Background:In recent years,day surgery has gained widespread attention in the medical industry.This thesis focuses on three tertiary general hospitals in different regions in China where day surgery has been carried o...Background:In recent years,day surgery has gained widespread attention in the medical industry.This thesis focuses on three tertiary general hospitals in different regions in China where day surgery has been carried out earlier,is more developed,has a more comprehensive range of day surgery procedures,and has an annual volume of 10,000 or more day surgery cases.The study will provide hands-on experience for other medical institutions to carry out day surgery.Methods:67,337 valid items of data were retrieved from the Hospital Information Systems of the three hospitals.In this thesis,descriptive analysis was carried out on basic information about day surgery patients,including composition of patients’major diseases,hospitalization costs.Non-parametric tests were used to compare the data in each group,the median and interquartile range were used to show the trend in the concentration of the relevant indicators.The difference was considered statistically significant at P<0.05.Results:The largest proportion of day surgery patients were between the ages of 51 and 70.About 57.30%of day surgery patients paid for their medical care with the basic medical insurance of urban employees.Senile cataract,benign breast tumor,and endometrial polyp were the top three major diagnoses of day surgery patients.The average inpatient expenditure for day surgery patients was 8982.10 yuan,with consumables cost representing the highest proportion at approximately 46.01%of the total hospitalization cost,followed by treatment cost at around 31.01%of the total cost.Conclusions:Day surgery is more suitable for middle-aged patients and may not be the best choice for children and elderly patients over 80 years old.Most day surgery cases are elective surgeries for non-emergency situations.The distribution of day surgery patients is primarily concentrated in ophthalmic,breast,and reproductive system diseases,although each hospital has its own unique characteristics.The development of day surgery presents both economic and social benefits.展开更多
Boreal forests commonly suff er from nitrogen defi ciency due to low rate of nitrogen mineralization.Biochar may promote soil organic matter decomposition and accelerate nitrogen mineralization.In this study,Illumina ...Boreal forests commonly suff er from nitrogen defi ciency due to low rate of nitrogen mineralization.Biochar may promote soil organic matter decomposition and accelerate nitrogen mineralization.In this study,Illumina NovaSeq sequencing combined with functional annotation of prokaryotic taxa(FAPROTAX)analysis was used to investigate the eff ect of biochar pyrolysis temperatures,the amount of applied biochar,and the period since the biochar application(2-and 3-year)on soil bacterial communities.The results show that biochar pyrolysis temperatures(500℃ and 650℃)and the amount of applied biochar(0.5 kg m^(−2)and 1.0 kg m^(−2))did not change soil properties.Nevertheless,the interaction of biochar pyrolysis temperature and the amount had signifi cant eff ects on bacterial species richness and evenness(P<0.05).The application of biochar produced at 500℃ had a lower abundance of Actinobacteria and Verrucomicrobia,while that produced at 650℃ had a higher abundance of Conexibacter and Phenylobacterium.When biochar produced at 650℃ was applied,applying 0.5 kg m^(−2)had a higher abundance of Cyanobacteria,Conexibacter,and Phenylobacterium than that of 1.0 kg m^(−2)(P<0.05).Functionally,the abundance of the aromatic compound degradation group increased with the extension of application time and increase of pyrolysis temperature.The time since application played an important role in the formation of soil bacterial communities and their functional structure.Long-term studies are necessary to understand the consequence of biochar on bacterial communities in boreal forests.展开更多
Background:Benign prostatic hyperplasia(BPH)is a common condition in middle-aged and elderly men.Enlargement of the prostate causes lower urinary tract symptoms.Capsaicin is a phytochemical extracted from chili pepper...Background:Benign prostatic hyperplasia(BPH)is a common condition in middle-aged and elderly men.Enlargement of the prostate causes lower urinary tract symptoms.Capsaicin is a phytochemical extracted from chili peppers and exerts many pharmacological actions,such as anti-tumor and anti-inflammatory effects.Methods:Our study investigated the effect of capsaicin in vitro and in a mouse model in vivo.A prostatic stromal myofibroblast cell line(WPMY-1)was co-incubated with testosterone(1µM)and different concentrations of capsaicin(10–100µM)for 24 and 48 h.Capsaicin(10–100µM)significantly inhibited testosterone-treated WPMY-1 cell growth at 48 h by MTT assay.The testosterone propionate(7.5 mg/kg)-induced BPH mouse model was used