乙型肝炎病毒(hepatitis B virus,HBV)是一种小环型部分双链DNA病毒,属于嗜肝病毒属。HBV没有直接的肝细胞毒性,其感染后宿主免疫系统与病毒之间复杂的相互作用引发的免疫性肝损伤是其致病的重要机制。天然免疫是机体抵抗病原微生物入...乙型肝炎病毒(hepatitis B virus,HBV)是一种小环型部分双链DNA病毒,属于嗜肝病毒属。HBV没有直接的肝细胞毒性,其感染后宿主免疫系统与病毒之间复杂的相互作用引发的免疫性肝损伤是其致病的重要机制。天然免疫是机体抵抗病原微生物入侵的第一道防线,病原体相关分子模式(pathogen associated molecular patterns,PAMPs)被病原体识别受体(pathogens recognition receptors,PRRs)识别并启动天然免疫应答。肝细胞上表达的PRRs介导的天然免疫应答在HBV感染相关的免疫效应中发挥重要作用。HBV在与宿主免疫系统的博弈过程中已经进化出逃避天然免疫识别的主动策略,或主动干扰天然免疫信号通路诱导免疫抑制,从而有利于自身复制。充分理解肝细胞介导的天然免疫应答在参与控制HBV感染过程中的作用及其调控机制,有助于设计新的免疫治疗策略来预防并根除病毒。展开更多
Montmorillonite (MMT) was modified through intercalative polymerization of phenol and formaldehyde catalyzed by oxalic acid. The modified montmorillonite was delaminated at large, as demonstrated by XRD and TEM studi...Montmorillonite (MMT) was modified through intercalative polymerization of phenol and formaldehyde catalyzed by oxalic acid. The modified montmorillonite was delaminated at large, as demonstrated by XRD and TEM studies. It can disperse easily in epoxy resin to form exfoliated nanocomposites. The nanoscale silicate platelets dispersed in water can be metallized by silver deposition.展开更多
Objective： Detecting the expression and mutation of human telomeric repeat binding factor （hTRF1） in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudoge...Objective： Detecting the expression and mutation of human telomeric repeat binding factor （hTRF1） in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify ifhTRF1 mutation is one of the factors of the activation of telomerase. Methods： hTRFlcDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of h TRFlmRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-I, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification （TRAP）-ELISA protocol. PCR and sequencing were used to detect mutation of each exon ofhTRF1 in 10 cell line cells. Results： hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes ofhTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRFI-13, ψhTRFI-18, ψhTRF1-21 and ψhTRFI-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells （0.0338, 0.0108-0.0749） than in normal mononuclear cells （0.0493, 0.0369-0.128） （P=0.004）. But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part ofintron 1, 2 and 8 ofhTRF1. Their infection on gene function is unknown and needs further studies. Conclusion： hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease.展开更多
A facile glycosylation for the synthesis of spirostanol glycosides was reported. Using cadmium carbonate as a catalyst in CH3CN, a series of saponins was synthesized efficiently in a satisfactory yield.
The primer, AT For1, AT For2 and AT Back, are designed and synthesized for adding-PCR that is used to construct the fusion GST-AT gene. Depending on two-time adding-PCR amplification and the insertion of coding sequen...The primer, AT For1, AT For2 and AT Back, are designed and synthesized for adding-PCR that is used to construct the fusion GST-AT gene. Depending on two-time adding-PCR amplification and the insertion of coding sequence of octapeptide carbon skeleton into the 3 terminus of GST gene, the authors get the masculine recon, pGAT-1, confirmed by sequence determination and analysis, whose ORF is read-through. After IPTG induction and partial purification, SDS-PAGE electrophoresis is employed to detect the gene expression. The expressions of total bacterial proteins are about 21.3%, 22.5%, 19.32%, 21.73% in E. coli BL21, DH5α, JM109 and Top10 strains, respectively. Considering the quantity of induced total bacteria, the E. coli BL21 is the best one among the four recipient strains. This article supplies an academic foundation for producing biological active amatoxins.展开更多
Coordinating all the activities among all the parties involved in supply chain can be a daunting task. This paper put forth the viewpoint of applying agent technology to automate the coordination and decision-making t...Coordinating all the activities among all the parties involved in supply chain can be a daunting task. This paper put forth the viewpoint of applying agent technology to automate the coordination and decision-making tasks in a typical home PC industry supply chain. The main features of the proposed approach, which differentiate it from other approaches, are the following:① In the prototype, the coordination agents have both cooperation and competition patterns; ② It uses JADE (Java Agent DEvelopment Framework) as the agent development environment to realize efficient and reusable agent software;③ It produces some innovative models for the business processes and issues faced by parties in the supply chain. A prototype and the overall process flow were also described.展开更多
The three types of connections (Permanent Connection, Soft Permanent Connection and Switched Connection) provided by ASON can adapt the requirement of different network services. Management and maintenance of these th...The three types of connections (Permanent Connection, Soft Permanent Connection and Switched Connection) provided by ASON can adapt the requirement of different network services. Management and maintenance of these three connections are the most important aspect of ASON management. The information models proposed in this paper are used for the purpose of ASON connection management. Firstly a new information model is proposed to meet the requirement for the control plane introduced by ASON. In this model, a new class ControlNE is given, and the relationship between the ControlNE and the transport NE (network element) is also defined. Then this paper proposes information models for the three types of connections for the first time, and analyzes the relationship between the three kinds of connections and the basic network transport entities. Finally, the paper defines some CORBA interfaces for the management of the three connections. In these interfaces, some operations such as create or release a connection are defined, and some other operations can manage the performance of the three kinds of connections, which is necessary for a distributed management system.展开更多
