Screening,identification and antagonistic effect of antagonistic bacteria JTFM1001 against aflatoxin contamination in corn

Aflatoxin is a strong carcinogenic and toxic fungal toxin produced by Aspergillus flavus and other Aspergillus species,and can seriously threaten the health of consumers,thus becoming a global concern.Corn,as an important oil and economic crop,is highly susceptible contaminated by aflatoxin.In this study,antagonistic bacteria with strong inhibitory effect on aflatoxin were screened to provide support for the treatment aflatoxin contamination control in corn.Ten strains which have strong antagonistic effects against A.flavus were isolated from healthy corn from different corn producing areas in China.Among them,the antagonistic bacteria JTFM1001 through corn kernels in vivo and field experiment,the inhibition effect of aflatoxin contamination reached above 70%and 55%,respectively.And the strain was identified as Bacillus subtilis based on its morphological,physiological and biochemical characteristics and phylogenetic analysis of 16S rDNA.In addition,our data showed that it can colonize in the rhizosphere and survive for a long time,forming the dominant flora,with broad application prospect.Finally,we were surprised to find that the antibacterial metabolites secreted by the antagonistic bacteria was one of the mechanisms of its inhibition of A.flavus and aflatoxin.This will provide us with new ideas and perspectives on the effective prevention and control of aflatoxin contamination in corn and corn oil.

Review on contaminants in edible oil and analytical technologies

Edible oil is an important part of the human daily diet,which is popular in the food industry.People are paying increasing attention to the safety of edible oil as flawed oils have caused enormous international losses and posed a huge threat to consumer health issues.Therefore,it is necessary and important to analyze the quality and safety of oil product.In the present study,we particularly reviewed recent literature on analysis of emerging pollutants in edible oil including mycotoxins,pesticide residues and heavy metals.In addition,a series of emerging analytical technologies(HPLC,LC-MS/MS and GC-MS)for these contaminants were also summarized in this paper.

Influencing Factors and Biological Control of Aflatoxin Contamination in Peanuts

Peanut is an important economic crop and oil crop in China. It is vulnerable to infection by Aspergillus flavus during production,storage,transportation and processing,causing aflatoxin（ AFT） contamination. This paper introduced the status and characteristics of AFT contamination in China,analyzed the infection pathways and influencing factors of A. flavus,and discussed the prevention and control technology of AFT contamination in peanuts from the view of the use of beneficial microorganisms.

Identification of Common Maize Diseases and Their Prevention and Control Methods

Agriculture is the basic industry of China's economic development,and maize is an important part of the agricultural economy.With the increase of global environmental warming and industrial pollution,as well as the adjustment of agricultural planting structure and the transformation of farming and cultivation methods in China,the breeding of maize varieties has been accelerated,and the planting area has increased rapidly,resulting in the condition that the occurrence of maize diseases and pests has been increasing.The damage of some minor diseases continued to increase nationwide or in local areas,and rose to major diseases,and some new diseases appeared in production,posing a threat to the safe production of maize.This paper summarized the occurrence status of important maize diseases in China,and put forward corresponding prevention and control countermeasures.

Single-cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste-modified soils

Application of agricultural waste such as rapeseed meal(RM)is regarded as a sustainable way to improve soil phosphorus(P)availability by direct nutrient supply and stimulation of native phosphate‐solubilizing microorganisms(PSMs)in soils.However,exploration of the in situ microbial P solubilizing function in soils remains a challenge.Here,by applying both phenotype‐based single‐cell Raman with D_(2)O labeling(Raman‐D_(2)O)and genotype‐based high‐throughput chips targeting carbon,nitrogen and P(CNP)functional genes,the effect of RM application on microbial P solubilization in three typical farmland soils was investigated.The abundances of PSMs increased in two alkaline soils after RM application identified by single‐cell Raman D_(2)O.RM application reduced the diversity of bacterial communities and increased the abundance of a few bacteria with reported P solubilization function.Genotypic analysis indicated that RM addition generally increased the relative abundance of CNP functional genes.A correlation analysis of the abundance of active PSMs with the abundance of soil microbes or functional genes was carried out to decipher the linkage between the phenotype and genotype of PSMs.Myxococcota and C degradation genes were found to potentially contribute to the enhanced microbial P release following RM application.This work provides important new insights into the in situ function of soil PSMs.It will lead to better harnessing of agricultural waste to mobilize soil legacy P and mitigate the P crisis.