to examine the anti-proliferative effect of capsaicin.Treatment with capsaicin(10 mg/kg)for 14 days significantly attenuated prostatic hyperplasia.Finasteride was used as a positive control.Results:Capsaicin significantly decreased prostate weight and prostate index(prostate/body weight ratio)in BPH mice.The expression of 5α-reductase type II,androgen receptor(AR)and prostate specific antigen(PSA)protein expression and PSA serum were all significantly reduced in capsaicin-treated BPH mice.In addition,capsaicin also activated transient receptor potential vanilloid 1 mediated apoptosis and autophagy in BPH mice.Conclusion:These results demonstrate multiple positive effects of capsaicin in controlling prostate growth and suggest its therapeutic potential in the treatment of BPH.展开更多
BACKGROUND:This study aimed to explore the effects of TNF-a on the expression of IP_3R1mRNA and protein in human mesangial cells(HMCs),and to elucidate the mechanism of TNF-a relating to IP_3R1 expression in the occur...BACKGROUND:This study aimed to explore the effects of TNF-a on the expression of IP_3R1mRNA and protein in human mesangial cells(HMCs),and to elucidate the mechanism of TNF-a relating to IP_3R1 expression in the occurrence of hepatorenal syndrome(HRS).METHODS:HMCs were stimulated by tumor(TNF-a) with 100 ng/mL for different hours(2,4,8,and 24 hours).The expression changes of IP_3R1 mRNA and protein were detected by quantitative real-time polymerase chain reaction and immunoblotting.Several inhibitors including D609,U73122,PP1,safingol,rottlerin and non-radioactive protein kinase C(PKC) were used to examine the mechanism of signal transduction ofTNF-a-regulated IP_3R1 in HMCs.RESULTS:The levels of IP_3R1 mRNA at 2 hours after TNF-a exposure were significantly enhanced and peaked at 8 hours in HMCs(P<0.01),then descended at 24 hours(P<0.01).The levels of IP_3R1 protein at 4 hours after TNF-a exposure were obviously increased and peaked at24 hours after TNF-a exposure(P<0.01).Compared to the control group,safingol(PKCa inhibitor)and D609(phosphatidylcholine-specific phospholipase C inhibitor) significantly blocked the TNF-ainduced expression of IP_3R1 mRNA(3.30±0.81 vs.1.95±0.13,P<0.05;2.10±0.49,P<0.01) and IP_3R1protein(3.09±0.13 vs.1.86+0.39,P<0.01;1.98±0.02,P<0.01).TNF-a promoted PKCa activation with maximal PKCa phosphorylation that occurred 8 hours after stimulation measured by non-radioactive PKC assay,and the effect was markedly attenuated by pretreatment with D609 or safingol.CONCLUSION:TNF-a increased the expression of IP_3R1 and this was mediated,at least in part,through the PC-PLC/PKCa signaling pathways in HMCs.展开更多
Seasonally frozen soil in alpine and subalpine zones in the mountains of Qinghai-Tibetan Plateau is particularly sensitive to global climate change. Therefore, a better understanding of the thermal properties of froze...Seasonally frozen soil in alpine and subalpine zones in the mountains of Qinghai-Tibetan Plateau is particularly sensitive to global climate change. Therefore, a better understanding of the thermal properties of frozen soil is crucial for predicting the responses of frozen soils to soil warming. In this study, thermal properties of frozen soil with different moisture contents under subzero temperature (0°C - 20°C) in an alpine forest in western Sichuan were analyzed by KD<sub>2</sub> Pro in its cooling and heating processes, respectively. Our results reveal that the soil apparent volumetric specific heat capacity (C<sub>v</sub>) and apparent thermal conductivity (K) under the same water content show similar response patterns to changing temperature lower than -2°C in both heating and cooling processes. Moreover, ice content of frozen soils can be well predicted by Logistic model in cooling and heating processes. The C<sub>v</sub> and K tend to increase along with increasing soil moisture contents. Remarkably, asymptotic characters of the value of C<sub>v</sub> and K are at the vicinity of the initial temperature of phase transitions, indicating that both C<sub>v</sub> and K are particularly sensitive to changing soil temperature at the range of -2°C to 0°C. Therefore, the widely distributed frozen soil layers with temperature above -2°C in alpine and subalpine zones over Qinghai-Tibetan Plateau are susceptible to the observed climate warming during cold season.展开更多
