Retraction note to:RNA-binding protein CPSF6 regulates IBSP to affect pyroptosis in gastric cancer

The authors have decided to retract the article published on World J Gastrointest Oncol(2023)for further consideration due to data errors and some misunderstandings in communication.

RNA-binding protein CPSF6 regulates IBSP to affect pyroptosis in gastric cancer

BACKGROUND Extensive evidence has illustrated the promotive role of integrin binding sialoprotein(IBSP)in the progression of multiple cancers.However,little is known about the functions of IBSP in gastric cancer(GC)progression.AIM To investigate the mechanism underlying the regulatory effects of IBSP in GC progression,and the relationship between IBSP and cleavage and polyadenylation factor 6(CPSF6)in this process.METHODS The mRNA and protein expression of relevant genes were assessed through realtime quantitative polymerase chain reaction and Western blot,respectively.Cell viability was evaluated by Cell Counting Kit-8 assay.Cell invasion and migration were evaluated by Transwell assay.Pyroptosis was measured by flow cytometry.The binding between CPSF6 and IBSP was confirmed by luciferase reporter and RNA immunoprecipitation(RIP)assays.RESULTS IBSP exhibited higher expression in GC tissues and cell lines than in normal tissues and cell lines.IBSP knockdown suppressed cell proliferation,migration,and invasion but facilitated pyroptosis.In the exploration of the regulatory mechanism of IBSP,potential RNA binding proteins for IBSP were screened with catRAPID omics v2.0.The RNA-binding protein CPSF6 was selected due to its higher expression in stomach adenocarcinoma.Luciferase reporter and RIP assays revealed that CPSF6 binds to the 3’-untranslated region of IBSP and regulates its expression.Knockdown of CPSF6 inhibited cell proliferation,migration,and invasion but boosted pyroptosis.Through rescue assays,it was uncovered that the retarded GC progression mediated by CPSF6 knockdown was reversed by IBSP overexpression.CONCLUSION Our study highlighted the vital role of the CPSF6/IBSP axis in GC,suggesting that IBSP might be an effective biotarget for GC treatment.

MicroRNA-486和TGF-β_1对人主动脉瓣膜钙化的影响及其机制研究

目的研究microRNA-486(miRNA-486)对人主动脉瓣膜间质细胞(VICs)钙化的影响及作用机制。方法体外培养VICs,经成骨诱导后用双荧光素报告基因检测miRNA-486靶基因。转染miR-486mimic、miR-486 Inhibitors后,将细胞分为miR-486 mimic组、miR-486 mimic NC组、miR-486 Inhibitors组、miR-486 Inhibitors NC组。采用茜红素染色、ALP试验分析各组钙化程度,实时荧光定量聚合酶链反应(qRT-PCR)、免疫荧光试验检测miR-486靶基因及成骨相关因子mRNA和蛋白的表达水平。结果双荧光素报告基因检测和Western blot检测结果显示,转录生长因子-β_1(TGF-β_1)为潜在靶基因。茜红素染色、ALP试验结果显示,miR-486 mimic组钙化程度高于miR-486 Inhibitors组。qRT-PCR、免疫荧光试验结果表明,miR-486 mimic组TGF-β_1、SMAD3 mRNA和蛋白表达水平较低(P<0.05);miR-486 mimic组RUNX2、骨钙素mRNA表达水平较miR-486 Inhibitors组高(P<0.05)。结论 miR-486可通过下调TGF-β_1的表达,促进RUNX2、骨钙素的表达和活性,诱导间质细胞向成骨细胞分化。

Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model

AIM To investigate gut microbial diversity and the interventional effect of Xiaoyaosan(XYS) in a rat model of functional dyspepsia(FD) with liver depression-spleen deficiency syndrome. METHODS The FD with liver depression-spleen deficiency syndrome rat model was established through classic chronic mild unpredictable stimulation every day. XYS group rats received XYS 1 h before the stimulation. The models were assessed by parameters including state ofthe rat, weight, sucrose test result and open-field test result. After 3 wk, the stools of rats were collected and genomic DNA was extracted. PCR products of the V4 region of 16 S rD NA were sequenced using a barcoded Illumina paired-end sequencing technique. The primary composition of the microbiome in the stool samples was determined and analyzed by cluster analysis.RESULTS Rat models were successfully established, per data from rat state, weight and open-field test. The microbiomes contained 20 phyla from all samples. Firmicutes, Bacteroidetes, Proteobacteria, Cyanobacteria and Tenericutes were the most abundant taxonomic groups. The relative abundance of Firmicutes, Proteobacteria and Cyanobacteria in the model group was higher than that in the normal group. On the contrary, the relative abundance of Bacteroidetes in the model group was lower than that in the normal group. Upon XYS treatment, the relative abundance of all dysregulated phyla was restored to levels similar to those observed in the normal group. Abundance clustering heat map of phyla corroborated the taxonomic distribution. CONCLUSION The microbiome relative abundance of FD rats with liver depression-spleen deficiency syndrome was significantly different from the normal cohort. XYS intervention may effectively adjust the gut dysbacteriosis in FD.

Validation of the chinese version of the EORTC QLQ-CR29 in patients with colorectal cancer

AIM To assess the validity and reliability of the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-Colorectal Cancer 29(EORTC QLQ-CR29) in Chinese patients with colorectal cancer(CRC). METHODS From March 2014 to January 2015, 356 patients with CRC from four different hospitals in China were enrolled in the study, and all patients self-administered the EORTC QLQ-CR29 and the quality of life core questionnaire(EORTC QLQ-C30). Evaluation of the scores was based on the Karnofsky Performance Scale(KPS). The reliability and validity of the questionnaires were assessed by Cronbach's α coefficient, the Spearman correlation test and Wilcoxon rank sum test.RESULTS The EORTC QLQ-CR29 showed satisfactory reliability(α > 0.7), although the urinary frequency and blood and mucus in stool dimensions had only moderate reliability(α = 0.608). The multitrait scaling analyses showed good convergent(r > 0.4) and discriminant validity. Significant differences were obtained for each item in the different KPS subgroups(KPS ≤ 80; KPS > 80). body image and most single-item dimensions showed statistically significant differences in patients with a stoma compared with the rest of the patients. CONCLUSION The EORTC QLQ-CR29 exhibits high validity and reliability in Chinese patients with CRC, and can therefore be recommended as a valuable tool for the assessment of quality of life in these patients.

Chemoprophylaxis, diagnosis, treatments, and discharge management of COVID-19: An evidence-based clinical practice guideline(updated version)

The novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the cause of a rapidly spreading illness,coronavirus disease 2019(COVID-19),affecting more than seventeen million people around the world.Diagnosis and treatment guidelines for clinicians caring for patients are needed.In the early stage,we have issued"A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus(2019-nCoV)infected pneumonia(standard version)";now there are many direct evidences emerged and may change some of previous recommendations and it is ripe for develop an evidence-based guideline.We formed a working group of clinical experts and methodologists.The steering group members proposed 29 questions that are relevant to the management of COVID-19 covering the following areas:chemoprophylaxis,diagnosis,treatments,and discharge management.We searched the literature for direct evidence on the management of COVID-19,and assessed its certainty generated recommendations using the Grading of Recommendations,Assessment,Development and Evaluation(GRADE)approach.Recommendations were either strong or weak,or in the form of ungraded consensus-based statement.Finally,we issued 34 statements.Among them,6 were strong recommendations for,14 were weak recommendations for,3 were weak recommendations against and 11 were ungraded consensus-based statement.They covered topics of chemoprophylaxis(including agents and Traditional Chinese Medicine(TCM)agents),diagnosis(including clinical manifestations,reverse transcription-polymerase chain reaction(RT-PCR),respiratory tract specimens,IgM and IgG antibody tests,chest computed tomography,chest X-ray,and CT features of asymptomatic infections),treatments(including lopinavirritonavir,umifenovir,favipiravir,interferon,remdesivir,combination of antiviral drugs,hydroxychloroquine/chloroquine,interleukin-6 inhibitors,interleukin-1 inhibitors,glucocorticoid,qingfei paidu decoction,lianhua qingwen granules/capsules,convalescent plasma,lung transplantation,invasive or noninvasive ventilation,and extracorporeal membrane oxygenation(ECMO)),and discharge management(including discharge criteria and management plan in patients whose RT-PCR retesting shows SARS-CoV-2 positive after discharge).We also created two figures of these recommendations for the implementation purpose.We hope these recommendations can help support healthcare workers caring for COVID-19 patients.

