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油茶林土壤放线菌的分离及其拮抗油茶炭疽病菌的筛选 被引量:14
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作者 宋光桃 周国英 +2 位作者 罗秋良 郝艳 何苑皞 《福建林学院学报》 CSCD 北大核心 2009年第4期297-300,共4页
从油茶林不同类型土样中分离到85株放线菌,采用PDA培养基和甲壳素脱乙酰酶培养基进行筛选。结果表明,以油茶炭疽病菌为指示菌,用对峙培养法选出抑菌带宽度达15 mm以上的菌株18株,菌株CF17拮抗效果最明显;CF17对油茶根腐病菌、油茶叶枯... 从油茶林不同类型土样中分离到85株放线菌,采用PDA培养基和甲壳素脱乙酰酶培养基进行筛选。结果表明,以油茶炭疽病菌为指示菌,用对峙培养法选出抑菌带宽度达15 mm以上的菌株18株,菌株CF17拮抗效果最明显;CF17对油茶根腐病菌、油茶叶枯病菌等供试病原真菌也有较好抑菌效果,对各供试病原真菌的抑菌带宽度达14 mm以上;CF18、CF33和CF48菌株能产生甲壳素脱乙酰酶。 展开更多
关键词 土壤 放线菌 分离 拮抗 筛选
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山东省不同植被区内野生植物根围AM菌的生态分布 被引量:28
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作者 盖京苹 刘润进 李晓林 《生态学杂志》 CAS CSCD 北大核心 2000年第4期18-22,共5页
Resources and ecological distribution of Arbuscular mycorrhizal fungi(AMF) on wild plants in Shandong vegetation region were investigated during 1995-1997. A total of 182 rhizospheric soil samples were collected from ... Resources and ecological distribution of Arbuscular mycorrhizal fungi(AMF) on wild plants in Shandong vegetation region were investigated during 1995-1997. A total of 182 rhizospheric soil samples were collected from 50 wild plants. Spores of AMF occurred in all samples. More than fourty species of AMF were isolated and 35 species in five genera were identified. Among the five genera,the frenquency of occurence was Glomus (51.7%)> Scutellospora (23.1)> Gigaspora(14.1%)>Acaulospora(9.5%)>Entrophospora (0.8%). Glomus mosseae, Gigaspora margarita are prominent species, G.versiforme, G.constrictum, S.gilmorei are common species to be isolated. The spore density of AMF was different: Gigaspora>Glomus>Scutellospora>Acaulospora>Entrophospora . The occurence and distribution of AMF are related to regional difference, soil factors(contents of N, P; organic matter and pH) and host plant.The prominent species in eastern part were Gi.margarita, G.mosseae and G.versiforme , while in middle and western part of Shandong province was G.mosseae . The occurence frequency of Glomus, Gigaspora and Scutellospora was much higher than that of other genera in eastern and middle part of Shandong province. While Glomus and Acaulospora were higher in the western part. The spore number of Gigaspora and Scutellospora were much more than that of other genera in the samples collected in the eastern and middle area, while Glomus and Acaulospora are more frequently encountered in the western part of Shandong provence. 展开更多
关键词 山东 植被区 野生植物 根围AM菌 生态分布 发育
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4种内源真菌对马尾松凋落叶分解的影响 被引量:1
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作者 李慧业 林永慧 何兴兵 《西南农业学报》 CSCD 北大核心 2021年第3期618-625,共8页
【目的】从湘西地区马尾松林的凋落叶中分离筛选并鉴定出17种内源真菌,选取4种优势内源真菌,以马尾松凋落叶为分解材料,分析4种内源真菌对马尾松凋落叶分解的影响及微生物活性变化。【方法】将内源真菌接种至凋落叶后用凋落物袋法置于... 【目的】从湘西地区马尾松林的凋落叶中分离筛选并鉴定出17种内源真菌,选取4种优势内源真菌,以马尾松凋落叶为分解材料,分析4种内源真菌对马尾松凋落叶分解的影响及微生物活性变化。【方法】将内源真菌接种至凋落叶后用凋落物袋法置于实验样地1年。【结果】与空白对照组相比内源真菌可以促进凋落物分解。在分解过程中,不同的内源真菌对凋落叶的分解能力存在差异性,混合菌处理组的失重率均高于单菌处理组的失重率且Pestalotiopsis neglecta和Abortiporus biennis混合菌处理组失重率最高;随着时间的增加,微生物CO_2释放量随之减少;不同处理组的微生物活性在不同分解期也存在差异,其中P.neglecta和A.biennis混合菌处理组的纤维素分解酶(C1酶、Cx酶、β-葡萄糖苷酶)和漆酶活性最高,这与失重率的反应一致。【结论】4种马尾松内源真菌定殖在一定程度上促进了后续凋落叶分解以及相应的微生物活性。 展开更多
关键词 马尾松凋落叶 马尾松叶内源真菌 失重率 酶活性
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