AIM:To investigate the risk factors for gallstone disease in the general population of Chengdu,China. METHODS:This study was conducted at the West China Hospital.Subjects who received a physical examination at this ho...AIM:To investigate the risk factors for gallstone disease in the general population of Chengdu,China. METHODS:This study was conducted at the West China Hospital.Subjects who received a physical examination at this hospital between January and December 2007 were included.Body mass index,blood pressure,fasting plasma glucose,serum lipid and lipoproteins concentrations were analyzed.Gallstone disease was diagnosed by ultrasound or on the basis of a history of cholecystectomy because of gallstone disease.Unconditional logistic regression analysis was used to investigate the risk factors for gallstone disease,and the Chi-square test was used to analyze differences in the incidence of metabolic disorders between subjects with and without gallstone disease. RESULTS:A total of 3573 people were included, 10.7%(384/3573)of whom had gallstone diseases. Multiple logistic regression analysis indicated that the incidence of gallstone disease in subjects aged 40-64 or≥65 years was significantly different from that in those aged 18-39 years(P<0.05);the incidence was higher in women than in men(P<0.05).In men,a high level of fasting plasma glucose was obvious in gallstone disease(P<0.05),and in women, hypertriglyceridemia or obesity were significant in gallstone disease(P<0.05).展开更多
Evaluation of the efficacy of traditional Chinese medicines (TCMs) is an important prerequisite for discovering effective substances, lead compounds, and quality markers (Q markers). At present, there is an urgent nee...Evaluation of the efficacy of traditional Chinese medicines (TCMs) is an important prerequisite for discovering effective substances, lead compounds, and quality markers (Q markers). At present, there is an urgent need to develop a biological language that can act as abridge for the scientific elaboration of the efficacy of TCMs, and to further highlight the significant value of TCM. Chinese medicinal syndromes and formulae are two essential parts of TCM that directly relate to its efficacy. Syndromes and formulae have been taken as the research objects. The serum pharmacochemistry of the TCM approach with metabolomics were integrated to establish an innovative chinmedomics strategy, which is able to explore syndrome biomarkers and evaluate TCM efficacy in order to discover effective substances from TCMs. A great deal of concrete work in chinmedomics has already performed to bridge the gap between Chinese and Western medicine, and to provide a powerful approach to enhance the scientific value of TCM theory and clinical practice. This article summarizes the application of chinmedomics in identifying the candidate biomarkers of a syndrome and revealing the efficacy of the related formula. We also highlight the discovery of lead compounds and Q markers from TCMs.展开更多
基金supported by the National Natural Science Foundation of China(No.51627802)the Inner Mongolia Science and Technology Revitalization Project,China(No.XM2020BT14)。
基金supporting data analysis related to the patient experience survey。
文摘Background:In recent years,day surgery has gained widespread attention in the medical industry.This thesis focuses on three tertiary general hospitals in different regions in China where day surgery has been carried out earlier,is more developed,has a more comprehensive range of day surgery procedures,and has an annual volume of 10,000 or more day surgery cases.The study will provide hands-on experience for other medical institutions to carry out day surgery.Methods:67,337 valid items of data were retrieved from the Hospital Information Systems of the three hospitals.In this thesis,descriptive analysis was carried out on basic information about day surgery patients,including composition of patients’major diseases,hospitalization costs.Non-parametric tests were used to compare the data in each group,the median and interquartile range were used to show the trend in the concentration of the relevant indicators.The difference was considered statistically significant at P<0.05.Results:The largest proportion of day surgery patients were between the ages of 51 and 70.About 57.30%of day surgery patients paid for their medical care with the basic medical insurance of urban employees.Senile cataract,benign breast tumor,and endometrial polyp were the top three major diagnoses of day surgery patients.The average inpatient expenditure for day surgery patients was 8982.10 yuan,with consumables cost representing the highest proportion at approximately 46.01%of the total hospitalization cost,followed by treatment cost at around 31.01%of the total cost.Conclusions:Day surgery is more suitable for middle-aged patients and may not be the best choice for children and elderly patients over 80 years old.Most day surgery cases are elective surgeries for non-emergency situations.The distribution of day surgery patients is primarily concentrated in ophthalmic,breast,and reproductive system diseases,although each hospital has its own unique characteristics.The development of day surgery presents both economic and social benefits.
基金This work was supported by key projects of National Natural Science Foundation of China(No.81830110)Heilongjiang Province key research and development plan(No.2022ZX02C04).
基金funded by The Foundation for Research of Natural Resources in Finland(2016085)supported by the Academy of Finland(286685,294600,307222,277623)the FCoE of atmospheric sciences(Center of Excellence(1118615)).
