Traditional Chinese medicine(TCM) has played a positive role in the management of hepatocarcinoma. Hepatocarcinoma patients may present Qi-stagnation, damp-heat, blood stasis, Qi-deficiency, Yin-deficiency and other T...Traditional Chinese medicine(TCM) has played a positive role in the management of hepatocarcinoma. Hepatocarcinoma patients may present Qi-stagnation, damp-heat, blood stasis, Qi-deficiency, Yin-deficiency and other TCM syndromes(Zheng). Modern treatments such as surgery, transarterial chemoembolization(TACE) and high intensity focus ultrasound treatment would influence the manifestation of TCM syndromes. Herbs with traditional efficacy of tonifying Qi, blood and Yin, soothing liver-Qi stagnation, clearing heat and detoxifying and dissolving stasis, have been demonstrated to be potent to prevent hepatocarcinogenesis. TCM has been widely used in all aspects of integrative therapy in hepatocarcinoma, including surgical resection, liver transplantation, TACE, local ablative therapies and even as monotherapy for middle-advanced stage hepatocarcinoma. Clinical practices have confirmed that TCM is effective to alleviate clinical symptoms, improve quality of life and immune function, prevent recurrence and metastasis, delay tumor progression, and prolong survival time in hepatocarcinoma patients. The effective mechanism of TCM against hepatocarcinoma is related to inducing apoptosis, autophagy, anoikis and cell senescence, arresting cell cycle, regulating immune function, inhibiting metastasis and angiogenesis, reversing drug resistance and enhancing effects of chemotherapy. Along with the progress of research in this field, TCM will contribute more to the prevention and treatment of hepatocarcinoma.展开更多
AIM To study the role of microbial metabolites in the modulation of biochemical and physiological processes in irritable bowel syndrome(IBS).METHODS In the current study, using a metabolomic approach, we analyzed the ...AIM To study the role of microbial metabolites in the modulation of biochemical and physiological processes in irritable bowel syndrome(IBS).METHODS In the current study, using a metabolomic approach, we analyzed the key metabolites differentially excreted in the feces of control mice and mice with IBS, with or without Clostridium butyricum(C. butyricum) treatment. C57 BL/6 mice were divided into control, IBS, and IBS + C. butyricum groups. In the IBS and IBS + C. butyricum groups, the mice were subjected to water avoidance stress(WAS) for 1 h/d for ten days. Gas chromatography/mass spectrometry(GC-MS) together with multivariate analysis was employed to compare the fecal samples between groups. RESULTS WAS exposure established an appropriate model of IBS in mice, with symptoms of visceral hyperalgesia and diarrhea. The differences in the metabolite profiles between the control group and IBS group significantly changed with the progression of IBS(days 0, 5, 10, and 17). A total of 14 differentially excreted metabolites were identified between the control and IBS groups, and phenylethylamine was a major metabolite induced by stress. In addition, phenylalanine metabolism was found to be the most relevant metabolic pathway. Between the IBS group and IBS + C. butyricum group, 10 differentially excreted metabolites were identified. Among these, pantothenate and coenzyme A(Co A) biosynthesis metabolites, as well as steroid hormone biosynthesis metabolites were identified as significantly relevant metabolic pathways.CONCLUSION The metabolic profile of IBS mice is significantly altered compared to control mice. Supplementation with C. butyricum to IBS mice may provide a considerable benefit by modulating host metabolism.展开更多
