Protective effect of Danhong injection in patients with acute myocardial infarction at a high risk of no-reflow during primary percutaneous coronary intervention

Objective To observe the effect of Danhong injection(DI)in patients with acute ST-segment elevation myocardial infarction(STEMI)at a high risk of no-reflow(NR)during primary percutaneous coronary intervention(PCI).Methods Patients were placed in a DI group and control group.The DI group was given DI and the control group was given physiologic saline.The administration lasted 4 to 6 days in both groups after PCI.Cardiac magnetic resonance(CMR)was carried out during the perioperative period(7±2 days).The primary endpoint of the study was myocardial infarct size(IS)imaged on delayed-enhancement CMR.The secondary endpoint was major adverse cardiac events observed 6 months after PCI.Results In total,160 high-risk NR patients were enrolled,and 110 patients completed the CMR examination.According to postoperative CMR,the Myocardial Salvage Index and left ventricular ejection fraction were higher in the DI group(0.57±0.13 vs.0.48±0.17,P<0.01;49.3%±6.9%vs.46.2%±7.7%,P=0.03,respectively),whereas the IS was lower(19.7%±5.6%vs.22.2%±6.5%,P=0.04),compared with that in the control group.These differences were observed to be significant.After 6 months,the prevalence of major adverse cardiac events in the DI group decreased compared with that in the control group,but the differences were not observed to be significant(P>0.05).Conclusion The application of DI can reduce the myocardial infarct size in STEMI patients at a high risk of NR during primary PCI.

Genesis of green sandstone/mudstone from Middle Jurassic Zhiluo Formation in the Dongsheng Uranium Orefield, Ordos Basin and its enlightenment for uranium mineralization

The middle Jurassic Zhiluo Formation in the Dongsheng is comprised of a big set of green sandstone/mudstone with most of uranium orebodies occurring in close proximity to its footwall.By synthesizing field observations,region analysis,data collected from previous coal and uranium borehole,a regional north-south geological profile across the entire orefield is conducted.Experiments on sandstone/mudstone including rock mineral identification,clastic micromorphology and element geochemistry were carried out.Information from the geological profile indicates that green sandstone/mudstone is widely present in a stable horizon with clear boundaries to the country rock.Microscopic observations and geochemical data on sandstone/mudstone exhibit similar mineral composition with almost identical slightly flat,minor Eu enriched,Ce depleted chondrite-normalized REE patterns.Furthermore,the green clay membrane of the clasts has a complex composition containing chlorite/smectite,green smectite,chlorite,and green kaolinite,with elements including Fe,Mg,Si,and Al.These above results indicate that the green sandstone/mudstone underwent resemble sedimentary diagenetic processes as the country rock without transformation by large-scale regional fluid,while the existence of Fe2+-rich membrane is the main factor to the green sandstone/mudstone.Further concentration of the pre-enrichment uranium during diagenetic process led to the final formation for uranium deposits.The above studies are conducive to enrich the metallogenic mechanism of sandstone type uranium deposits and could provide certain reference for uranium exploration and deployment.

Two-step method for creating a gastric tube during laparoscopic-thoracoscopic Ivor-Lewis esophagectomy

AIM To introduce a two-step method for creating a gastric tube during laparoscopic-thoracoscopic Ivor-Lewis esophagectomy and assess its clinical application.METHODS One hundred and twenty-two patients with middle or lower esophageal cancer who underwent laparoscopicthoracoscopic Ivor-Lewis esophagectomy at Liaoning Cancer Hospital and Institute from March 2014 to March 2016 were included in this study,and divided into two groups based on the procedure used for creating a gastric tube. One group used a two-step method for creating a gastric tube,and the other group used the conventional method. The two groups were compared regarding the operating time,surgical complications,and number of stapler cartridges used.RESULTS The mean operating time was significantly shorter in the two-step method group than in the conventional method group [238(179-293) min vs 272(189-347) min,P < 0.01]. No postoperative death occurred in either group. There was no significant difference in the rate of complications [14(21.9%) vs 13(22.4%),P= 0.55] or mean number of stapler cartridges used [5(4-6) vs 5.2(5-6),P = 0.007] between the two groups.CONCLUSION The two-step method for creating a gastric tube during laparoscopic-thoracoscopic Ivor-Lewis esophagectomy has the advantages of simple operation,minimal damage to the tubular stomach,and reduced use of stapler cartridges.

Synergy stent for treating unprotected left main stenosis with the large reference vessel diameter

For many years,unprotected left main stenosis(ULMS)has been remained as the forbidden fruit of interventional cardiology.However,with the remarkable improvement in medical device technology,procedural techniques and antithrombotic agents during the last decades,percutaneous coronary intervention(PCI)with stenting for ULMS has become technically feasible and has exhibited favorable clinical outcomes.

Mutation screening of mismatch repair gene Mlh3 in familial esophageal cancer

AIM： To shed light on the possible role of mismatch repair gene MIh3 in familial esophageal cancer （FEC）. METHODS： A total of 66 members from 10 families suggestive of a genetic predisposition to hereditary esophageal cancer were screened for germline mutations in MIh3 with denaturing high performance liquid chromatography （DHPLC）, a newly developed method of comparative sequencing based on heteroduplex detection. For all samples exhibiting abnormal DHPLC profiles, sequence changes were evaluated by cycle sequencing. For any mutation in family members, we conducted a segregation study to compare its prevalence in sporadic esophageal cancer patients and normal controls. RESULTS： Exons of MIh3 in all samples were successfully examined. Overall, 4 missense mutations and 3 polymorphisms were identified in 4 families. MIh3 missense mutations in families 9 and 10 might be pathogenic, but had a reduced penetrance. While in families 1 and 7, there was no sufficient evidence supporting the monogenic explanations of esophageal cancers in families. The mutations were found in 33% of high-risk families and 50% of low-risk families.CONCLUSION： MIh3 is a high risk gene with a reduced penetrance in some families. However, it acts as a low risk gene for esophageal cancer in most families. Mutations of MIh3 may work together with other genes in an accumulated manner and result in an increased risk of esophageal tumor. DHPLC is a robust and sensitive technique for screening gene mutations.