文摘Boreal forests commonly suff er from nitrogen defi ciency due to low rate of nitrogen mineralization.Biochar may promote soil organic matter decomposition and accelerate nitrogen mineralization.In this study,Illumina NovaSeq sequencing combined with functional annotation of prokaryotic taxa(FAPROTAX)analysis was used to investigate the eff ect of biochar pyrolysis temperatures,the amount of applied biochar,and the period since the biochar application(2-and 3-year)on soil bacterial communities.The results show that biochar pyrolysis temperatures(500℃ and 650℃)and the amount of applied biochar(0.5 kg m^(−2)and 1.0 kg m^(−2))did not change soil properties.Nevertheless,the interaction of biochar pyrolysis temperature and the amount had signifi cant eff ects on bacterial species richness and evenness(P<0.05).The application of biochar produced at 500℃ had a lower abundance of Actinobacteria and Verrucomicrobia,while that produced at 650℃ had a higher abundance of Conexibacter and Phenylobacterium.When biochar produced at 650℃ was applied,applying 0.5 kg m^(−2)had a higher abundance of Cyanobacteria,Conexibacter,and Phenylobacterium than that of 1.0 kg m^(−2)(P<0.05).Functionally,the abundance of the aromatic compound degradation group increased with the extension of application time and increase of pyrolysis temperature.The time since application played an important role in the formation of soil bacterial communities and their functional structure.Long-term studies are necessary to understand the consequence of biochar on bacterial communities in boreal forests.
基金Yichun University Local Development Research Center(Grant No.DF2019002)the PhD Research Foundation of Yichun University(Grant No.211-3360118006)the Animal Care and Ethics Committee of Yichun University(Approval No.2022026).
文摘Background:Benign prostatic hyperplasia(BPH)is a common condition in middle-aged and elderly men.Enlargement of the prostate causes lower urinary tract symptoms.Capsaicin is a phytochemical extracted from chili peppers and exerts many pharmacological actions,such as anti-tumor and anti-inflammatory effects.Methods:Our study investigated the effect of capsaicin in vitro and in a mouse model in vivo.A prostatic stromal myofibroblast cell line(WPMY-1)was co-incubated with testosterone(1µM)and different concentrations of capsaicin(10–100µM)for 24 and 48 h.Capsaicin(10–100µM)significantly inhibited testosterone-treated WPMY-1 cell growth at 48 h by MTT assay.The testosterone propionate(7.5 mg/kg)-induced BPH mouse model was used to examine the anti-proliferative effect of capsaicin.Treatment with capsaicin(10 mg/kg)for 14 days significantly attenuated prostatic hyperplasia.Finasteride was used as a positive control.Results:Capsaicin significantly decreased prostate weight and prostate index(prostate/body weight ratio)in BPH mice.The expression of 5α-reductase type II,androgen receptor(AR)and prostate specific antigen(PSA)protein expression and PSA serum were all significantly reduced in capsaicin-treated BPH mice.In addition,capsaicin also activated transient receptor potential vanilloid 1 mediated apoptosis and autophagy in BPH mice.Conclusion:These results demonstrate multiple positive effects of capsaicin in controlling prostate growth and suggest its therapeutic potential in the treatment of BPH.
基金supported by a grant from Health Bureauof Jiangxi Province
文摘BACKGROUND:This study aimed to explore the effects of TNF-a on the expression of IP_3R1mRNA and protein in human mesangial cells(HMCs),and to elucidate the mechanism of TNF-a relating to IP_3R1 expression in the occurrence of hepatorenal syndrome(HRS).METHODS:HMCs were stimulated by tumor(TNF-a) with 100 ng/mL for different hours(2,4,8,and 24 hours).The expression changes of IP_3R1 mRNA and protein were detected by quantitative real-time polymerase chain reaction and immunoblotting.Several inhibitors including D609,U73122,PP1,safingol,rottlerin and non-radioactive protein kinase C(PKC) were used to examine the mechanism of signal transduction ofTNF-a-regulated IP_3R1 in HMCs.RESULTS:The levels of IP_3R1 mRNA at 2 hours after TNF-a exposure were significantly enhanced and peaked at 8 hours in HMCs(P<0.01),then descended at 24 hours(P<0.01).The levels of IP_3R1 protein at 4 hours after TNF-a exposure were obviously increased and peaked at24 hours after TNF-a exposure(P<0.01).Compared to the control group,safingol(PKCa inhibitor)and D609(phosphatidylcholine-specific phospholipase C inhibitor) significantly blocked the TNF-ainduced expression of IP_3R1 mRNA(3.30±0.81 vs.1.95±0.13,P<0.05;2.10±0.49,P<0.01) and IP_3R1protein(3.09±0.13 vs.1.86+0.39,P<0.01;1.98±0.02,P<0.01).TNF-a promoted PKCa activation with maximal PKCa phosphorylation that occurred 8 hours after stimulation measured by non-radioactive PKC assay,and the effect was markedly attenuated by pretreatment with D609 or safingol.CONCLUSION:TNF-a increased the expression of IP_3R1 and this was mediated,at least in part,through the PC-PLC/PKCa signaling pathways in HMCs.
