高通量测序文库质量控制技术研究进展

作为生命科学和医学领域中的关键性支撑技术,高通量测序已得到快速发展并日趋成熟。该技术工作流程可分为核酸提取、文库构建、上机测序、数据分析等,其中文库构建是承上启下的关键步骤,文库构建的效果受制于上游样品质量,同时会对测序数据产出后的数据分析造成影响。对文库构建质量控制技术的选择和实施是提高结果可靠性、降低测序数据误差的重要保证。本文对文库构建质量控制技术进行深入综述,总结评价其原理、优缺点、适用范围,并对实际应用场景中相关技术的选择进行了论述,以期为科研人员、疾病预防控制人员等在选择文库质量控制技术时提供理论依据与参考,从而促进高通量测序工作的质量和效率。

HI in High Gas-phase Metallicity Dwarf Galaxy WISEA J230615.06+143927.9

We present resolved Giant Metrewave Radio Telescope H I observations of the high gas-phase metallicity dwarf galaxy WISEA J230615.06+143927.9(z = 0.005)(hereafter J2306) and investigate whether it could be a Tidal Dwarf Galaxy(TDG) candidate. TDGs are observed to have higher metallicities than normal dwarfs. J2306 has an unusual combination of a blue g-r color of 0.23 mag, irregular optical morphology and high-metallicity(12 +log(O/H) = 8.68 ± 0.14), making it an interesting galaxy to study in more detail. We find J2306 to be an H I rich galaxy with a large extended, unperturbed rotating H I disk. Using our H I data we estimated its dynamical mass and found the galaxy to be dark matter(DM) dominated within its H I radius. The quantity of DM, inferred from its dynamical mass, appears to rule out J2306 as an evolved TDG. A wide area environment search reveals J2306 to be isolated from any larger galaxies which could have been the source of its high gas metallicity. Additionally, the H I morphology and kinematics of the galaxy show no indication of a recent merger to explain the high-metallicity.Further detailed optical spectroscopic observations of J2306 might provide an answer to how a seemingly ordinary irregular dwarf galaxy achieved such a high level of metal enrichment.

强电用超导材料的发展现状与展望

超导材料具有常规材料不具备的零电阻、完全抗磁性等宏观量子现象,是典型的量子材料。在强电应用领域,使用超导材料可以实现常规技术无法实现的超强磁场、大容量储能等诸多颠覆性技术,因此,强电用超导材料制备技术一直是国际高技术竞争前沿。本文通过梳理国内外强电用超导材料及其制备技术的发展现状,系统分析和阐明了包括低温超导材料NbTi、Nb_(3)Sn和高温超导材料YBCO涂层导体、Bi-2223带材、Bi-2212线材以及MgB_(2)线材等实用化超导材料在强电应用领域的发展趋势。分析我国强电用超导材料发展存在的问题,我国需要以开发出面向不同强电应用需求的高性能超导材料体系为基础,实现超导材料和强电应用产品的协同发展,推动强电用超导材料制备技术和应用技术的创新水平提升和产业化规模。研究建议,通过国家层面组织“产学研用”联合攻关,实现低温超导材料产业升级,突破高温超导材料批量化制备关键技术的发展思路,实现强电用超导材料的快速发展和应用。

Application Analysis of Doctor-Nurse Cooperation Follow-up Model Based on the Internet Platform in Continuation Care of Patients after Urooncology Surgery

