【目的】探究在不同异质养分环境下,福建柏[Fokienia hodginsii(Dunn)Henry et Thomas]不同家系幼苗叶片光合特性和酶活性差异及变化趋势,为福建柏新品种培育提供理论依据和数据支持。【方法】以福建柏9个1年生家系(454,464,467,474,490...【目的】探究在不同异质养分环境下,福建柏[Fokienia hodginsii(Dunn)Henry et Thomas]不同家系幼苗叶片光合特性和酶活性差异及变化趋势,为福建柏新品种培育提供理论依据和数据支持。【方法】以福建柏9个1年生家系(454,464,467,474,490,493,500,534,541)幼苗为研究对象,采用盆栽试验,设置氮(N)、磷(P)、钾(K)3种不同异质养分环境,以同质养分环境为对照,研究福建柏各家系幼苗叶片的光合特性、荧光参数及酶(超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT))活性和丙二醛(MDA)含量对不同养分斑块的响应差异。在此基础上,计算不同家系福建柏幼苗叶片光合特性、荧光参数及酶活性和MDA含量的表型可塑性指数。【结果】(1)N、P异质斑块下,福建柏幼苗叶片的净光合速率(P_(n))、气孔导度(G_(s))和蒸腾速率(T_(r))均高于同质养分斑块和K异质斑块,493家系在N异质斑块、541家系在P异质斑块中的P_(n)均最高。K异质斑块和同质养分斑块下,福建柏幼苗叶片胞间CO_(2)浓度(C_(i))明显高于N、P异质斑块,N异质斑块下大部分福建柏家系幼苗叶片WUE较高。(2)在N异质斑块中,不同家系福建柏幼苗叶片的初始荧光值(F_(o))和PSⅡ最大光化学量子产量(F_(v)/F_(m))均较高,PSⅡ实际光化学效率(Yield)和光合电子传递速率(ETR)呈极显著正相关,N、P异质斑块中各家系Yield和ETR均较高。各斑块下福建柏家系PSⅡ的潜在活性(F_(v)/F_(o))无明显的变化规律,同一家系的F_(v)/F_(o)峰值在各种斑块间差异较大,其中500家系的F_(v)/F_(o)在各斑块之间差异显著,534家系的F_(v)/F_(o)在各斑块间无显著差异。(3)在P异质斑块中不同家系福建柏幼苗叶片POD活性均最高,在N异质斑块中大部分家系幼苗叶片POD活性较同质养分斑块有一定提升,在K异质斑块下福建柏家系幼苗叶片POD活性较同质养分斑块无明显规律。N、P异质斑块中,各家系福建柏幼苗叶片SOD和CAT活性均高于同质养分斑块,K异质斑块中POD、SOD和CAT 3种酶活性与同质养分斑块差异不大。N、P异质斑块中,福建柏幼苗叶片MDA含量均明显低于同质养分斑块,而K异质斑块中MDA含量则明显高于N、P异质斑块。(4)福建柏家系幼苗叶片荧光参数的表型可塑性指数均值最低,为0.29,光合特性的表型可塑性指数均值为0.52,而酶活性和MDA含量的表型可塑性指数均值为0.57。光合生理整体表型可塑性指数最高的是464家系,最低的是490家系。【结论】福建柏各家系幼苗叶片对N、P异质环境表现出较好的适应性,K异质环境对福建柏幼苗叶片光合作用强度和酶活性有一定的抑制作用;9个家系中,464家系具有较高的光合生理可塑性。展开更多
Minimum temperatures have remarkable impacts on tree growth at high-elevation sites on the Tibetan Plateau,but the shortage of long-term and high-resolution paleoclimate records inhibits understanding of recent minimu...Minimum temperatures have remarkable impacts on tree growth at high-elevation sites on the Tibetan Plateau,but the shortage of long-term and high-resolution paleoclimate records inhibits understanding of recent minimum temperature anomalies.In this study,a warm season(April–September)reconstruction is presented for the past 467 years(1550–2016)based on Sabina tibetica ring-width chronology on the Lianbaoyeze Mountain of the central eastern Tibetan Plateau.Eight warm periods and eight cold periods were identified.Long-term minimum temperature variations revealed a high degree of coherence with nearby reconstructions.Spatial correlations between our reconstruction and global sea surface temperatures suggest that warm season minimum temperature anomalies in the central eastern Tibetan Plateau were strongly influenced by large-scale ocean atmospheric circulations,such as the El Ni?o-Southern Oscillation and the Atlantic Multidecadal Oscillation.展开更多
