Aim: To study the mitochondrial function damage of sperm induced by reactive oxygen species (ROS) and the protection of melatonin (MLT) against the damage. Methods: Normal function spermatozoa were selected from semen...Aim: To study the mitochondrial function damage of sperm induced by reactive oxygen species (ROS) and the protection of melatonin (MLT) against the damage. Methods: Normal function spermatozoa were selected from semen samples by Percoll gradient centrifugation technique. The ROS generated by the hypoxan-thine xanthine oxidase system was incubated with the normal spermatozoa in the presence or absence of MLT (6 mmol/L) for 30 and 60 minutes. After incubation, the activity of succinate dehydroge-nase (SDH) in the mitochondria of spermatozoa was assessed by histochemical method and spermatozoa were labeled with specific fluorescent probe of Rhodamine 123 to measure the mitochondrial membrane potential (MMP) by flow cytometry. Results: After the normal spermatozoa were incubated with ROS, The sperm MMP was significantly decreased and the SDH activity of almost decreased to zero. MLT reduced the mitochondrial damage induced by ROS. Conclusion: ROS damage the mitochondrial function of sperm by affecting sperm MMP and SDH activity of. MLT protects sperm mitochondria from the damage induced by ROS through its effective antioxidative potential.展开更多
Objective: To determine the concentration of malondialdehyde (MDA), product of lipid peroxidation, in seminal plasma and evalu ate its significance. Methods: Ninety-three cases of infertile pa tients were divided into...Objective: To determine the concentration of malondialdehyde (MDA), product of lipid peroxidation, in seminal plasma and evalu ate its significance. Methods: Ninety-three cases of infertile pa tients were divided into the obstructive azoospermic (12 cases), the non-obstructive azoospermic (15 cases), the oligozoospermic (21 cases), the asthenozoospermic (19 cases), the oligoastheno zoospermic (16 cases) and the oligoasthenoteratozoospermic (10) groups. Eighteen fertile males served as the controls. MDA con centration was assessed by high-performance liquid chromatogra phy (HPLC). Results: With the exception of the obstructive azoospermic group, the MDA concentration in the seminal plasma was significantly different between the control group and the infer tile groups (P<0.01) as well as between the different infertile groups. Conclusion: Determination of MDA concentration in the seminal plasma is helpful to the diagnosis of male infertility induced by overproduction of reactive oxygen species.展开更多
When Yang Jiang was celebrating her lo3rd birthday, People＇sLiterature Publishing House published the Complete Works of Yang Jiang（Nine Volumes）, collecting all of the renowned author, translator and scholar Yang J...When Yang Jiang was celebrating her lo3rd birthday, People＇sLiterature Publishing House published the Complete Works of Yang Jiang（Nine Volumes）, collecting all of the renowned author, translator and scholar Yang Jiang＇s works including essays and novels. Her works like novel Baptism.展开更多
Recent advances in OCR show that end-to-end(E2E)training pipelines including detection and identification can achieve the best results.However,many existing methods usually focus on case insensitive English characters...Recent advances in OCR show that end-to-end(E2E)training pipelines including detection and identification can achieve the best results.However,many existing methods usually focus on case insensitive English characters.In this paper,we apply an E2E approach,the multiplex multilingual mask TextSpotter,which performs script recognition at the word level and uses different recognition headers to process different scripts while maintaining uniform loss,thus optimizing script recognition and multiple recognition headers simultaneously.Experiments show that this method is superior to the single-head model with similar number of parameters in endto-end identification tasks.展开更多
文摘Aim: To study the mitochondrial function damage of sperm induced by reactive oxygen species (ROS) and the protection of melatonin (MLT) against the damage. Methods: Normal function spermatozoa were selected from semen samples by Percoll gradient centrifugation technique. The ROS generated by the hypoxan-thine xanthine oxidase system was incubated with the normal spermatozoa in the presence or absence of MLT (6 mmol/L) for 30 and 60 minutes. After incubation, the activity of succinate dehydroge-nase (SDH) in the mitochondria of spermatozoa was assessed by histochemical method and spermatozoa were labeled with specific fluorescent probe of Rhodamine 123 to measure the mitochondrial membrane potential (MMP) by flow cytometry. Results: After the normal spermatozoa were incubated with ROS, The sperm MMP was significantly decreased and the SDH activity of almost decreased to zero. MLT reduced the mitochondrial damage induced by ROS. Conclusion: ROS damage the mitochondrial function of sperm by affecting sperm MMP and SDH activity of. MLT protects sperm mitochondria from the damage induced by ROS through its effective antioxidative potential.
文摘Objective: To determine the concentration of malondialdehyde (MDA), product of lipid peroxidation, in seminal plasma and evalu ate its significance. Methods: Ninety-three cases of infertile pa tients were divided into the obstructive azoospermic (12 cases), the non-obstructive azoospermic (15 cases), the oligozoospermic (21 cases), the asthenozoospermic (19 cases), the oligoastheno zoospermic (16 cases) and the oligoasthenoteratozoospermic (10) groups. Eighteen fertile males served as the controls. MDA con centration was assessed by high-performance liquid chromatogra phy (HPLC). Results: With the exception of the obstructive azoospermic group, the MDA concentration in the seminal plasma was significantly different between the control group and the infer tile groups (P<0.01) as well as between the different infertile groups. Conclusion: Determination of MDA concentration in the seminal plasma is helpful to the diagnosis of male infertility induced by overproduction of reactive oxygen species.
文摘When Yang Jiang was celebrating her lo3rd birthday, People＇sLiterature Publishing House published the Complete Works of Yang Jiang（Nine Volumes）, collecting all of the renowned author, translator and scholar Yang Jiang＇s works including essays and novels. Her works like novel Baptism.
基金supported by the Advanced Training Project of the Professional Leaders in Jiangsu Higher Vocational Colleges (2020GRFX006).
文摘Recent advances in OCR show that end-to-end(E2E)training pipelines including detection and identification can achieve the best results.However,many existing methods usually focus on case insensitive English characters.In this paper,we apply an E2E approach,the multiplex multilingual mask TextSpotter,which performs script recognition at the word level and uses different recognition headers to process different scripts while maintaining uniform loss,thus optimizing script recognition and multiple recognition headers simultaneously.Experiments show that this method is superior to the single-head model with similar number of parameters in endto-end identification tasks.