Objective:To explore the application effect of a doctor-nurse cooperation follow-up model based on an Internet platform in the continuation of care for patients after uro-oncology surgery.Methods:A convenient sampling method was used to select patients with urinary system tumors who underwent surgery in the Department of Urology in Grade III A general hospital in Shanghai from May to August 2022.Patients who underwent surgery from May to June 2022 were assigned to the control group,and those who underwent surgery from July to August 2022 were assigned to the experimental group.The control group received routine post-discharge nursing health education and telephone follow-up.On the basis of routine discharge guidance,the experimental group implemented the intervention method based on the Internet platform in continuation care.The levels of self-management efficacy,satisfaction,and incidence of unplanned readmission were compared one month after discharge between the two groups.Results:One month after discharge,the self-management efficacy of the experimental group(90.15±7.92)was significantly higher than that of the control group(79.10±7.84),and the patient satisfaction score(97.83±2.32)was significantly higher than that of the control group(90.23±2.58),with statistical significance(P<0.05).Additionally,the incidence of unplanned readmissions within one month after discharge in the experimental group(1.59%)was slightly lower than that in the control group(4.84%).Conclusion:The doctor-nurse cooperation follow-up model based on the Internet platform in continuation care can significantly improve the self-management efficiency of patients after discharge and enhance patient satisfaction,providing a new approach for discharge follow-up of urological tumor patients after surgery.

标准化助力国内低温超导线材产业化

2008年6月,西部超导建成了国内首条低温超导线材产业化生产线。经过两年多的工程化试制,于2010年底开始向国际热核聚变实验堆(ITER)项目批量供货,拉开了国内低温超导线材产业化的序幕。近几年来随着我国低温超导线材工艺的不断优化改进,性能和质量水平的持续提高,国内国际市场逐步拓宽,使得低温超导线材产业化得以迅猛发展,特别在2022年,低温超导线材突破了1000吨产量大关,跻身于国际前列。在国内整个低温超导线材产业化的发展过程中,标准化起到了至关重要的作用。本文以《实用超导线的扭距测量Nb-Ti与Nb_(3)Sn复合超导体的扭距测量方法》标准为例,介绍其制定工作和应用情况,以此来阐明标准化对低温超导线材产业发展的助推作用。

GeTe基热电材料的研究进展

GeTe基材料在热电领域有着巨大的发展潜力,已成为近年来中温区热电材料的研究重点。首先简要介绍了GeTe的晶体结构和相变过程;其次,从提升其热电性能方法的角度对该体系近年来的研究进展进行综述:(1)以调控功率因子为目的,对GeTe体系的空穴载流子浓度进行优化,提升Ge的空位能;(2)通过能带工程实现能带结构的调控;(3)以降低热导率为目的,引入纳米缺陷(点缺陷、堆垛缺陷、纳米沉淀),增强声子散射效果等。最后对GeTe基热电材料的研究进展进行了总结,并对其今后的研究发展提出展望。

Photocatalytic and Electrocatalytic Generation of Hydrogen Peroxide: Principles, Catalyst Design and Performance

Hydrogen peroxide(H_(2)O_(2)) is a high-demand organic chemical reagent and has been widely used in various modern industrial applications. Currently,the prominent method for the preparation of H_(2)O_(2) is the anthraquinone oxidation.Unfortunately, it is not conducive to economic and sustainable development since it is a complex process and involves unfriendly environment and potential hazards. In this context, numerous approaches have been developed to synthesize H_(2)O_(2). Among them, photo/electro-catalytic ones are considered as two of the most promising manners for on-site synthesis of H_(2)O_(2). These alternatives are sustainable in that only water or O_(2) is required. Namely, water oxidation(WOR) or oxygen reduction(ORR)reactions can be further coupled with clean and sustainable energy. For photo/electro-catalytic reactions for H_(2)O_(2) generation, the design of the catalysts is extremely important and has been extensively conducted with an aim to obtain ultimate catalytic performance. This article overviews the basic principles of WOR and ORR,followed by the summary of recent progresses and achievements on the design and performance of various photo/electro-catalysts for H_(2)O_(2) generation. The related mechanisms for these approaches are highlighted from theoretical and experimental aspects. Scientific challenges and opportunities of engineering photo/electro-catalysts for H_(2)O_(2) generation are also outlined and discussed.