BACKGROUND Dyspepsia is one of the commonest clinical disorder.However,controversy remains over the role of endoscopy in patients with dyspepsia.No studies have evaluated the diagnostic value of endoscopy in patients ...BACKGROUND Dyspepsia is one of the commonest clinical disorder.However,controversy remains over the role of endoscopy in patients with dyspepsia.No studies have evaluated the diagnostic value of endoscopy in patients with no warning symptoms according to the Rome IV criteria.AIM To study the diagnostic value of endoscopy in dyspeptic patients with no warning symptoms.METHODS This cross-sectional study included dyspeptic patients with no warning symptoms who met the inclusion and exclusion criteria at The First Affiliated Hospital,Zhejiang Chinese Medical University from April 2018 to February 2019.The clinical data were collected using questionnaires,including dyspeptic information,warning symptoms,other diseases,family history and basic demographic data.Based on dyspeptic symptoms,patients can be divided into epigastric pain syndrome,postprandial distress syndrome or overlapping subtypes.RESULTS A total of 1016 cases were enrolled,304(29.9%)had clinically significant findings that were detectable by endoscopy.The endoscopy findings included esophageal lesions in 180(17.7%)cases,peptic ulcers in 115(11.3%)cases and malignancy in 9(0.89%)patients.Multivariate logistic regression analysis showed that males[odds ratio(OR)=1.758,P<0.001],body mass index>25(OR=1.660;P=0.005),epigastric pain(OR=1.423;P=0.019)and Helicobacter pylori infection(OR=1.949;P<0.001)were independently associated with risk factors for the presence of clinically significant findings on endoscopy.CONCLUSION Chinese patients with dyspepsia with no warning symptoms should undergo endoscopy,particularly males,patients with body mass index>25,epigastric pain or Helicobacter pylori infection.展开更多
BACKGROUND The incidence of infection with Mycobacterium abscessus(M.abscessus)has increased in recent years.This increase is partly associated with invasive cosmetic procedures.CASE SUMMARY The purpose of this case s...BACKGROUND The incidence of infection with Mycobacterium abscessus(M.abscessus)has increased in recent years.This increase is partly associated with invasive cosmetic procedures.CASE SUMMARY The purpose of this case summary is to increase clinicians'awareness of M.abscessus infection and reduce mycobacterial infection caused by cosmetic procedures.We report the case of a 45-year-old woman who received acetyl hexapeptide-8(argireline)injections in the forehead and temples,and erythema,nodules,and abscesses appeared at the injection sites after one week.The pus specimens were examined by microbiological culture and confirmed to be positive for M.abscessus.Clarithromycin 500 mg twice daily and moxifloxacin 400 mg once daily were administered for 5 mo and the lesions gradually subsided.CONCLUSION We report here for the first time a case of infection with M.abscessus after argireline injection.This condition is easily misdiagnosed as a common bacterial infection.Microbiological examinations are helpful for diagnosis and standardized cosmetic procedures can prevent infection with M.abscessus.展开更多
Constructing controllable thermal conduction networks is the key to efficiently improve thermal conductivities of polymer composites.In this work,graphite oxide(GO)and functionalized carbon nanotubes(f-CNTs)are combin...Constructing controllable thermal conduction networks is the key to efficiently improve thermal conductivities of polymer composites.In this work,graphite oxide(GO)and functionalized carbon nanotubes(f-CNTs)are combined to prepare“Line-Plane”-like hetero-structured thermally conductive GO@f-CNTs fillers,which are then performed to construct controllable 3D GO@f-CNTs thermal conduction networks via selfsacrificing template method based on oxalic acid.Subsequently,thermally conductive GO@f-CNTs/polydimethylsiloxane(PDMS)composites are fabricated via casting method.When the size of oxalic acid is 0.24 mm and the volume fraction of GO@f-CNTs is 60 vol%,GO@f-CNTs/PDMS composites present the optimal thermal conductivity coefficient(λ,4.00 W·m^(-1)·K^(-1)),about 20 times that of theλof neat PDMS(0.20 W·m^(-1)·K^(-1)),also much higher than theλ(2.44 W·m^(-1)·K^(-1))of GO/f-CNTs/PDMS composites with the same amount of randomly dispersed fillers.Meanwhile,the obtained GO@f-CNTs/PDMS composites have excellent thermal stability,whoseλdeviation is only about 3%after 500 thermal cycles(20-200℃).展开更多
