughput sequencing in combination with BIOLOG test.The results showed that Proteobacteria,Actinobacteria and Bacteroidetes were dominant phyla in these two caves,and Thaumarchaeota was the most abundant in the rock wall samples of SMC.The microbial diversity in the water samples decreased with increasing HCO_(3)^(-)concentration,and it was higher in XYC than that in SMC.The microbial community structures in the sediment and rock wall samples were quite different between the two caves.High concentrations of CO_(2)can reduce the microbial diversity on the rock walls in karst caves,probably through changing microbial preference for different types of carbon sources and decreasing the microbial utilization rate of carbon sources.These results expanded our understanding of microbial community and its response to environments in karst caves with high CO_(2).