Expression of S100B during the innate immune of corneal epithelium against fungi invasion

AIM： To explore the expression of SIOOB in corneal epithelial cells under ,Aspergillus stimulation both in vivo and in vitro. METHODS： Immortalized human corneal epithelial cells （HCECs） were exposed to inactive #lsperg///us fumigatus （A. fumigatus） conidia at 0, 4, 8, 12, 16, and 24h respectively. The corneas of Wistar rats were exposed to active A. fumigatus at 0, 12, 24, 48h and the normal rat corneas were used for normal control. The mRNA level of S100B was evaluated by real time quantitative reverse transcription-polymerase chain reaction （qRT-PCR）. Sl00B protein expression in cornea epithelium was detected by immunohistochemical/immunocytochemical staining （IHC/ICC）. RESULTS： Histopathology revealed a significant inflammatory cell infiltration in fungal keratitis human and rat cornea. Corneal epithelial cells didn＇t express or rarely express S100B at baseline. A. fumigatus significantly induced S100B mRNA expression in cultured corneal epithelial cells in a time depended manner in vitro the mRNA began to rise significantly at 8h in vitro （P〈0.05） and continue to rise as time prolonged （P〈0.01）. in vivo S100B mRNA level was low in the normal corneas. However, it was increased in keratitis corneas from 12h after infection （P〈0.05） and reached to a peak at 24h （P〈0.001）. Immunochemistry revealed an obvious staining in fungal keratitis corneas as well as immortalized HCECs compared to the normal ones respectively, indicating an increased expression of SlOOB protein. CONCLUSION： S100B exists in corneal epithelial cells and is over-expressed under A. fumigatus stimulation. Sl00B may play an important role in the innate immune response of the corneal epithelium during A. fumigatus infection.

新型冠状病毒肺炎药物预防、诊断、治疗与出院管理临床指南计划书

本计划书为《新型冠状病毒(2019-nCoV)感染的肺炎诊疗快速建议指南(标准版)》更新版的研究方案。计划书涵盖指南制定方法学、指南的目标用户、指南的目标人群、利益冲突调查与声明、临床问题和结局指标的遴选与确定、证据的检索、合成与评价、证据与推荐意见分级标准、推荐意见的形成、指南的更新,并附录指南临床问题清单、文献检索策略及利益冲突调查表。

Notoginsenoside R1 attenuates breast cancer progression by targeting CCND2 and YBX3

Background:Breast cancer(BC)is a common malignancy with highly female incidence.So far the function of notoginsenoside R1(NGR1),the extract from Panax notoginseng,has not been clearly elucidated in BC.Methods:Optimal culture concentration and time of NGR1 were investigated by cell counting kit-8 assay.Cell proliferation ability was measured by colony formation assays.Transwell assay was used to detect the effect of NGR1 on cell migration and invasion.The apoptosis rate of cells between each group was measured by TUNEL assay.Results:NGR1 treatment has an inhibitory efect on proliferation,migration,invasion,and angiogenesis and a stimulating effect on cell cycle arrest and apoptosis of Michigan Cancer Foundation-7(MCF-7)cells.The 50%growth inhibitory concentration for MCF-7 cells at 24 h was 148.9 mmol/L.The proportions of MCF-7 cells arrested in the G0/G1 phase were 36.94+6.78%,45.06士5.60%,and 59.46+5.60%in the control group,75,and 150 mmol/L groups,respectively.Furthermore,we revealed that NGR1 treatment attenuates BC progression by targeted downregulating CCND2 and YBX3 genes.Additionally,YBX3 activates phosphatidylinositol 3-phosphate kinase(PI3K)/protein kinase B(Akt)signaling pathway by activating kirsten rat sarcoma viral oncogene,which is an activator of the PI3K/Akt signaling pathway.Conclusion:These results suggest that NGR1 can act as an efficacious drug candidate that targets the YBX3/PI3K/Akt axis in patients with BC.