Lyophilized lymph nodes:A paradigm shift in CAR T-cell delivery for solid tumor therapy

CAR T(chimeric antigen receptor T-cell)therapy represents a paradigm shift in cancer treatments.By empowering immune cells to target malignant cells directly,it opens another door to precision medicine,promising cures for once refractory malignancies.However,the extension of CAR T therapy to solid tumors confronts formidable obstacles1.The physical and biochemical barriers within the tumor microenvironment,such as dense extracellular matrices and immunosuppressive factors,impede CAR T cell infiltration and function,leading to diminished success rates in solid tumor treatment2.

YB1 regulates miR-205/200b-ZEB1 axis by inhibiting microRNA maturation in hepatocellular carcinoma

Background:Y-box binding protein 1(YB1 or YBX1)plays a critical role in tumorigenesis and cancer progression.However,whether YB1 affects malignant transformation by modulating non-codingRNAs remains largely unknown.This study aimed to investigate the relationship between YB1 and microRNAs and reveal the underlying mechanism by which YB1 impacts on tumor malignancy via miRNAs-mediated regulatory network.Methods:The biological functions of YB1 in hepatocellular carcinoma(HCC)cells were investigated by cell proliferation,wound healing,and transwell invasion assays.The miRNAs dysregulated by YB1 were screened by microarray analysis in HCC cell lines.The regulation of YB1 on miR-205 and miR-200b was determined by quantitative real-time PCR,dual-luciferase reporter assay,RNA immunoprecipitation,and pull-down assay.The relationships of YB1,DGCR8,Dicer,TUT4,and TUT1 were identified by pull-down and coimmunoprecipitation experiments.The cellular co-localization of YB1,DGCR8,and Dicer were detected by immunofluorescent staining.The in vivo effect of YB1 on tumor metastasis was determined by injecting MHCC97H cells transduced with YB1 shRNA or shControl via the tail vein in nude BALB/c mice.The expression levels of epithelial tomesenchymal transition markerswere detected by immunoblotting and immunohistochemistry assays.Results:YB1 promoted HCC cell migration and tumor metastasis by regulating miR-205/200b‒ZEB1 axis partially in a Snail-independent manner.YB1 suppressedmiR-205 and miR-200b maturation by interacting with the microprocessors DGCR8 and Dicer as well as TUT4 and TUT1 via the conserved cold shock domain.Subsequently,the downregulation of miR-205 and miR-200b enhanced ZEB1 expression,thus leading to increased cell migration and invasion.Furthermore,statistical analyses on gene expression data from HCC and normal liver tissues showed that YB1 expression was positively associated with ZEB1 expression and remarkably correlated with clinical prognosis.Conclusion:This study reveals a previously undescribed mechanism by which YB1 promotes cancer progression by regulating the miR-205/200b‒ZEB1 axis in HCC cells.Furthermore,these results highlight that YB1 may play biological functions via miRNAs-mediated gene regulation,and it can serve as a potential therapeutic target in human cancers.

Danhong Injection Improves Elective Percutaneous Coronary Intervention in UA Patients with Blood Stasis Syndrome Revealed by Perioperative Metabolomics

Objective:To observe the effect of Danhong injection(DHI)on perioperative metabolomics of unstable angina pectoris(UA)with blood stasis syndrome.Materials and Methods:A prospective,randomized,controlled,and single-blind clinical trial was conducted.Sixty-one UA patients with traditional Chinese medicine blood stasis syndrome undergoing elective percutaneous coronary intervention(PCI)were randomly divided into the Danhong and control groups,and 10 healthy volunteers were included as baseline.The Danhong group received western medicine+DHI treatment,while the control group received western medicine+saline.Nontargeted metabolomics was used to analyze the serum metabolites of healthy volunteers in the Danhong and control groups before and 5 days after PCI.Results:Before treatment,there was no significant difference in serum metabolites between the Danhong and control groups,but there was a significant difference between the two groups and the healthy group.Differential metabolites were clustered mainly in glycerophospholipid,sphingolipid,purine,and amino acid groups,which were generated in their metabolic pathways.After 5 days of PCI,the profiles of serum metabolites were significantly closer between the Danhong-or control-treated groups and that of the healthy group.Furthermore,DHI treatment converted the serum metabolite profile more to that of the healthy group than the control treatment.Conclusion:The beneficial effect of DHI on patients with unstable angina is reflected at the level of serum metabolic biomarkers.

A solid phase approach to PDMP analogs: A general strategy for combinatorial library

The present work reports the first solid phase synthesis of biologically interesting D-threo-l-phenyl-2- decanoylamino-3-morpholino-l-propanol （D-threo-PDMP） derivatives. This synthetic strategy includes facile preparation of versatile azido intermediate （5） in a relatively short sequence and the subsequent derivatization of 5, which led to a series of sulfonamide, urea and heterocycle substituted PDMP analogs （10 and 10＇）. With this method, a 5280-member compound library has been successfully built by IRORI Nanokan system.