文摘【目的】探究在不同异质养分环境下,福建柏[Fokienia hodginsii(Dunn)Henry et Thomas]不同家系幼苗叶片光合特性和酶活性差异及变化趋势,为福建柏新品种培育提供理论依据和数据支持。【方法】以福建柏9个1年生家系(454,464,467,474,490,493,500,534,541)幼苗为研究对象,采用盆栽试验,设置氮(N)、磷(P)、钾(K)3种不同异质养分环境,以同质养分环境为对照,研究福建柏各家系幼苗叶片的光合特性、荧光参数及酶(超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT))活性和丙二醛(MDA)含量对不同养分斑块的响应差异。在此基础上,计算不同家系福建柏幼苗叶片光合特性、荧光参数及酶活性和MDA含量的表型可塑性指数。【结果】(1)N、P异质斑块下,福建柏幼苗叶片的净光合速率(P_(n))、气孔导度(G_(s))和蒸腾速率(T_(r))均高于同质养分斑块和K异质斑块,493家系在N异质斑块、541家系在P异质斑块中的P_(n)均最高。K异质斑块和同质养分斑块下,福建柏幼苗叶片胞间CO_(2)浓度(C_(i))明显高于N、P异质斑块,N异质斑块下大部分福建柏家系幼苗叶片WUE较高。(2)在N异质斑块中,不同家系福建柏幼苗叶片的初始荧光值(F_(o))和PSⅡ最大光化学量子产量(F_(v)/F_(m))均较高,PSⅡ实际光化学效率(Yield)和光合电子传递速率(ETR)呈极显著正相关,N、P异质斑块中各家系Yield和ETR均较高。各斑块下福建柏家系PSⅡ的潜在活性(F_(v)/F_(o))无明显的变化规律,同一家系的F_(v)/F_(o)峰值在各种斑块间差异较大,其中500家系的F_(v)/F_(o)在各斑块之间差异显著,534家系的F_(v)/F_(o)在各斑块间无显著差异。(3)在P异质斑块中不同家系福建柏幼苗叶片POD活性均最高,在N异质斑块中大部分家系幼苗叶片POD活性较同质养分斑块有一定提升,在K异质斑块下福建柏家系幼苗叶片POD活性较同质养分斑块无明显规律。N、P异质斑块中,各家系福建柏幼苗叶片SOD和CAT活性均高于同质养分斑块,K异质斑块中POD、SOD和CAT 3种酶活性与同质养分斑块差异不大。N、P异质斑块中,福建柏幼苗叶片MDA含量均明显低于同质养分斑块,而K异质斑块中MDA含量则明显高于N、P异质斑块。(4)福建柏家系幼苗叶片荧光参数的表型可塑性指数均值最低,为0.29,光合特性的表型可塑性指数均值为0.52,而酶活性和MDA含量的表型可塑性指数均值为0.57。光合生理整体表型可塑性指数最高的是464家系,最低的是490家系。【结论】福建柏各家系幼苗叶片对N、P异质环境表现出较好的适应性,K异质环境对福建柏幼苗叶片光合作用强度和酶活性有一定的抑制作用;9个家系中,464家系具有较高的光合生理可塑性。
基金funded by the National Key Research and Development Program of China(No.2018YFA0605601)Hong Kong Research Grants Council(No.106220169)+1 种基金the National Natural Science Foundation of China(No.42105155,41671042,and 42077417)the National Geographic Society(No.EC-95776R-22)。
文摘Minimum temperatures have remarkable impacts on tree growth at high-elevation sites on the Tibetan Plateau,but the shortage of long-term and high-resolution paleoclimate records inhibits understanding of recent minimum temperature anomalies.In this study,a warm season(April–September)reconstruction is presented for the past 467 years(1550–2016)based on Sabina tibetica ring-width chronology on the Lianbaoyeze Mountain of the central eastern Tibetan Plateau.Eight warm periods and eight cold periods were identified.Long-term minimum temperature variations revealed a high degree of coherence with nearby reconstructions.Spatial correlations between our reconstruction and global sea surface temperatures suggest that warm season minimum temperature anomalies in the central eastern Tibetan Plateau were strongly influenced by large-scale ocean atmospheric circulations,such as the El Ni?o-Southern Oscillation and the Atlantic Multidecadal Oscillation.