Inhibiting phosphatase and actin regulator 1 expression is neuroprotective in the context of traumatic brain injury

Studies have found that the phosphatase actin regulatory factor 1 expression can be related to stroke,but it remains unclear whether changes in phosphatase actin regulatory factor 1 expression also play a role in traumatic brain injury.In this study we found that,in a mouse model of traumatic brain injury induced by controlled cortical impact,phosphatase actin regulatory factor 1 expression is increased in endothelial cells,neurons,astrocytes,and microglia.When we overexpressed phosphatase actin regulatory factor 1 by injection an adeno-associated virus vector into the contused area in the traumatic brain injury mice,the water content of the brain tissue increased.However,when phosphatase actin regulatory factor 1 was knocked down,the water content decreased.We also found that inhibiting phosphatase actin regulatory factor 1 expression regulated the nuclear factor kappa B signaling pathway,decreased blood-brain barrier permeability,reduced aquaporin 4 and intercellular adhesion molecule 1 expression,inhibited neuroinflammation,and neuronal apoptosis,thereby improving neurological function.The findings from this study indicate that phosphatase actin regulatory factor 1 may be a potential therapeutic target for traumatic brain injury.

Biosynthesis and Immunological Evaluation of a Dual-Antigen Nanoconjugate Vaccine Against Brucella melitensis

Brucellosis,caused by Brucella,is one of the most common zoonosis.However,there is still no vaccine for human use.Although some live attenuated vaccines have been approved for animals,the protection effect is not ideal.In this study,we developed a dual-antigen nanoconjugate vaccine containing both polysaccharide and protein antigens against Brucella.First,the antigenic polysaccharide was covalently coupled to the outer membrane protein Omp19 using protein glycan coupling technology,and then it was successfully loaded on a nano-carrier through the SpyTag/SpyCatcher system.After confirming the efficient immune activation and safety performance of the dual-antigen nanoconjugate vaccine,the potent serum antibody response against the two antigens and remarkable protective effect in non-lethal and lethal Brucella infection models were further demonstrated through different routes of administration.These results indicated that the dual-antigen nanoconjugate vaccine enhanced both T helper 1 cell(Th1)and Th2 immune responses and protected mice from Brucella infection.Furthermore,we found that this protective effect was maintained for at least 18 weeks.To our knowledge,this is the first Brucella vaccine bearing diverse antigens,including a protein and polysaccharide,on a single nanoparticle.Thus,we also present an attractive technology for co-delivery of different types of antigens using a strategy applicable to other vaccines against infectious diseases.

Gut microbiome:New perspectives for type 2 diabetes prevention and treatment

Type 2 diabetes mellitus(T2DM),which is distinguished by increased glucose levels in the bloodstream,is a metabolic disease with a rapidly increasing incidence worldwide.Nevertheless,the etiology and characteristics of the mechanism of T2DM remain unclear.Recently,abundant evidence has indicated that the intestinal microbiota is crucially involved in the initiation and progression of T2DM.The gut microbiome,the largest microecosystem,engages in material and energy metabolism in the human body.In this review,we concentrated on the correlation between the gut flora and T2DM.Meanwhile,we summarized the pathogenesis involving the intestinal flora in T2DM,as well as therapeutic approaches aimed at modulating the gut microbiota for the management of T2DM.Through the analysis presented here,we draw attention to further exploration of these research directions.

Transcriptome and co-expression network analysis uncover the key genes mediated by endogenous defense hormones in tea plant in response to the infestation of Empoasca onukii Matsuda