This paper presents a 3 D.O.F haptic interface which is designed to meet the interaction requirement of teleoperation tasks and virtual reality applications. The mechanism design takes the operability into considerati...This paper presents a 3 D.O.F haptic interface which is designed to meet the interaction requirement of teleoperation tasks and virtual reality applications. The mechanism design takes the operability into consideration such as adopting steel cable as transmission component and mass balance to eliminate the gravity effect. The dynamics of haptic interface including actuating device is studied. In order to provide operator with fidelity kinesthetic information, a force controller using self-learning fuzzy logic control is designed. The simulation results verify the effectiveness of the control method.展开更多
A PUMA562 robot has been connected to the Internet and can be controlled through the web browser. The remote user receives still images of robot workspace, coordinates and gripper state and can control the gripper to ...A PUMA562 robot has been connected to the Internet and can be controlled through the web browser. The remote user receives still images of robot workspace, coordinates and gripper state and can control the gripper to pick up blocks and place them to build a structure. An Internet server and a robot server were built to implement this telerobot system. This two-layer server architecture can be used in other Internet-based telerobot system without changing most part of the system.展开更多
Since the plague natural focus of Jianchuan was found in 1974, it had broken the disputation whether Yunnan has plague natural focus among Chinese and foreign scholars for long. Because the focus locates the middle pa...Since the plague natural focus of Jianchuan was found in 1974, it had broken the disputation whether Yunnan has plague natural focus among Chinese and foreign scholars for long. Because the focus locates the middle part of transversal mountains with higher biological diversity and complex landscape, many problems raised at beginning in distinguishing the major hosts and vectors, modeling the structure of the focus, and resolving the contradiction between theory and excitation. In review of that, according to the principle of unified of biological structure and function, the basic plague pattern has been systematically studied on through the generalized information concept in this paper. It suggests that the focus takes the community of Apodemus chevrieri + Rattus norvegicus: Neopsylla specialis + Frontopsylla. spadix + Menopsyllus anisus + Loptopsylla segnis as maintenance subsystem, the community of E. miletus: Ctenophthalmus quadratus + Neopsylla specialis as epidematic (amplifying) subsystem, the communities of squirrel rodent-flea as alternate subsystem. The relationship between subsystems is nonlinear. No human plague case is determined by the systematicness of the plague ecosystem. The possibility of human plague will remain in systemic changing or coming into chaos. Although most researches try to analysis of plague as system by means of experiments with many quantitative criterion, these measures are difficult to comprehension the systemic essence without application of the concept of systemic theory. They are often direct, discursive and paradoxical description of appearance rather than the analysis and generalization of interaction relationship between elements, thus reversing the basic ecological concept of Y. pestis as a living thing and agent of plague.展开更多
文摘Montmorillonite (MMT) was modified through intercalative polymerization of phenol and formaldehyde catalyzed by oxalic acid. The modified montmorillonite was delaminated at large, as demonstrated by XRD and TEM studies. It can disperse easily in epoxy resin to form exfoliated nanocomposites. The nanoscale silicate platelets dispersed in water can be metallized by silver deposition.
文摘Objective： Detecting the expression and mutation of human telomeric repeat binding factor （hTRF1） in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify ifhTRF1 mutation is one of the factors of the activation of telomerase. Methods： hTRFlcDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of h TRFlmRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-I, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification （TRAP）-ELISA protocol. PCR and sequencing were used to detect mutation of each exon ofhTRF1 in 10 cell line cells. Results： hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes ofhTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRFI-13, ψhTRFI-18, ψhTRF1-21 and ψhTRFI-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells （0.0338, 0.0108-0.0749） than in normal mononuclear cells （0.0493, 0.0369-0.128） （P=0.004）. But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part ofintron 1, 2 and 8 ofhTRF1. Their infection on gene function is unknown and needs further studies. Conclusion： hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease.
