A model of focal cerebral ischemic infarction was established in dogs through middle cerebral artery occlusion of the right side.Thirty minutes after occlusion,models were injected with nerve growth factor adjacent to...A model of focal cerebral ischemic infarction was established in dogs through middle cerebral artery occlusion of the right side.Thirty minutes after occlusion,models were injected with nerve growth factor adjacent to the infarct locus.The therapeutic effect of nerve growth factor against cerebral infarction was assessed using the hemisphere anomalous volume ratio,a quantitative index of diffusion-weighted MRI.At 6 hours,24 hours,7 days and 3 months after modeling,the hemisphere anomalous volume ratio was significantly reduced after treatment with nerve growth factor. Hematoxylin-eosin staining,immunohistochemistry,electron microscopy and neurological function scores showed that infarct defects were slightly reduced and neurological function significantly improved after nerve growth factor treatment.This result was consistent with diffusion-weighted MRI measurements.Experimental findings indicate that nerve growth factor can protect against cerebral infarction,and that the hemisphere anomalous volume ratio of diffusion-weighted MRI can be used to evaluate the therapeutic effect.展开更多
Radiculopathy,commonly induced by intervertebral disk bulging or protrusion,is presently diagnosed in accordance with clinical symptoms because there is no objective quantitative diagnostic criterion.Diffusion tensor ...Radiculopathy,commonly induced by intervertebral disk bulging or protrusion,is presently diagnosed in accordance with clinical symptoms because there is no objective quantitative diagnostic criterion.Diffusion tensor magnetic resonance imaging and diffusion tensor tractography revealed the characterization of anisotropic diffusion and displayed the anatomic form of nerve root fibers This study included 18 cases with intervertebral disc degeneration-induced unilateral radiculopathy Magnetic resonance diffusion tensor imaging was creatively used to reveal the scanning pattern o fiber tracking of the spinal nerve root.A scoring system of nerve root morphology was used to quantitatively assess nerve root morphology and functional alteration after intervertebral disc degeneration.Results showed that after fiber tracking,compared with unaffected nerve root,fiber bundles gathered together and interrupted at the affected side.No significant alteration was detected in the number of fiber bundles,but the cross-sectional area of nerve root fibers was reduced These results suggest that diffusion tensor magnetic resonance imaging-based tractography can be used to quantitatively evaluate nerve root function according to the area and morphology of fiber bundles of nerve roots.展开更多
基金supported by the Hebei Provincial Medical Science Research Key Youth Project,No.20100078
文摘A model of focal cerebral ischemic infarction was established in dogs through middle cerebral artery occlusion of the right side.Thirty minutes after occlusion,models were injected with nerve growth factor adjacent to the infarct locus.The therapeutic effect of nerve growth factor against cerebral infarction was assessed using the hemisphere anomalous volume ratio,a quantitative index of diffusion-weighted MRI.At 6 hours,24 hours,7 days and 3 months after modeling,the hemisphere anomalous volume ratio was significantly reduced after treatment with nerve growth factor. Hematoxylin-eosin staining,immunohistochemistry,electron microscopy and neurological function scores showed that infarct defects were slightly reduced and neurological function significantly improved after nerve growth factor treatment.This result was consistent with diffusion-weighted MRI measurements.Experimental findings indicate that nerve growth factor can protect against cerebral infarction,and that the hemisphere anomalous volume ratio of diffusion-weighted MRI can be used to evaluate the therapeutic effect.
基金supported by the Instruction Project of Health Department of Hebei Province of China in 2012,No.20120072
文摘Radiculopathy,commonly induced by intervertebral disk bulging or protrusion,is presently diagnosed in accordance with clinical symptoms because there is no objective quantitative diagnostic criterion.Diffusion tensor magnetic resonance imaging and diffusion tensor tractography revealed the characterization of anisotropic diffusion and displayed the anatomic form of nerve root fibers This study included 18 cases with intervertebral disc degeneration-induced unilateral radiculopathy Magnetic resonance diffusion tensor imaging was creatively used to reveal the scanning pattern o fiber tracking of the spinal nerve root.A scoring system of nerve root morphology was used to quantitatively assess nerve root morphology and functional alteration after intervertebral disc degeneration.Results showed that after fiber tracking,compared with unaffected nerve root,fiber bundles gathered together and interrupted at the affected side.No significant alteration was detected in the number of fiber bundles,but the cross-sectional area of nerve root fibers was reduced These results suggest that diffusion tensor magnetic resonance imaging-based tractography can be used to quantitatively evaluate nerve root function according to the area and morphology of fiber bundles of nerve roots.