Hajek-Rényi-type Inequality and Strong Law of Large Numbers for Some Dependent Sequences

Abstract In this paper, we get the H^jek-R^nyi-type inequalities for a pairwise NQD sequence, an L^T （r 〉 1） mixingale and a linear process, which have the concrete coefficients. In addition, we obtain the strong law of large numbers, strong growth rate and the integrability of supremum for the above sequences, which generalize and improve Corollary 2 for L^T（r 〉 1） mixingale of Hansen.

The Finite-time Ruin Probability of a Discrete-time Risk Model with Subexponential and Dependent Insurance and Financial Risks

Consider a discrete-time risk model with insurance and financial risks in a stochastic economic environment. Assume that the insurance and financial risks form a sequence of independent and identically distributed random vectors with a generic random vector following a wide type of dependence structure. An asymptotic formula for the finite-time ruin probability with subexponential insurance risks is derived. In doing so, the subexponentiality of the product of two dependent random variables is investigated simultaneously.

The Bahadur Representation for Sample Quantiles Under Dependent Sequence

On the one hand,we investigate the Bahadur representation for sample quantiles underφ-mixing sequence withφ(n)=O(n^-3)and obtain a rate as O(n-3/4 log n),a.s.On the other hand,by relaxing the condition of mixing coefficients to∑∞n=1φ^1/2(n)<∞,a rate O(n^-1/2(log n)^1/2),a.s.,is also obtained.

The Consistency for the Estimators of Semiparametric Regression Model with Dependent Samples

For the semiparametric regression model:Y^((j))(x_(in),t_(in))=t_(in)β+g(x_(in))+e^((j))(x_(in)),1≤j≤k,1≤i≤n,where t_(in)∈R and x(in)∈Rpare known to be nonrandom,g is an unknown continuous function on a compact set A in R^(p),e^(j)(x_(in))are m-extended negatively dependent random errors with mean zero,Y^((j))(x_(in),t_(in))represent the j-th response variables which are observable at points xin,tin.In this paper,we study the strong consistency,complete consistency and r-th(r>1)mean consistency for the estimatorsβ_(k,n)and g__(k,n)ofβand g,respectively.The results obtained in this paper markedly improve and extend the corresponding ones for independent random variables,negatively associated random variables and other mixing random variables.Moreover,we carry out a numerical simulation for our main results.

Some Exponential Inequalities for Negatively Ort han t Dependent Random Variables

In the paper,we establish some exponential inequalities for non-identically distributed negatively orthant dependent(NOD,for short)random variables.In addition,we also establish some exponential inequalities for the partial sum and the maximal partial sum of identically distributed NOD random variables.As an application,the Kolmogorov strong law of large numbers for identically distributed NOD random variables is obtained.Our results partially generalize or improve some known results.

Pharmacological and pharmacodynamic evaluation of original and generic calcipotriol ointments

Objective This study is aimed to investigate the differences in pharmaceutical quality and therapeutic efficacy of calcipotriol ointments between the original product and the generics.Methods We evaluated the pharmaceutical quality of the original product and the generics including pH,content and related substance,and calcipotriol stability in the matrix.Intradermal retention of calcipotriol ointments in vitro was investigated using skin of Bama miniature pig.The pharmacodynamics study was performed in psoriasis guinea pig models.Results The matrix of original product is better than the generics,and influenced the drug diffusion,stability and in vitro retention,thus resulted in the difference in pharmacodynamics.The retention amount of calcipotriol in the original product was significantly higher than that of generic B,and approximately 4 folds higher than that of generic A.All of the three ointments improved pathological indexed in psoriasis guinea pigs,and there was a significant difference between the preparation group and the model group (P =0.001).Guinea pigs in the original group had the lowest interleukin-17 level (35.7 ± 1.13 μg/ml) and Baker Score (2.70 ± 0.97) compared with generic A (37.9 ±3.3 μg/ml,3.10 ± 0.22,respectively) and generic B (39 ± 4.6μg/ml,3.80 ± 0.57,respectively),but no statistical difference was found among the three groups (all P ＞ 0.05).Conclusions Difference occurs in the internal quality between the original product and the generics.The original preparation owns suitable balance between matrix stability and transdermal ability so that showes superiority in pharmaceutical properties,intradermal retention and therapeutic effect in vitro and in vivo,which suggests that more attention should be paid to the formulation and process during the research and development of the generics.The original product is recommended with priority for treatment especially at emergency.