Human adenoviruses (HAdVs), especially HAdV-B3, -E4 and -B7, are associated with Acute Respiratory Disease in Chinese children, and occasionally in adults. In order to establish and document the profiles of the respir...Human adenoviruses (HAdVs), especially HAdV-B3, -E4 and -B7, are associated with Acute Respiratory Disease in Chinese children, and occasionally in adults. In order to establish and document the profiles of the respiratory adenovirus pathogen among children in Guangzhou, Southern China, a rapid, simple and practical method for identification and typing of respiratory adenoviruses was developed and evaluated. One pair of universal PCR primers was designed according to the conserved region of the hexon gene, which can detect not only HAdV-B3, -E4 and -B7, but also HAdV-B14, -F40 and -F41, with a specific 300bp PCR product. Three pairs of type-specific PCR primers were also designed according to the hypervariable regions of the hexon gene to type HAdV-B3, -E4 and -B7 by three independent PCR reactions, making it easy to optimize the PCR conditions. By using this method, one hundred throat swab specimens collected during Oct 2010 to Dec 2011 and suspected of being positive for adenoviral infection were identified and typed for adenoviruses. Of these samples, fifty-five were adenovirus-positive. The most common HAdV type was HAdV-B3, identified in 92.7% of samples, which is not only consistent with the data reported in 2004-2006, but also consistent with the recent report in Hangzhou, eastern China, indicating that HAdV-B3 has been circulating in Guangzhou, and maybe in eastern China, for many years. The method for the respiratory adenovirus identification and typing we developed is rapid, simple and practical, which has a potential in the real-time surveillance of circulating adenovirus strains and also to provide etiological evidence for the adenovirus-relative disease control and prevention in China.展开更多
Discrete Global Grid Systems(DGGSs) are spatial references that use a hierarchical tessellation of cells to partition and address the entire globe. They provide an organizational structure that permits fast integratio...Discrete Global Grid Systems(DGGSs) are spatial references that use a hierarchical tessellation of cells to partition and address the entire globe. They provide an organizational structure that permits fast integration between multiple sources of large and variable geospatial data sufficient for visualization and analysis. Despite a significant body of research supporting hexagonal DGGSs as the superior choice, the application thereof has been hindered owing in part to the lack of a rational hierarchy with an efficient addressing system. This paper presents an algebraic model of encoding scheme for the Aperture 3 Hexagonal(A3H) DGGS. Firstly, the definition of a grid cell, which is composed of vertices, edges, and a center, is introduced to describe fundamental elements of grids. Secondly, by identifying the grid cell with its center, this paper proves that cell centers at different levels can be represented exactly using a mixed positional number system in the complex plane through the recursive geometric relationship between two successive levels, which reveals that grid cells are essentially special complex radix numbers. Thirdly, it is shown that through the recursive geometric relationship of successive odd or even levels, the mixed positional number system can also be applied to uniquely represent cell centers at different levels under specific constraint conditions, according to which the encoding scheme is designed. Finally, it is shown that by extending the scheme to 20 triangular faces of the regular icosahedron,multi-resolution grids on closed surfaces of the icosahedron are addressed perfectly. Contrast experiments show that the proposed encoding scheme has the advantages of theoretical rigor and high programming efficiency and that the efficiency of cross-face adjacent cell searching is 242.9 times that of a similar scheme. Moreover, the proposed complex radix number representation is an ideal formalized description tool for grid systems. The research ideas introduced herein can be used to create a universal theoretical framework for DGGSs.展开更多
基金supported by National Natural Science Foundation of China (31100133)Natural Science Foundation of Guangdong Province (S2012010009261)+2 种基金Guangdong College Students' Innovative Experimental Project(1212111023)Extracurricular Research Activities of Southern Medical University (2010kw076)School of Public Health and Tropical Medicine (GWXS20110102)
文摘Human adenoviruses (HAdVs), especially HAdV-B3, -E4 and -B7, are associated with Acute Respiratory Disease in Chinese children, and occasionally in adults. In order to establish and document the profiles of the respiratory adenovirus pathogen among children in Guangzhou, Southern China, a rapid, simple and practical method for identification and typing of respiratory adenoviruses was developed and evaluated. One pair of universal PCR primers was designed according to the conserved region of the hexon gene, which can detect not only HAdV-B3, -E4 and -B7, but also HAdV-B14, -F40 and -F41, with a specific 300bp PCR product. Three pairs of type-specific PCR primers were also designed according to the hypervariable regions of the hexon gene to type HAdV-B3, -E4 and -B7 by three independent PCR reactions, making it easy to optimize the PCR conditions. By using this method, one hundred throat swab specimens collected during Oct 2010 to Dec 2011 and suspected of being positive for adenoviral infection were identified and typed for adenoviruses. Of these samples, fifty-five were adenovirus-positive. The most common HAdV type was HAdV-B3, identified in 92.7% of samples, which is not only consistent with the data reported in 2004-2006, but also consistent with the recent report in Hangzhou, eastern China, indicating that HAdV-B3 has been circulating in Guangzhou, and maybe in eastern China, for many years. The method for the respiratory adenovirus identification and typing we developed is rapid, simple and practical, which has a potential in the real-time surveillance of circulating adenovirus strains and also to provide etiological evidence for the adenovirus-relative disease control and prevention in China.
基金supported by the National Natural Science Foundation of China (Grant No. 41671410)the Postdoctoral Science Foundation of China (Grant No. 2013T60161)the Excellent Young Scholar Foundation of Information Engineering University (Grant No. 2016610802)
文摘Discrete Global Grid Systems(DGGSs) are spatial references that use a hierarchical tessellation of cells to partition and address the entire globe. They provide an organizational structure that permits fast integration between multiple sources of large and variable geospatial data sufficient for visualization and analysis. Despite a significant body of research supporting hexagonal DGGSs as the superior choice, the application thereof has been hindered owing in part to the lack of a rational hierarchy with an efficient addressing system. This paper presents an algebraic model of encoding scheme for the Aperture 3 Hexagonal(A3H) DGGS. Firstly, the definition of a grid cell, which is composed of vertices, edges, and a center, is introduced to describe fundamental elements of grids. Secondly, by identifying the grid cell with its center, this paper proves that cell centers at different levels can be represented exactly using a mixed positional number system in the complex plane through the recursive geometric relationship between two successive levels, which reveals that grid cells are essentially special complex radix numbers. Thirdly, it is shown that through the recursive geometric relationship of successive odd or even levels, the mixed positional number system can also be applied to uniquely represent cell centers at different levels under specific constraint conditions, according to which the encoding scheme is designed. Finally, it is shown that by extending the scheme to 20 triangular faces of the regular icosahedron,multi-resolution grids on closed surfaces of the icosahedron are addressed perfectly. Contrast experiments show that the proposed encoding scheme has the advantages of theoretical rigor and high programming efficiency and that the efficiency of cross-face adjacent cell searching is 242.9 times that of a similar scheme. Moreover, the proposed complex radix number representation is an ideal formalized description tool for grid systems. The research ideas introduced herein can be used to create a universal theoretical framework for DGGSs.