The tea green leafhopper[Empoasca(Matsumurasca)onukii Matsuda]is one of the most dominant pests in the tea production regions of China,greatly impacting tea yield and quality.Extensive studies have attributed the plant defense response to pest infestation to the activation of hormone signals,but the role of defense hormones in tea plants in response to the feeding of E.onukii is not clear.RNA-seq was performed on the leaf samples collected at different time points(6,24 and 48 h)after feeding with E.onukii to explore the transcription characteristics of tea plant responses to feeding.The content variation characteristics of four phytohormones(JA,JA-Ile,ABA and SA)during this process were also determined.The results showed that feeding elevated the expression of key genes involved in phenylpropanoid biosynthesis,flavonoid biosynthesis,α-linolenic acid metabolism and terpenoids synthesis pathways,especially in the early stage after feeding.The contents of JA,JA-Ile,and ABA dramatically increased at different time points after feeding,but the absolute content decreased over time.The change in the absolute content of SA was opposite to that of the above three hormones.Two gene co-expression networks that were significantly positively associated with these hormonal changes were constructed based on WGCNA.The screened hub genes provide useful genetic resources for exploring the hormone-mediated defense responses of tea plants against E.onukii.Our results lay the foundation for the ecological control of E.onukii and breeding of resistant tea plant cultivars.

Phellinus igniarius ameliorates renal aging in a rat model of focal and segmental glomerulosclerosis

Objective:To comparatively investigate the ameliorative effect of Phellinus igniarius(P.igniarius)on renal aging in a rat model of focal and segmental glomerulosclerosis(FSGS).Methods:The FSGS model was established in rats by uninephrectomy combined with tail vein injection of doxorubicin.The FSGS rats were randomly divided into the model group,the P.igniarius decoction group,the P.igniarius polysaccharides group,and the P.igniarius polyphenols group.Molecular indicators of cell senescence,renal function indexes,and podocyte injury markers were tested after ten weeks of intragastric administration.Besides,the pathological renal lesions and the ultrastructural changes were observed.Results:FSGS developed in the model group within ten weeks and showed segmental glomerular scarring and renal aging.Following the 10-week intervention,24 h proteinuria,serum creatinine,blood urea nitrogen,P16^(INK4α),thrombospondin-1,and transforming growth factor-β1 were decreased in each treatment group,whereas albumin,erythropoietin,nephrin,and podocin were increased;the pathological renal injury was alleviated,and the number of senescent cells was reduced,especially in rats treated with P.igniarius decoction.Conclusions:P.igniarius ameliorates renal aging and renal injury in the FSGS rat model.Compared with the effective constituents(polysaccharides and polyphenols),P.igniarius decoction has a better curative effect,which is expected to provide a new therapeutic idea for FSGS.

Effect of periodic wide atmospheric pressure change on CO emission in closed goaf

Atmospheric pressure fuctuation is one of the most important factors afecting the climate environment and gas emission in the fre area.To obtain the infuence rule of the surface atmospheric pressure change on the gas sampling and abnormal emission in the mine closed goaf,the No.1 coal mine in Dananhu was taken as the research object.Using Fourier transform and Fisher harmonic analysis and other statistical methods,the infuence of the periodic variation of atmospheric pressure on the gas leakage and outfow in the closed goaf was studied.The results showed that there were three atmospheric pressure periods of 15.2 d,1 d and 182.2 d,and the probability was greater than 95%.The time period with the highest number of atmospheric pressure peaks was 7:00–8:00,which accounted for 20.2%of total occurrence number in a day.And the time periods with the highest number of atmospheric pressure trough were 2:00,15:00 and 16:00,accounting for 27.4%.The peak-to-trough transition time was mainly concentrated around 6 h,and the diurnal variation curve of atmospheric pressure was mainly bimodal.The atmospheric pressure change rate was mostly concentrated in 10–50 Pa/h.It was determined that the distance that the gas sampling pipe was pre-laid into the inner side of the closed wall should be greater than 44.4 m,and the CO concentration and atmospheric pressure in the closed goaf were both periodic and negative with atmospheric pressure.The research results have important guiding signifcance for the monitoring,early warning and environmental protection of the goaf.

