The meso-tetra(α,α,α,α-O-phenylacetylbenzene)porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques. The antibody could combine with Mn porph...The meso-tetra(α,α,α,α-O-phenylacetylbenzene)porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques. The antibody could combine with Mn porphyrin and showed a haloperoxidase activity. The affinities of the antibody for antigen and Mn porphyrin were 4 98×10 -5 mol/L and 7 63×10 -5 mol/L, respectively. When the antibody-Mn porphyrin complex was used to catalyze the halogenation reaction, the Michaelis constant and reactive rate constant κ cat for H 2O 2 were 5 127 0×10 -3 mol/L and 0 483 4 s -1, respectively. The enzyme exchange number κ cat/K m=94 285 1 mol/(L·s).展开更多
采用聚丙烯酰胺垂直电泳技术(PAGE)对来自山东野生、江苏野生、浙江养殖、广西野生白色壳4个文蛤M eretrix m eretrix群体两种组织(消化腺、闭壳肌)的EST、MDH、ME、ADH、SOD、CAT等6种同工酶进行了分析。结果显示:文蛤不同组织之间、...采用聚丙烯酰胺垂直电泳技术(PAGE)对来自山东野生、江苏野生、浙江养殖、广西野生白色壳4个文蛤M eretrix m eretrix群体两种组织(消化腺、闭壳肌)的EST、MDH、ME、ADH、SOD、CAT等6种同工酶进行了分析。结果显示:文蛤不同组织之间、不同群体之间、养殖群体和野生群体之间的同工酶酶谱均有较明显的差别;广西白壳群体的多种同工酶酶谱与其它几个群体相比有较大差异,说明该种群与另外3个群体种质差异较大,亲缘关系较远。对山东野生、广西普通壳色和广西白色壳色3个群体的4种同工酶(EST、AMY、MDH、SOD)进行了研究,结果表明:广西普通壳色群体和山东群体的酶谱相似,而与广西白色壳群体差异明显,初步推测广西白色壳群体为文蛤属的其他种类;在文蛤各群体中均找到了特征性酶带,可以作为区别于文蛤不同群体的蛋白标记,用于文蛤种质资源的分析鉴定,并可为选育优良品种文蛤提供遗传依据。展开更多
文摘The meso-tetra(α,α,α,α-O-phenylacetylbenzene)porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques. The antibody could combine with Mn porphyrin and showed a haloperoxidase activity. The affinities of the antibody for antigen and Mn porphyrin were 4 98×10 -5 mol/L and 7 63×10 -5 mol/L, respectively. When the antibody-Mn porphyrin complex was used to catalyze the halogenation reaction, the Michaelis constant and reactive rate constant κ cat for H 2O 2 were 5 127 0×10 -3 mol/L and 0 483 4 s -1, respectively. The enzyme exchange number κ cat/K m=94 285 1 mol/(L·s).
文摘采用聚丙烯酰胺垂直电泳技术(PAGE)对来自山东野生、江苏野生、浙江养殖、广西野生白色壳4个文蛤M eretrix m eretrix群体两种组织(消化腺、闭壳肌)的EST、MDH、ME、ADH、SOD、CAT等6种同工酶进行了分析。结果显示:文蛤不同组织之间、不同群体之间、养殖群体和野生群体之间的同工酶酶谱均有较明显的差别;广西白壳群体的多种同工酶酶谱与其它几个群体相比有较大差异,说明该种群与另外3个群体种质差异较大,亲缘关系较远。对山东野生、广西普通壳色和广西白色壳色3个群体的4种同工酶(EST、AMY、MDH、SOD)进行了研究,结果表明:广西普通壳色群体和山东群体的酶谱相似,而与广西白色壳群体差异明显,初步推测广西白色壳群体为文蛤属的其他种类;在文蛤各群体中均找到了特征性酶带,可以作为区别于文蛤不同群体的蛋白标记,用于文蛤种质资源的分析鉴定,并可为选育优良品种文蛤提供遗传依据。