文摘Seasonally frozen soil in alpine and subalpine zones in the mountains of Qinghai-Tibetan Plateau is particularly sensitive to global climate change. Therefore, a better understanding of the thermal properties of frozen soil is crucial for predicting the responses of frozen soils to soil warming. In this study, thermal properties of frozen soil with different moisture contents under subzero temperature (0°C - 20°C) in an alpine forest in western Sichuan were analyzed by KD<sub>2</sub> Pro in its cooling and heating processes, respectively. Our results reveal that the soil apparent volumetric specific heat capacity (C<sub>v</sub>) and apparent thermal conductivity (K) under the same water content show similar response patterns to changing temperature lower than -2°C in both heating and cooling processes. Moreover, ice content of frozen soils can be well predicted by Logistic model in cooling and heating processes. The C<sub>v</sub> and K tend to increase along with increasing soil moisture contents. Remarkably, asymptotic characters of the value of C<sub>v</sub> and K are at the vicinity of the initial temperature of phase transitions, indicating that both C<sub>v</sub> and K are particularly sensitive to changing soil temperature at the range of -2°C to 0°C. Therefore, the widely distributed frozen soil layers with temperature above -2°C in alpine and subalpine zones over Qinghai-Tibetan Plateau are susceptible to the observed climate warming during cold season.
基金The National Natural Science Foundation of China,No.30571640the National Basic Research Program of China,No.2006CB504302 and No.2007CB512902
文摘AIM:To investigate the risk factors for gallstone disease in the general population of Chengdu,China. METHODS:This study was conducted at the West China Hospital.Subjects who received a physical examination at this hospital between January and December 2007 were included.Body mass index,blood pressure,fasting plasma glucose,serum lipid and lipoproteins concentrations were analyzed.Gallstone disease was diagnosed by ultrasound or on the basis of a history of cholecystectomy because of gallstone disease.Unconditional logistic regression analysis was used to investigate the risk factors for gallstone disease,and the Chi-square test was used to analyze differences in the incidence of metabolic disorders between subjects with and without gallstone disease. RESULTS:A total of 3573 people were included, 10.7%(384/3573)of whom had gallstone diseases. Multiple logistic regression analysis indicated that the incidence of gallstone disease in subjects aged 40-64 or≥65 years was significantly different from that in those aged 18-39 years(P<0.05);the incidence was higher in women than in men(P<0.05).In men,a high level of fasting plasma glucose was obvious in gallstone disease(P<0.05),and in women, hypertriglyceridemia or obesity were significant in gallstone disease(P<0.05).
基金grants from the Key Program of National Natural Science Foundation of China (81830110, 81430093, 81373930, 81673586, 81302905, and 81503386)the National Key Subject of Drug Innovation (2015ZX09101043-005 and 2015ZX09101043-011)+2 种基金the TCM State Administration Subject of Public Welfare (2015468004)the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (UNPYSCT-2015118)the Young Talent Lift Engineering Project of China Association of Traditional Chinese Medicine (QNRC2-B06).
文摘Evaluation of the efficacy of traditional Chinese medicines (TCMs) is an important prerequisite for discovering effective substances, lead compounds, and quality markers (Q markers). At present, there is an urgent need to develop a biological language that can act as abridge for the scientific elaboration of the efficacy of TCMs, and to further highlight the significant value of TCM. Chinese medicinal syndromes and formulae are two essential parts of TCM that directly relate to its efficacy. Syndromes and formulae have been taken as the research objects. The serum pharmacochemistry of the TCM approach with metabolomics were integrated to establish an innovative chinmedomics strategy, which is able to explore syndrome biomarkers and evaluate TCM efficacy in order to discover effective substances from TCMs. A great deal of concrete work in chinmedomics has already performed to bridge the gap between Chinese and Western medicine, and to provide a powerful approach to enhance the scientific value of TCM theory and clinical practice. This article summarizes the application of chinmedomics in identifying the candidate biomarkers of a syndrome and revealing the efficacy of the related formula. We also highlight the discovery of lead compounds and Q markers from TCMs.