Robust beamforming for IRS-aided SWIPT in cognitive radio networks

Intelligent reflecting surface(IRS)is widely recognized as a promising technique to enhance the system perfor-mance,and thus is a hot research topic in future wireless communications.In this context,this paper proposes a robust BF scheme to improve the spectrum and energy harvesting efficiencies for the IRS-aided simultaneous wireless information and power transfer(SWIPT)in a cognitive radio network(CRN).Here,the base station(BS)utilizes spectrum assigned to the primary users(PUs)to simultaneously serve multiple energy receivers(ERs)and information receivers(IRs)through IRS-aided multicast technology.In particular,by assuming that only the imperfect channel state information(CSI)is available,we first formulate a constrained problem to maximize the minimal achievable rate of IRs,while satisfying the harvesting energy threshold of ERs,the quality-of-service requirement of IRs,the interference threshold of PUs and transmit power budget of BS.To address the non-convex problem,we then adopt triangle inequality to deal with the channel uncertainty,and propose a low-complexity algorithm combining alternating direction method of multipliers(ADMM)with alternating optimi-zation(AO)to jointly optimize the active and passive beamformers for the BS and IRS,respectively.Finally,our simulation results confirm the effectiveness of the proposed BF scheme and also provide useful insights into the importance of introducing IRS into the CRN with SWIPT.

Ambient Fast Synthesis of Superaerophobic/Superhydrophilic Electrode for Superior Electrocatalytic Water Oxidation

Developing cost-effective and facile methods to synthesize efficient and stable electrocatalysts for large-scale water splitting is highly desirable but remains a significant challenge.In this study,a facile ambient temperature synthesis of hierarchical nickel-iron(oxy)hydroxides nanosheets on iron foam(FF-FN)with both superhydrophilicity and superaerophobicity is reported.Specifically,the as-fabricated FF-FN electrode demonstrates extraordinary oxygen evolution reaction(OER)activity with an ultralow overpotential of 195 mV at 10 mA cm^(-2)and a small Tafel slope of 34 mV dec^(-1)in alkaline media.Further theoretical investigation indicates that the involved lattice oxygen in nickel-iron-based-oxyhydroxide during electrochemical self-reconstruction can significantly reduce the OER reaction overpotential via the dominated lattice oxygen mechanism.The rechargeable Zn-air battery assembled by directly using the as-prepared FF-FN as cathode displays remarkable cycling performance.It is believed that this work affords an economical approach to steer commercial Fe foam into robust electrocatalysts for sustainable energy conversion and storage systems.

Connected Vehicles Computation Task Offloading Based on Opportunism in Cooperative Edge Computing

The traditional multi-access edge computing (MEC) capacity isoverwhelmed by the increasing demand for vehicles, leading to acute degradationin task offloading performance. There is a tremendous number ofresource-rich and idle mobile connected vehicles (CVs) in the traffic network,and vehicles are created as opportunistic ad-hoc edge clouds to alleviatethe resource limitation of MEC by providing opportunistic computing services.On this basis, a novel scalable system framework is proposed in thispaper for computation task offloading in opportunistic CV-assisted MEC.In this framework, opportunistic ad-hoc edge cloud and fixed edge cloudcooperate to form a novel hybrid cloud. Meanwhile, offloading decision andresource allocation of the user CVs must be ascertained. Furthermore, thejoint offloading decision and resource allocation problem is described asa Mixed Integer Nonlinear Programming (MINLP) problem, which optimizesthe task response latency of user CVs under various constraints. Theoriginal problem is decomposed into two subproblems. First, the Lagrangedual method is used to acquire the best resource allocation with the fixedoffloading decision. Then, the satisfaction-driven method based on trial anderror (TE) learning is adopted to optimize the offloading decision. Finally, acomprehensive series of experiments are conducted to demonstrate that oursuggested scheme is more effective than other comparison schemes.

