A procedure for preparing of inositol triphosphate from sodium phytate was established.Hydrolysis was achieved by microwave rediation.When The hydrolysis rate of phytic acid was about 50%,the hydrolyzed solutions were...A procedure for preparing of inositol triphosphate from sodium phytate was established.Hydrolysis was achieved by microwave rediation.When The hydrolysis rate of phytic acid was about 50%,the hydrolyzed solutions were pumped into a column containing 717 strong anion-exchange resin,then delivered a linear gradient of 0.05～0.7MNaCl.the samples of each tube were digested and the phosphate was determined.the contents of tubes corre-sponding to the same individual inositol phosphate were pooled together,and precipitated by Ca2+.The cacium salt was then turned into IP3.Neutralized with sodium bydroxide,rotary evaporated under vaccum,lyophilized,the desired product was gotten.Each inositol phosphate was identified by HPLC and MS.展开更多
文摘A procedure for preparing of inositol triphosphate from sodium phytate was established.Hydrolysis was achieved by microwave rediation.When The hydrolysis rate of phytic acid was about 50%,the hydrolyzed solutions were pumped into a column containing 717 strong anion-exchange resin,then delivered a linear gradient of 0.05～0.7MNaCl.the samples of each tube were digested and the phosphate was determined.the contents of tubes corre-sponding to the same individual inositol phosphate were pooled together,and precipitated by Ca2+.The cacium salt was then turned into IP3.Neutralized with sodium bydroxide,rotary evaporated under vaccum,lyophilized,the desired product was gotten.Each inositol phosphate was identified by HPLC and MS.
