Two new ionone derivatives, named rhododendrone and rhododendronside, were isolated from the alcoholic extract of the aerial parts of Rhododendron przwalskii Maxim. Their structures were elucidated on the basis of spe...Two new ionone derivatives, named rhododendrone and rhododendronside, were isolated from the alcoholic extract of the aerial parts of Rhododendron przwalskii Maxim. Their structures were elucidated on the basis of spectroscopic analysis展开更多
The configurations of substituted phenyl furoisoxazolines were determined by study on their 1H-NMR and 1D NOESY. The characteristic chemical shifts of the g-lactone ring protons in 1H-NMR were also discussed.
A novel alkaloid was isolated from the stems and leaves of Stemona parviflora Wright. Based on the spectral methods, its structure was elucidated as parvineostemonine.
A large human naive single chain antibody (scFv) library is constructed from 60 healthy donors via phage display technique. During the period, some methods are employed to optimize the diversity, such as multi donors,...A large human naive single chain antibody (scFv) library is constructed from 60 healthy donors via phage display technique. During the period, some methods are employed to optimize the diversity, such as multi donors, different annealing temperature, half nest PCR, and assembly by two way fusion PCR. In this study, 78 electroporations resulted in 1010 library, diversity of which is assayed by enzyme fingerprint. The efficiency and diversity are all better than other researches.展开更多
文摘Two new ionone derivatives, named rhododendrone and rhododendronside, were isolated from the alcoholic extract of the aerial parts of Rhododendron przwalskii Maxim. Their structures were elucidated on the basis of spectroscopic analysis
文摘The configurations of substituted phenyl furoisoxazolines were determined by study on their 1H-NMR and 1D NOESY. The characteristic chemical shifts of the g-lactone ring protons in 1H-NMR were also discussed.
基金supported by the National Natural Science Foundation of China (No. 30123005) and Bicoll Biotechnology Shanghai Co Ltd. Authors thank Professor Qiong Xin ZHONG (Department of Biological Science Hainan Normal University) for collecting Stemona parviflora
文摘A novel alkaloid was isolated from the stems and leaves of Stemona parviflora Wright. Based on the spectral methods, its structure was elucidated as parvineostemonine.
文摘A large human naive single chain antibody (scFv) library is constructed from 60 healthy donors via phage display technique. During the period, some methods are employed to optimize the diversity, such as multi donors, different annealing temperature, half nest PCR, and assembly by two way fusion PCR. In this study, 78 electroporations resulted in 1010 library, diversity of which is assayed by enzyme fingerprint. The efficiency and diversity are all better than other researches.