Herein, functionalized graphene oxide（GO） was prepared by the covalent functionalization with amino acids（lysine, glycine, glutamic acid and tyrosine） in this study. Zeta potential results demonstrated that covale...Herein, functionalized graphene oxide（GO） was prepared by the covalent functionalization with amino acids（lysine, glycine, glutamic acid and tyrosine） in this study. Zeta potential results demonstrated that covalent functionalization of GO with amino acids was favourable for their homogeneous dispersion in water and organic solvents. Based on the higher absolute value of zeta potential and the better dipersion stability of GO-lysine, the PVDF/GO-lysine hybrid membranes were then prepared via the phase inversion induced by immersion precipitation technique. SEM images showed a better pore diameter and porosity distribution on the PVDF/GO-lysine membrane surface. The zeta potential absolute value of the PVDF/GO-lysine membrane surface was higher than that of the virgin PVDF membrane. Furthermore, the PVDF/GO-lysine membranes surface exhibited good hydrophilicity. The water flux of PVDF/GO-lysine membranes can reach two times of that of the virgin PVDF membrane. And the BSA adsorbed amount on PVDF/GO-lysine surface was decreased to 0.82 mg/cm^2 for PVDF/GO-lysine-8% membrane. Filtration experiment results indicated that the fouling resistance was significantly improved for the PVDF/GO-lysine membranes. As a result, lysine functionalized GO will provide a promising method to fabricate graphene oxide based hybrid membranes with effective antifouling property and hydrophilicity.展开更多
To the Editor:Major depressive disorder is one of the most leading causes of disability worldwide.The molecular pathophysiology of depression is complicated and has already attracted intensive and considerable attenti...To the Editor:Major depressive disorder is one of the most leading causes of disability worldwide.The molecular pathophysiology of depression is complicated and has already attracted intensive and considerable attentionj11 Nowadays,the neurotrophic hypothesis of depression suggests that brain-derived neurotrophic factor(BDNF)/tyrosine kinase receptor B(TrkB)receptor binding activates the downstream signaling pathways to culminate in cell survival.This process is essential for neurogenesis and synaptogenesis,which are involved in the effects of antidepressants.展开更多
Background: Teng-Long-Bu-Zhong-Tang(TLBZT) is a traditional Chinese herbal medicine used to treat colorectal cancer(CRC). In the present study, we observed the anti-cancer effects of TLBZT on human RKO CRC. Materials ...Background: Teng-Long-Bu-Zhong-Tang(TLBZT) is a traditional Chinese herbal medicine used to treat colorectal cancer(CRC). In the present study, we observed the anti-cancer effects of TLBZT on human RKO CRC. Materials and Methods: Mice were subcutaneously transplanted with RKO cells, divided into control, 5-Fu-administered, TLBZT-administered, and TLBZT and 5-Fu combination-administered groups, and treated with 5-Fluorouracil(5-Fu) and/or TLBZT. Apoptosis was detected by Td T-mediated d UTP nick-end labeling assay. The activity of caspase-3,-8, and-9 was detected using specific commercial kits. Cell senescence was assessed using senescence β-galactosidase staining. Protein expression was evaluated by immunohistochemistry. Results: TLBZT inhibited RKO CRC tumor growth, enhanced the anti-cancer effects of 5-Fu, induced apoptosis, and activated caspase-3,-8, and-9. TLBZT induced cell senescence accompanied by the downregulation of cyclin E1 and cyclin-dependent kinase 2 expressions. TLBZT also inhibited angiogenesis and the expression of hypoxia-inducible factor 1 subunit alpha and vascular endothelial growth factor-A. Conclusions: TLBZT inhibited RKO CRC tumor growth and enhanced the anti-cancer effects of 5-Fu, and it could be associated with apoptosis and cell senescence induction, and angiogenesis inhibition.展开更多
