Objective To examine the precise function of influenza A virus target genes(IATGs)in malignancy.Methods Using multi-omics data from the TCGA and TCPA datasets,33 tumor types were evaluated for IATGs.IATG expression in...Objective To examine the precise function of influenza A virus target genes(IATGs)in malignancy.Methods Using multi-omics data from the TCGA and TCPA datasets,33 tumor types were evaluated for IATGs.IATG expression in cancer cells was analyzed using transcriptome analysis.Copy number variation(CNV)was assessed using GISTICS 2.0.Spearman’s analysis was used to correlate mRNA expression with methylation levels.GSEA was used for the enrichment analysis.Pearson’s correlation analysis was used to examine the association between IATG mRNA expression and IC50.The ImmuCellAI algorithm was used to calculate the infiltration scores of 24 immune cell types.Results In 13 solid tumors,IATG mRNA levels were atypically expressed.Except for UCS,UVM,KICH,PCPG,THCA,CHOL,LAMI,and MESO,most cancers contained somatic IATG mutations.The main types of CNVs in IATGs are heterozygous amplifications and deletions.In most tumors,IATG mRNA expression is adversely associated with methylation.RT-PCR demonstrated that EGFR,ANXA5,CACNA1C,CD209,UVRAG were upregulated and CLEC4M was downregulated in KIRC cell lines,consistent with the TCGA and GTEx data.Conclusion Genomic changes and clinical characteristics of IATGs were identified,which may offer fresh perspectives linking the influenza A virus to cancer.展开更多
Stomach adenocarcinoma(STAD)is one of the most common gastric neoplasms with a high death rate.Therefore,there is an urgent need to propose an efficient therapy for STAD.Copper plays key roles in regulating the distri...Stomach adenocarcinoma(STAD)is one of the most common gastric neoplasms with a high death rate.Therefore,there is an urgent need to propose an efficient therapy for STAD.Copper plays key roles in regulating the distribution of immune cells and affecting the tumor immune escape,and may be a novel indicator of immunotherapy in STAD.However,the specific impact of copper metabolism-related genes(CMRGs)on the patient’s prognosis,tumor microenvironment,and immunotherapeutic response remains unelucidated.展开更多
基金supported by the National Natural Science Foundation of China(No.82203304,82270500)Guangdong Basic and Applied Basic Research Foundation(2024B1515020113)+3 种基金High-level Hospital Construction Research Project of Maoming People’s Hospital[Yueweihan(2018)413]Science and Technology Plan Project of Maoming(No.210416154552665)Excellent Young Talent Program of Maoming People’s Hospital(NO.SY2022006)Start-up Fund of Postdoctoral Fellows to Wang Jiao Jiao(BS2021011).
文摘Objective To examine the precise function of influenza A virus target genes(IATGs)in malignancy.Methods Using multi-omics data from the TCGA and TCPA datasets,33 tumor types were evaluated for IATGs.IATG expression in cancer cells was analyzed using transcriptome analysis.Copy number variation(CNV)was assessed using GISTICS 2.0.Spearman’s analysis was used to correlate mRNA expression with methylation levels.GSEA was used for the enrichment analysis.Pearson’s correlation analysis was used to examine the association between IATG mRNA expression and IC50.The ImmuCellAI algorithm was used to calculate the infiltration scores of 24 immune cell types.Results In 13 solid tumors,IATG mRNA levels were atypically expressed.Except for UCS,UVM,KICH,PCPG,THCA,CHOL,LAMI,and MESO,most cancers contained somatic IATG mutations.The main types of CNVs in IATGs are heterozygous amplifications and deletions.In most tumors,IATG mRNA expression is adversely associated with methylation.RT-PCR demonstrated that EGFR,ANXA5,CACNA1C,CD209,UVRAG were upregulated and CLEC4M was downregulated in KIRC cell lines,consistent with the TCGA and GTEx data.Conclusion Genomic changes and clinical characteristics of IATGs were identified,which may offer fresh perspectives linking the influenza A virus to cancer.
基金the Key Discipline Construction Project of Guangdong Medical University(No.4SG22004G)the Higher Education Reform Project of Guangdong Province,China(No.2019268)+1 种基金the“Climbing Project”Special Fund for Science and Technology Innovation Cultivation of College Students in Guangdong Province,China(No.pdjh2021b0225,pdjh2022a0217)the Innovation and Entrepreneurship Training Program for students of Guangdong Medical University(No.202110571013).
文摘Stomach adenocarcinoma(STAD)is one of the most common gastric neoplasms with a high death rate.Therefore,there is an urgent need to propose an efficient therapy for STAD.Copper plays key roles in regulating the distribution of immune cells and affecting the tumor immune escape,and may be a novel indicator of immunotherapy in STAD.However,the specific impact of copper metabolism-related genes(CMRGs)on the patient’s prognosis,tumor microenvironment,and immunotherapeutic response remains unelucidated.