文摘A facile glycosylation for the synthesis of spirostanol glycosides was reported. Using cadmium carbonate as a catalyst in CH3CN, a series of saponins was synthesized efficiently in a satisfactory yield.
文摘The primer, AT For1, AT For2 and AT Back, are designed and synthesized for adding-PCR that is used to construct the fusion GST-AT gene. Depending on two-time adding-PCR amplification and the insertion of coding sequence of octapeptide carbon skeleton into the 3 terminus of GST gene, the authors get the masculine recon, pGAT-1, confirmed by sequence determination and analysis, whose ORF is read-through. After IPTG induction and partial purification, SDS-PAGE electrophoresis is employed to detect the gene expression. The expressions of total bacterial proteins are about 21.3%, 22.5%, 19.32%, 21.73% in E. coli BL21, DH5α, JM109 and Top10 strains, respectively. Considering the quantity of induced total bacteria, the E. coli BL21 is the best one among the four recipient strains. This article supplies an academic foundation for producing biological active amatoxins.
基金Qingmiao Foundation of Antai School ofManagement of SJTU
文摘Coordinating all the activities among all the parties involved in supply chain can be a daunting task. This paper put forth the viewpoint of applying agent technology to automate the coordination and decision-making tasks in a typical home PC industry supply chain. The main features of the proposed approach, which differentiate it from other approaches, are the following:① In the prototype, the coordination agents have both cooperation and competition patterns; ② It uses JADE (Java Agent DEvelopment Framework) as the agent development environment to realize efficient and reusable agent software;③ It produces some innovative models for the business processes and issues faced by parties in the supply chain. A prototype and the overall process flow were also described.
文摘The three types of connections (Permanent Connection, Soft Permanent Connection and Switched Connection) provided by ASON can adapt the requirement of different network services. Management and maintenance of these three connections are the most important aspect of ASON management. The information models proposed in this paper are used for the purpose of ASON connection management. Firstly a new information model is proposed to meet the requirement for the control plane introduced by ASON. In this model, a new class ControlNE is given, and the relationship between the ControlNE and the transport NE (network element) is also defined. Then this paper proposes information models for the three types of connections for the first time, and analyzes the relationship between the three kinds of connections and the basic network transport entities. Finally, the paper defines some CORBA interfaces for the management of the three connections. In these interfaces, some operations such as create or release a connection are defined, and some other operations can manage the performance of the three kinds of connections, which is necessary for a distributed management system.
文摘This paper presents a 3 D.O.F haptic interface which is designed to meet the interaction requirement of teleoperation tasks and virtual reality applications. The mechanism design takes the operability into consideration such as adopting steel cable as transmission component and mass balance to eliminate the gravity effect. The dynamics of haptic interface including actuating device is studied. In order to provide operator with fidelity kinesthetic information, a force controller using self-learning fuzzy logic control is designed. The simulation results verify the effectiveness of the control method.
文摘A PUMA562 robot has been connected to the Internet and can be controlled through the web browser. The remote user receives still images of robot workspace, coordinates and gripper state and can control the gripper to pick up blocks and place them to build a structure. An Internet server and a robot server were built to implement this telerobot system. This two-layer server architecture can be used in other Internet-based telerobot system without changing most part of the system.
文摘Since the plague natural focus of Jianchuan was found in 1974, it had broken the disputation whether Yunnan has plague natural focus among Chinese and foreign scholars for long. Because the focus locates the middle part of transversal mountains with higher biological diversity and complex landscape, many problems raised at beginning in distinguishing the major hosts and vectors, modeling the structure of the focus, and resolving the contradiction between theory and excitation. In review of that, according to the principle of unified of biological structure and function, the basic plague pattern has been systematically studied on through the generalized information concept in this paper. It suggests that the focus takes the community of Apodemus chevrieri + Rattus norvegicus: Neopsylla specialis + Frontopsylla. spadix + Menopsyllus anisus + Loptopsylla segnis as maintenance subsystem, the community of E. miletus: Ctenophthalmus quadratus + Neopsylla specialis as epidematic (amplifying) subsystem, the communities of squirrel rodent-flea as alternate subsystem. The relationship between subsystems is nonlinear. No human plague case is determined by the systematicness of the plague ecosystem. The possibility of human plague will remain in systemic changing or coming into chaos. Although most researches try to analysis of plague as system by means of experiments with many quantitative criterion, these measures are difficult to comprehension the systemic essence without application of the concept of systemic theory. They are often direct, discursive and paradoxical description of appearance rather than the analysis and generalization of interaction relationship between elements, thus reversing the basic ecological concept of Y. pestis as a living thing and agent of plague.
