The treatment of shavings,trimmings and splits of leather waste from tanneries has a potential to generate value-added products. In this study enzymatic treatment of leather waste was performed. This method utilizes a...The treatment of shavings,trimmings and splits of leather waste from tanneries has a potential to generate value-added products. In this study enzymatic treatment of leather waste was performed. This method utilizes alkaline protease produced by Bacillus subtilis in our laboratory by submerged fermentation. Optimum conditions of pH,time duration,temperature and concentration of enzyme were determined for maximum degradation of leather waste. The amount of degradation was measured by the release of amino acid hydroxyproline. Amino acid composition in the hydrolysate obtained by the enzyme hydrolysis was determined. This relative simple biotreatment of leather waste may provide a practical and economical solution.展开更多
The present study is concerned with the isolation and screening of different strains of Aspergillus oryzae for the production of alpha amylase. Ninety strains were isolated from soil and tested for the production of a...The present study is concerned with the isolation and screening of different strains of Aspergillus oryzae for the production of alpha amylase. Ninety strains were isolated from soil and tested for the production of alpha amylase in shake flasks. Of all the strains tested, Aspergillus oryzae GCB-32 and Aspergillus oryzae GCB-35 gave maximum production of alpha amylase. Different culture media were screened for the production of alpha amylase by these two strains. M1 medium containing starch, yeast extract, NH4Cl, MgSO4·7H2O and CaCl2 gave the maximum production of alpha amylase by both the strains Aspergillus oryzae GCB-32 and Aspergillus oryzae GCB-35.Kinetic analysis revealed that the values of product yield coefficient（Y p/x ） and specific product yield coefficient（qp） were found highly significant（p≤0.05） when medium M1 was used for the enzyme production.展开更多
The okra germplasm was screened for salinity tolerance at the seedling stage and during plant ontogeny.Substantial variation existed in okra for salinity tolerance at the seedling stage.An 80 mmol/L NaCl concentration...The okra germplasm was screened for salinity tolerance at the seedling stage and during plant ontogeny.Substantial variation existed in okra for salinity tolerance at the seedling stage.An 80 mmol/L NaCl concentration was suitable for discriminating tolerant and non-tolerant okra genotypes.The pooled ranking of the genotypes,based on individual rankings for each trait(root and shoot length,germination percentage,and relative Na+ and K+)in individual NaCl concentrations,was effective for selecting tolerant genotypes.Genotypes selected at the seedling stage maintained their tolerance to NaCl during plant ontogeny,suggesting that screening of the germplasm entries and advanced breeding materials for salt tolerance at the seedling stage is effective.Among 39 okra genotypes,five were identified as the most tolerant genotypes and showed potential for use in breeding programs that focus on the development of salt-tolerant,high-yield okra cultivars.展开更多
文摘The treatment of shavings,trimmings and splits of leather waste from tanneries has a potential to generate value-added products. In this study enzymatic treatment of leather waste was performed. This method utilizes alkaline protease produced by Bacillus subtilis in our laboratory by submerged fermentation. Optimum conditions of pH,time duration,temperature and concentration of enzyme were determined for maximum degradation of leather waste. The amount of degradation was measured by the release of amino acid hydroxyproline. Amino acid composition in the hydrolysate obtained by the enzyme hydrolysis was determined. This relative simple biotreatment of leather waste may provide a practical and economical solution.
文摘The present study is concerned with the isolation and screening of different strains of Aspergillus oryzae for the production of alpha amylase. Ninety strains were isolated from soil and tested for the production of alpha amylase in shake flasks. Of all the strains tested, Aspergillus oryzae GCB-32 and Aspergillus oryzae GCB-35 gave maximum production of alpha amylase. Different culture media were screened for the production of alpha amylase by these two strains. M1 medium containing starch, yeast extract, NH4Cl, MgSO4·7H2O and CaCl2 gave the maximum production of alpha amylase by both the strains Aspergillus oryzae GCB-32 and Aspergillus oryzae GCB-35.Kinetic analysis revealed that the values of product yield coefficient（Y p/x ） and specific product yield coefficient（qp） were found highly significant（p≤0.05） when medium M1 was used for the enzyme production.
基金Project supported by the Indigenous 5000 Fellowship Program(Batch II)of the Higher Education Commission,Pakistan
文摘The okra germplasm was screened for salinity tolerance at the seedling stage and during plant ontogeny.Substantial variation existed in okra for salinity tolerance at the seedling stage.An 80 mmol/L NaCl concentration was suitable for discriminating tolerant and non-tolerant okra genotypes.The pooled ranking of the genotypes,based on individual rankings for each trait(root and shoot length,germination percentage,and relative Na+ and K+)in individual NaCl concentrations,was effective for selecting tolerant genotypes.Genotypes selected at the seedling stage maintained their tolerance to NaCl during plant ontogeny,suggesting that screening of the germplasm entries and advanced breeding materials for salt tolerance at the seedling stage is effective.Among 39 okra genotypes,five were identified as the most tolerant genotypes and showed potential for use in breeding programs that focus on the development of salt-tolerant,high-yield okra cultivars.
