Adventitious buds were induced by using tender buds emblic ( Phyllanthus emblica L. ) as explants with MS medium contained different concentration of BA and KT, GA 3,NAA or IAA, of which MS medium combined with 0.5 mg...Adventitious buds were induced by using tender buds emblic ( Phyllanthus emblica L. ) as explants with MS medium contained different concentration of BA and KT, GA 3,NAA or IAA, of which MS medium combined with 0.5 mg·L -1 of BA and 0.1 mg·L -1 of NAA is suitable for adventitious buds inducement, the rate of differentiation reached more than 90%. MS medium with 0.1 mg·L -1 of BA and 0.1 mg·L -1 of NAA is suitable for the growth of shoots, and 2 3 adventitious buds were regenerated in base. But rooting rate reached only about 30% by using 1/2 MS with 0.25 mg·L -1 of NAA or with 0.25 mg·L -1 of NAA and 0.25 mg·L -1 of IBA.展开更多
文摘Adventitious buds were induced by using tender buds emblic ( Phyllanthus emblica L. ) as explants with MS medium contained different concentration of BA and KT, GA 3,NAA or IAA, of which MS medium combined with 0.5 mg·L -1 of BA and 0.1 mg·L -1 of NAA is suitable for adventitious buds inducement, the rate of differentiation reached more than 90%. MS medium with 0.1 mg·L -1 of BA and 0.1 mg·L -1 of NAA is suitable for the growth of shoots, and 2 3 adventitious buds were regenerated in base. But rooting rate reached only about 30% by using 1/2 MS with 0.25 mg·L -1 of NAA or with 0.25 mg·L -1 of NAA and 0.25 mg·L -1 of IBA.