基金National Natural Science Foundation of China,Nos.81273726 and 81473625Three-year Action Program of Shanghai Municipality for Traditional Chinese Medicine,No.ZY3-CCCX-3-3025
文摘Traditional Chinese medicine(TCM) has played a positive role in the management of hepatocarcinoma. Hepatocarcinoma patients may present Qi-stagnation, damp-heat, blood stasis, Qi-deficiency, Yin-deficiency and other TCM syndromes(Zheng). Modern treatments such as surgery, transarterial chemoembolization(TACE) and high intensity focus ultrasound treatment would influence the manifestation of TCM syndromes. Herbs with traditional efficacy of tonifying Qi, blood and Yin, soothing liver-Qi stagnation, clearing heat and detoxifying and dissolving stasis, have been demonstrated to be potent to prevent hepatocarcinogenesis. TCM has been widely used in all aspects of integrative therapy in hepatocarcinoma, including surgical resection, liver transplantation, TACE, local ablative therapies and even as monotherapy for middle-advanced stage hepatocarcinoma. Clinical practices have confirmed that TCM is effective to alleviate clinical symptoms, improve quality of life and immune function, prevent recurrence and metastasis, delay tumor progression, and prolong survival time in hepatocarcinoma patients. The effective mechanism of TCM against hepatocarcinoma is related to inducing apoptosis, autophagy, anoikis and cell senescence, arresting cell cycle, regulating immune function, inhibiting metastasis and angiogenesis, reversing drug resistance and enhancing effects of chemotherapy. Along with the progress of research in this field, TCM will contribute more to the prevention and treatment of hepatocarcinoma.
基金Supported by the National Natural Science Foundation of China,No.81470814 and No.81400594Zhejiang Provincial Natural Science Foundation of China,No.LQ14H160014
文摘AIM To study the role of microbial metabolites in the modulation of biochemical and physiological processes in irritable bowel syndrome(IBS).METHODS In the current study, using a metabolomic approach, we analyzed the key metabolites differentially excreted in the feces of control mice and mice with IBS, with or without Clostridium butyricum(C. butyricum) treatment. C57 BL/6 mice were divided into control, IBS, and IBS + C. butyricum groups. In the IBS and IBS + C. butyricum groups, the mice were subjected to water avoidance stress(WAS) for 1 h/d for ten days. Gas chromatography/mass spectrometry(GC-MS) together with multivariate analysis was employed to compare the fecal samples between groups. RESULTS WAS exposure established an appropriate model of IBS in mice, with symptoms of visceral hyperalgesia and diarrhea. The differences in the metabolite profiles between the control group and IBS group significantly changed with the progression of IBS(days 0, 5, 10, and 17). A total of 14 differentially excreted metabolites were identified between the control and IBS groups, and phenylethylamine was a major metabolite induced by stress. In addition, phenylalanine metabolism was found to be the most relevant metabolic pathway. Between the IBS group and IBS + C. butyricum group, 10 differentially excreted metabolites were identified. Among these, pantothenate and coenzyme A(Co A) biosynthesis metabolites, as well as steroid hormone biosynthesis metabolites were identified as significantly relevant metabolic pathways.CONCLUSION The metabolic profile of IBS mice is significantly altered compared to control mice. Supplementation with C. butyricum to IBS mice may provide a considerable benefit by modulating host metabolism.