Effects of acupotomy on the activity of osteoclasts and osteoblasts in the subchondral bone of rabbits with early and mid-stage knee osteoarthritis models

Objective:To investigate whether acupotomy could inhibit subchondral bone remodeling in knee osteoarthritis(KOA)rabbits by regulating the activity of osteoblasts and osteoclasts.Methods:KOA rabbits were prepared by immobilization for 6 and 9 weeks by Videman method.Nine groups of rabbits(control,6 weeks and 9 weeks model,6 weeks and 9 weeks acupotomy,6 weeks and 9 weeks electroacupuncture,and 6 weeks and 9 weeks drug groups)received acupotomy,electroacupuncture and risedronate sodium intervention,respectively,for 3 weeks.Results:Acupotomy can inhibit the activity of osteoclasts and osteoblasts in subchondral bone by reducing the proteins expression of cathepsin K(CK)and tartrate-resistant acid phosphatase(TRAP)and decreasing the proteins expression of osteocalcin(OCN)and alkaline phosphatase(ALP),to intercept the abnormal bone resorption and bone formation of subchondral bone in 6-week and 9-week immobilization-induced KOA rabbits.Conclusion:These findings indicated that acupotomy may be more advantageous than risedronate sodium intervention in modulating subchondral bone remodeling in KOA rabbits,especially in 9-week immobilization-induced KOA rabbits.

Fever induced Brugada electrocardiographic pattern converted to the early repolarization pattern after fever abatement in an elderly man

Brugada syndrome(BrS)is one of inherited arrhythmia syndromes characterized by ST-segment elevation in the right precordial leads and is associated with increased risk of ventricular fibrillation(VF)and sudden cardiac death(SCD)in the absence of a structural heart disease.Fever is a known trigger for Brugada ECG pattern(BrP).[1]Relevant studies showed that BrS patients with a fever induced type 1(F-type 1)BrP have a worse prognosis due to the higher risk of future arrhythmic events.[2]However,the prognosis of asymptomatic subjects with F-type 1 BrP remains to be elucidated,especially in the elderly.Recent studies have found that in elderly patients,F-type 1 BrP led to VF or even syncope.[3,4,5]Thus,more clinical attention should be given rise on cases of elderly asymptomatic patients with F-type 1 BrP.

Intestinal Cckbr-specific knockout mouse as a novel model of salt-sensitive hypertension via sodium over-absorption

OBJECTIVES To investigate the value of CCKBR^(fl/fl)villin-Cre mice as a mouse model of salt-sensitive hypertension(SSH).METHODS In the first part,2-month-old CCKBR^(fl/fl)villin-Cre mice(CKO)and control CCKBR^(fl/fl)mice(WT)were fed with normal diet(0.4%NaCl)or high salt diet(4%NaCl),separately for 6 weeks.In the rescue study,one week of hydrochlorothiazide or saline injection were treated with the CKO mice fed high salt diet.The blood pressure,biochemical indexes,and the expression of small intestinal sodium transporters(NHE3,NKCC1,eNaC)was detected.The organ injury markers(MMP2/MMP9)and the histopathological changes of kidneys were observed,whereas the changes of duodenal sodium absorption were detected by small intestinal perfusion in vivo.RESULTS The CCKBR^(fl/fl)villin-Cre mice with high salt intake exhibited high blood pressure,increased duodenal sodium absorption and urinary sodium excretion,and with renal injury.The protein expression of NHE3,NKCC1 and eNaC were also significant increase in the intestine of CKO-HS mice.Treatment with hydrochlorothiazide remarkably attenuated the elevated blood pressure by high salt absorption in the CCKBR^(fl/fl)villin-Cre mice,but no significant histopathological changes were observed.CONCLUSIONS These results support a crucial role of intestinal Cckbr deficiency on SSH development and the diuretic antihypertension effect in CCKBR^(fl/fl)villin-Cre mice.The CCKBR^(fl/fl)villin-Cre mice with the high salt intake may serve as a stable model of salt-sensitive hypertensive induced by sodium overloading.