基金the National Natural Science Foundation of China,No.81770535。
文摘BACKGROUND Dyspepsia is one of the commonest clinical disorder.However,controversy remains over the role of endoscopy in patients with dyspepsia.No studies have evaluated the diagnostic value of endoscopy in patients with no warning symptoms according to the Rome IV criteria.AIM To study the diagnostic value of endoscopy in dyspeptic patients with no warning symptoms.METHODS This cross-sectional study included dyspeptic patients with no warning symptoms who met the inclusion and exclusion criteria at The First Affiliated Hospital,Zhejiang Chinese Medical University from April 2018 to February 2019.The clinical data were collected using questionnaires,including dyspeptic information,warning symptoms,other diseases,family history and basic demographic data.Based on dyspeptic symptoms,patients can be divided into epigastric pain syndrome,postprandial distress syndrome or overlapping subtypes.RESULTS A total of 1016 cases were enrolled,304(29.9%)had clinically significant findings that were detectable by endoscopy.The endoscopy findings included esophageal lesions in 180(17.7%)cases,peptic ulcers in 115(11.3%)cases and malignancy in 9(0.89%)patients.Multivariate logistic regression analysis showed that males[odds ratio(OR)=1.758,P<0.001],body mass index>25(OR=1.660;P=0.005),epigastric pain(OR=1.423;P=0.019)and Helicobacter pylori infection(OR=1.949;P<0.001)were independently associated with risk factors for the presence of clinically significant findings on endoscopy.CONCLUSION Chinese patients with dyspepsia with no warning symptoms should undergo endoscopy,particularly males,patients with body mass index>25,epigastric pain or Helicobacter pylori infection.
基金The Scientific Research Project of Peking University Shenzhen Hospital,No.JCYJ2018011the San-ming Project of Medicine in Shenzhen,No.SZSM201812059.
文摘BACKGROUND The incidence of infection with Mycobacterium abscessus(M.abscessus)has increased in recent years.This increase is partly associated with invasive cosmetic procedures.CASE SUMMARY The purpose of this case summary is to increase clinicians'awareness of M.abscessus infection and reduce mycobacterial infection caused by cosmetic procedures.We report the case of a 45-year-old woman who received acetyl hexapeptide-8(argireline)injections in the forehead and temples,and erythema,nodules,and abscesses appeared at the injection sites after one week.The pus specimens were examined by microbiological culture and confirmed to be positive for M.abscessus.Clarithromycin 500 mg twice daily and moxifloxacin 400 mg once daily were administered for 5 mo and the lesions gradually subsided.CONCLUSION We report here for the first time a case of infection with M.abscessus after argireline injection.This condition is easily misdiagnosed as a common bacterial infection.Microbiological examinations are helpful for diagnosis and standardized cosmetic procedures can prevent infection with M.abscessus.
基金financially supported by the National Natural Science Foundation of China(No.51973173)Technological Base Scientific Research Projects(Highly Thermally Conductive Nonmetal Materials)+3 种基金Natural Science Foundation of Chongqing,China(No.2023NSCQ-MSX2547)Shaanxi Province Key Research and Development Plan Project(No.2023-YBGY-461)Fundamental Research Funds for the Central Universities,the Innovation Capability Support Program of Shaanxi(No.2024RS-CXTD-57)financially supported by Polymer Electromagnetic Functional Materials Innovation Team of Shaanxi Sanqin Scholars。
文摘Constructing controllable thermal conduction networks is the key to efficiently improve thermal conductivities of polymer composites.In this work,graphite oxide(GO)and functionalized carbon nanotubes(f-CNTs)are combined to prepare“Line-Plane”-like hetero-structured thermally conductive GO@f-CNTs fillers,which are then performed to construct controllable 3D GO@f-CNTs thermal conduction networks via selfsacrificing template method based on oxalic acid.Subsequently,thermally conductive GO@f-CNTs/polydimethylsiloxane(PDMS)composites are fabricated via casting method.When the size of oxalic acid is 0.24 mm and the volume fraction of GO@f-CNTs is 60 vol%,GO@f-CNTs/PDMS composites present the optimal thermal conductivity coefficient(λ,4.00 W·m^(-1)·K^(-1)),about 20 times that of theλof neat PDMS(0.20 W·m^(-1)·K^(-1)),also much higher than theλ(2.44 W·m^(-1)·K^(-1))of GO/f-CNTs/PDMS composites with the same amount of randomly dispersed fillers.Meanwhile,the obtained GO@f-CNTs/PDMS composites have excellent thermal stability,whoseλdeviation is only about 3%after 500 thermal cycles(20-200℃).
基金financially supported by the National Natural Science Foundation of China(No.51303028)Test Center of Fuzhou University
文摘Herein, functionalized graphene oxide（GO） was prepared by the covalent functionalization with amino acids（lysine, glycine, glutamic acid and tyrosine） in this study. Zeta potential results demonstrated that covalent functionalization of GO with amino acids was favourable for their homogeneous dispersion in water and organic solvents. Based on the higher absolute value of zeta potential and the better dipersion stability of GO-lysine, the PVDF/GO-lysine hybrid membranes were then prepared via the phase inversion induced by immersion precipitation technique. SEM images showed a better pore diameter and porosity distribution on the PVDF/GO-lysine membrane surface. The zeta potential absolute value of the PVDF/GO-lysine membrane surface was higher than that of the virgin PVDF membrane. Furthermore, the PVDF/GO-lysine membranes surface exhibited good hydrophilicity. The water flux of PVDF/GO-lysine membranes can reach two times of that of the virgin PVDF membrane. And the BSA adsorbed amount on PVDF/GO-lysine surface was decreased to 0.82 mg/cm^2 for PVDF/GO-lysine-8% membrane. Filtration experiment results indicated that the fouling resistance was significantly improved for the PVDF/GO-lysine membranes. As a result, lysine functionalized GO will provide a promising method to fabricate graphene oxide based hybrid membranes with effective antifouling property and hydrophilicity.
基金grants from the Education Department of Fujian Province[No.2019-WJ-38]Xiamen Municipal Health Commission[No.2019-WJ-38]Huaqiao University[No.ZQN-PY218].
文摘To the Editor:Major depressive disorder is one of the most leading causes of disability worldwide.The molecular pathophysiology of depression is complicated and has already attracted intensive and considerable attentionj11 Nowadays,the neurotrophic hypothesis of depression suggests that brain-derived neurotrophic factor(BDNF)/tyrosine kinase receptor B(TrkB)receptor binding activates the downstream signaling pathways to culminate in cell survival.This process is essential for neurogenesis and synaptogenesis,which are involved in the effects of antidepressants.
基金supported by the National Natural Science Foundation of China (grant number 81273726)Natural Science Foundation of Shanghai Municipality (grant number 20ZR1458700)the Science and Technology Commission of Shanghai Municipality (grant number 19401933400)。
文摘Background: Teng-Long-Bu-Zhong-Tang(TLBZT) is a traditional Chinese herbal medicine used to treat colorectal cancer(CRC). In the present study, we observed the anti-cancer effects of TLBZT on human RKO CRC. Materials and Methods: Mice were subcutaneously transplanted with RKO cells, divided into control, 5-Fu-administered, TLBZT-administered, and TLBZT and 5-Fu combination-administered groups, and treated with 5-Fluorouracil(5-Fu) and/or TLBZT. Apoptosis was detected by Td T-mediated d UTP nick-end labeling assay. The activity of caspase-3,-8, and-9 was detected using specific commercial kits. Cell senescence was assessed using senescence β-galactosidase staining. Protein expression was evaluated by immunohistochemistry. Results: TLBZT inhibited RKO CRC tumor growth, enhanced the anti-cancer effects of 5-Fu, induced apoptosis, and activated caspase-3,-8, and-9. TLBZT induced cell senescence accompanied by the downregulation of cyclin E1 and cyclin-dependent kinase 2 expressions. TLBZT also inhibited angiogenesis and the expression of hypoxia-inducible factor 1 subunit alpha and vascular endothelial growth factor-A. Conclusions: TLBZT inhibited RKO CRC tumor growth and enhanced the anti-cancer effects of 5-Fu, and it could be associated with apoptosis and cell senescence induction, and angiogenesis inhibition.
